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Portal pneumatosis in a patient with severe Salmonella colitis
Rev Esp Enferm Dig. 2021 Jul 23. doi: 10.17235/reed.2021.8090/2021. Online ahead of print.ABSTRACT65-year-old patient with a history of ischemic stroke with residual ophthalmoparesis, positive in fecal occult blood screening, pending colonoscopy, who attended the emergency room due to 3 days with pain, abdominal distension and diarrhea. In the emergency room, he presented with diffuse abdominal pain, hemodynamic instability with acute renal failure and metabolic acidosis, with severe elevation of acute phase reactants. Abdominal fluid resuscitation and CT (computed tomography) were performed, which reported dilatation of t...
Source: Revista Espanola de Enfermedades Digestivas - July 23, 2021 Category: Gastroenterology Authors: Jaume Ortega Alcaide S ílvia Pérez Farré Jose Enrique Sierra Gra ñón Jorge Juan Olsina Kissler Source Type: research

A Comparison of Three Different Bioinformatics Analyses of the 16S –23S rRNA Encoding Region for Bacterial Identification
Conclusion The higher resolution at the species level identification provided by 16S–23S rRNA encoding region NGS makes its use in routine diagnostic microbiology potentially attractive. Particularly, data analysis is one of the most important steps of a diagnostic workflow, which requires an optimal pipeline for the interpretation of the sequencing data in a short time. This study demonstrates that de novo assembly and subsequent BLASTN analysis using an in-house developed database compared to OTU clustering and mapping approaches is the most accurate and fastest approach for identification of bacterial pathogens....
Source: Frontiers in Microbiology - April 15, 2019 Category: Microbiology Source Type: research