Molecules, Vol. 27, Pages 6593: Two Ways to Achieve the Same Goal & mdash;Two Validated Quantitative NMR Strategies for a Low-Abundance Natural Product in Standardized Extracts: The Case of Hepatodamianol in Turnera diffusa

Molecules, Vol. 27, Pages 6593: Two Ways to Achieve the Same Goal—Two Validated Quantitative NMR Strategies for a Low-Abundance Natural Product in Standardized Extracts: The Case of Hepatodamianol in Turnera diffusa Molecules doi: 10.3390/molecules27196593 Authors: Aída Parra-Naranjo Cecilia Delgado-Montemayor Ricardo Salazar-Aranda Rocío Castro-Ríos Alma L. Saucedo Noemí Waksman-Minsky The quantification of low-abundance secondary metabolites in plant extracts is an analytical problem that can be addressed by different analytical platforms, the most common being those based on chromatographic methods coupled to a high-sensitivity detection system. However, in recent years nuclear magnetic resonance (NMR) has become an analytical tool of primary choice for this type of problem because of its reliability, inherent simplicity in sample preparation, reduced analysis time, and low solvent consumption. The versatility of strategies based on quantitative NMR (qNMR), such as internal and external standards and electronic references, among others, and the need to develop validated analytical methods make it essential to compare procedures that must rigorously satisfy the analytical well-established acceptance criteria for method validation. In this work, two qNMR methods were developed for the quantification of hepatodamianol, a bioactive component of T. diffusa. The first method was based on a conventional external standard calibration, and the second...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
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