Molecules, Vol. 27, Pages 5041: Antibacterial Activity of Aureonuclemycin Produced by Streptomyces & nbsp;aureus Strain SPRI-371
Molecules, Vol. 27, Pages 5041: Antibacterial Activity of Aureonuclemycin Produced by Streptomyces aureus Strain SPRI-371
Molecules doi: 10.3390/molecules27155041
Authors:
Weiguo Wang
Minkang Feng
Xiaomeng Li
Feiyu Chen
Zhihao Zhang
Wenlong Yang
Chen Shao
Liming Tao
Yang Zhang
Actinomycetes play a vital role as one of the most important natural resources for both pharmaceutical and agricultural applications. The actinomycete strain SPRI-371, isolated from soil collected in Jiangsu province, China, was classified as Streptomyces aureus based on its morphological, physiological, biochemical and molecular biological characteristics. Its bacterial activity metabolites were identified as aureonuclemycin (ANM), belonging to adenosine derivatives with the molecular formula C16H19N5O9 for ANM A and C10H13N5O3 for ANM B. Simultaneously, the industrial fermentation process of a mutated S. aureus strain SPRI-371 was optimized in a 20 m3 fermentation tank, featuring a rotation speed of 170 rpm, a pressure of 0.05 MPa, an inoculum age of 36–40 h and a dissolved oxygen level maintained at 1–30% within 40–80 h and at >60% in the later period, resulting in an ANM yield of >3700 mg/L. In the industrial separation of fermentation broth, the sulfuric acid solution was selected to adjust pH and 4# resin was used for adsorption. Then, it was resolved with 20% ethanol solution and concentrated in a vac...
Source: Molecules - Category: Chemistry Authors: Weiguo Wang Minkang Feng Xiaomeng Li Feiyu Chen Zhihao Zhang Wenlong Yang Chen Shao Liming Tao Yang Zhang Tags: Article Source Type: research