Single-cell RNA and protein profiling of immune cells from the mouse brain and its border tissues

Nature Protocols, Published online: 05 August 2022; doi:10.1038/s41596-022-00716-4This protocol enables single-cell multi-omic analysis of the mouse brain immune compartment to explore immune cell heterogeneity and activation in the healthy and diseased brain, using a workflow based on HD flow cytometry, scRNA-seq and CITE-seq.

Source: Nature Protocols - August 5, 2022 Category: Biomedical Science Authors: Isabelle Scheyltjens Hannah Van Hove Karen De Vlaminck Daliya Kancheva Jonathan Bastos M ónica Vara-Pérez Ana Rita Pombo Antunes Liesbet Martens Charlotte L. Scott Jo A. Van Ginderachter Yvan Saeys Martin Guilliams Niels Vandamme Kiavash Movahedi Source Type: research