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Source: Atherosclerosis
Condition: Thrombosis
Infectious Disease: Gastroenteritis
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Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM
In this study, we cloned the nattokinase gene from the Bacillus subtilis strain into the pET32a vector and expressed the protein in the E. coli BL21(DE3) strain. The active recombinant nattokinase was purified using Ni-NTA affinity chromatography and then evaluated for fibrinolytic and blood clot lysis activity. Physiological parameters for optimizing protein production at optimal pH, temperature, IPTG concentration, and incubation time were investigated. A statistical technique was used to optimize media components for nattokinase overproduction, and Central Composite Design-Response Surface Methodology-based optimization...
Source: Atherosclerosis - November 15, 2022 Category: Cardiology Authors: Akhilesh Modi Ishan Raval Pooja Doshi Madhvi Joshi Chaitanya Joshi Amrutlal K Patel Source Type: research
