Standardization of molecular analysis techniques for dna identification of bird species of the psittacidae family

Birds of the Psittacidae family belong to one of the groups with the most negative impact from wildlife trafficking, which has consequences beyond removing these species from the wild. This work aimed to standardize DNA extraction techniques from blood, feathers, and eggshells of Psittacidae to molecular identification and help track the place of origin of the seized bird. Blood and feather samples from adult of the Turquoise-fronted Parrot, Amazona aestiva, individuals (n=5) were collected, and additionally, eggshells from nests (n=3).We tested five nucleic acid extraction techniques.DNA concentrations and purity were evaluated by fluorimetry and spectrophotometry.
Source: Forensic Science International: Genetics Supplement Series - Category: Forensic Medicine Authors: Source Type: research