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Slow relaxation during and after perturbation of bistable kinetics of gene expression.
Abstract After a short perturbation of a bistable genetic network, it returns to its initial steady state or transits to another steady state. The time scale characterizing such transient regimes can be appreciably longer compared to those of the degradation of the perturbed mRNAs and proteins. The author shows in detail the specifics of this slowdown of the transient kinetics using mean-field kinetic equations and Monte Carlo simulations. Attention is focused on nanocarrier-mediated delivery and release of short non-coding RNA (e.g., miRNA or siRNA) into cells with subsequent suppression of the populations of the...
Source: European Biophysics Journal : EBJ - March 21, 2019 Category: Physics Authors: Zhdanov VP Tags: Eur Biophys J Source Type: research

Inability of DNAzymes to cleave RNA in vivo is due to limited MgFormula: see text concentration in cells.
Abstract Sequence specific cleavage of RNA can be achieved by hammerhead ribozymes as well as DNAzymes. They comprise a catalytic core sequence flanked by regions that form double strands with complementary RNA. While different types of ribozymes have been discovered in natural organisms, DNAzymes derive from in vitro selection. Both have been used for therapeutic down-regulation of harmful proteins by reducing drastically the corresponding mRNA concentration. A priori DNAzymes appear advantageous because of the higher haemolytic stability and better cost effectiveness when compared to RNA. In the present work the...
Source: European Biophysics Journal : EBJ - December 16, 2017 Category: Physics Authors: Victor J, Steger G, Riesner D Tags: Eur Biophys J Source Type: research

Sigma-1 receptors modulate neonatal Nav1.5 ion channels in breast cancer cell lines.
Abstract The main aim of this study was to investigate a possible functional connection between sigma-1 receptors and voltage-gated sodium channels (VGSCs) in human breast cancer cells. The hypothesis was that sigma-1 drugs could alter the metastatic properties of breast cancer cells via the VGSC. Evidence was found for expression of sigma-1 receptor and neonatal Nav1.5 (nNav1.5) expression in both MDA-MB-231 and MDA-MB-468 cells. Sigma-1 drugs (SKF10047 and dimethyltryptamine) did not affect cell proliferation or migration but significantly reduced adhesion to the substrate. Silencing sigma-1 receptor expression ...
Source: European Biophysics Journal : EBJ - May 8, 2016 Category: Physics Authors: Aydar E, Stratton D, Fraser SP, Djamgoz MB, Palmer C Tags: Eur Biophys J Source Type: research