Optimization of a Quantitative PCR Methodology for Detection of Aspergillus spp. and Rhizopus arrhizus

DiscussionThis work provides evidence for the use of a qPCR multiplex method based on SYBR Green and melting curve analysis of PCR products for the detection ofA. fumigatus,A. flavus,A. niger,A. terreus, andR. arrhizus. The proposed method is simpler and less expensive than available kits based on fluorescent probes and can be used for aiding diagnosis of the most relevant invasive filamentous fungi, particularly in low-income health care institutions.
Source: Molecular Diagnosis and Therapy - Category: Molecular Biology Source Type: research