Application and development of fluorescence probes in MINFLUX nanoscopy

Journal of Innovative Optical Health Sciences, Ahead of Print. The MINimal emission FLUXes (MINFLUX) technique in optical microscopy, widely recognized as the next innovative fluorescence microscopy method, claims a spatial resolution of 1 –3[math]nm in both dead and living cells. To make use of the full resolution of the MINFLUX microscope, it is important to select appropriate fluorescence probes and labeling strategies, especially in living-cell imaging. This paper mainly focuses on recent applications and developments of fluores cence probes and the relevant labeling strategy for MINFLUX microscopy. Moreover, we discuss the deficiencies that need to be addressed in the future and a plan for the possible progression of MINFLUX to help investigators who have been involved in or are just starting in the field of super-resoluti on imaging microscopy with theoretical support.
Source: Journal of Innovative Optical Health Sciences - Category: Biomedical Science Authors: Source Type: research