Analyses of locomotion, wing morphology, and microbiome in Drosophila nigrosparsa after recovery from antibiotics

We found no effect of tetracycline on locomotion inDrosophila nigrosparsa and differentiation of wing shape and differences in bacterial diversity between the control and gut-restoration groups three generations after tetracycline treatment. AbstractAntibiotics, such as tetracycline, have been frequently used to cure arthropods ofWolbachia endosymbionts. After the symbionts have been removed, the hosts must recover for some generations from the side effects of the antibiotics. However, most studies do not assess the direct and indirect longer-term effects of antibiotics used to removeWolbachia, which may question the exact contribution of this endosymbiont to the effects observed. Here, we used the flyDrosophila nigrosparsa treated or not with tetracycline for three generations followed by two generations of recovery to investigate the effects of this antibiotic on the fly locomotion, wing morphology, and the gut microbiome. We found that antibiotic treatment did not affect fly locomotion two generations after being treated with the antibiotic. In addition, gut-microbiome restoration was tested as a more efficient solution to reduce the potential side effects of tetracycline on the microbiome. There was no significant difference in alpha diversity between gut restoration and other treatments, but the abundance of some bacterial taxa differed significantly between the gut-restoration treatment and the control. We conclude that inD. nigrosparsa the recovery period of two genera...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research