• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Dexamethasone may inhibit placental growth by blocking glucocorticoid receptors via phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin and reactive oxygen species/AMP-activated protein kinase signalling pathways in human placental JEG-3 cells
This study explored the molecular mechanism underlying the effects of dexamethasone (DEX, 1µM) on glucose transporters (GLUT) in JEG-3 human placental choriocarcinoma cells. JEG-3 cells were treated with DEX, an expression plasmid encoding human glucocorticoid receptor α (GRα), pcDNA3.1-GRα, GRα short interference (si) RNA, LY294002, xanthine oxidase (XO)/hypoxanthine (HX), rapamycin, insulin-like growth factor (IGF) 1, N-acetylcysteine (NAC) or phosphatidic acid (PA), and cell proliferation, apoptosis, mitochondrial membrane potential (MMP), human chorionic gonadotrophin (hCG) content, human placental lactogen (hPL) ...
Source: Reproduction, Fertility, and Development - August 16, 2021 Category: Reproduction Medicine Authors: Xin Zhan Yiran Xie Liping Sun Qi Si Hongkai Shang Source Type: research

The distribution and expression of vascular endothelial growth factor A (VEGFA) during follicular development and atresia in the pig.
This study investigated the distribution and expression of VEGFA during porcine follicular development and atresia. Pig ovaries were obtained, individual medium-sized (3-5mm in diameter) antral follicles were separated and classified into healthy, early atretic or progressively atretic groups. Immunobiology and quantitative techniques were used to investigate the varied follicular distribution of VEGFA at both the morphological and molecular level. The results indicated that VEGFA protein expression peaked in tertiary follicles, mostly distributed in the thecal and inner granulosa layers, during follicular development whil...
Source: Reproduction, Fertility, and Development - September 23, 2019 Category: Reproduction Medicine Authors: Gao X, Zhang J, Pan Z, Li Q, Liu H Tags: Reprod Fertil Dev Source Type: research

Role of epithelial-mesenchymal transition regulated by twist basic helix-loop-helix transcription factor 2 (Twist2) in embryo implantation in mice.
Abstract In a previous study we found the expression of epithelial-mesenchymal transition (EMT) biomarkers, including E-cadherin and N-cadherin, was significantly altered in uterine endometrium during embryo implantation via regulation by microRNA (miRNA)-429 and protocadherin-8 (Pcdh8). As a natural continuation of the previous study, the aim of the present study was to explore the role of EMT during embryo implantation and the potential activity of twist basic helix-loop-helix transcription factor 2 (Twist2) in regulating embryo implantation. A pregnancy model was established by naturally mating adult female ICR...
Source: Reproduction, Fertility, and Development - February 20, 2019 Category: Reproduction Medicine Authors: Gou J, Hu T, Li L, Xue L, Zhao X, Yi T, Li Z Tags: Reprod Fertil Dev Source Type: research

De novo transcription of thyroid hormone receptors is essential for early bovine embryo development in vitro.
In conclusion, THRs are transcribed soon after fertilisation, before major activation of the embryonic genome, and they are essential for bovine embryo development in vitro. PMID: 29179810 [PubMed - as supplied by publisher]
Source: Reproduction, Fertility, and Development - November 28, 2017 Category: Reproduction Medicine Authors: Rho NY, Ashkar FA, Revay T, Madan P, Rho GJ, King WA, Favetta LA Tags: Reprod Fertil Dev Source Type: research

Mouse double minute homologue 2 (MDM2) downregulation by miR-661 impairs human endometrial epithelial cell adhesive capacity.
This study highlights a potential new mechanism by which human blastocyst-secreted miR-661 reduces endometrial epithelial cell adhesion; via downregulation of MDM2. These findings suggest that MDM2 contributes to endometrial-blastocyst adhesion, implantation and infertility in women. PMID: 28847363 [PubMed - as supplied by publisher]
Source: Reproduction, Fertility, and Development - August 29, 2017 Category: Reproduction Medicine Authors: Winship A, Ton A, Van Sinderen M, Menkhorst E, Rainczuk K, Griffiths M, Cuman C, Dimitriadis E Tags: Reprod Fertil Dev Source Type: research

Endo-siRNA deficiency results in oocyte maturation failure and apoptosis in porcine oocytes.
In this study, by knockdown of DICER1, DROSHA and its cofactor DiGeorge syndrome critical region 8 (DGCR8) in porcine oocytes, we found that the proportion of oocytes with DICER1 deficiency that developed to meiosis II (MII) stage was significantly lower than oocytes with DROSHA and DGCR8 deficiency (39.23 versus 68.71 and 71.25% respectively; P<0.05). Oocytes lacking DROSHA and DGCR8 formed a barrel-shaped metaphase I spindle, with chromosomes tightly aligned at the metaphase plate whereas most oocytes (87%) lacking DICER1 showed spindle abnormalities during oocyte in vitro maturation. Furthermore, DICER1 deficiency al...
Source: Reproduction, Fertility, and Development - April 12, 2017 Category: Reproduction Medicine Authors: Liu W, Zhao Q, Piao S, Wang C, Kong Q, An T Tags: Reprod Fertil Dev Source Type: research

Stathmin 1 plays a role in endometrial decidualisation by regulating hypoxia inducible factor-1? and vascular endothelial growth factor during embryo implantation.
Abstract The aim of the present study was to explore the potential mechanism underlying stathmin 1 (Stmn1) regulation of embryo implantation, as a continuation of previous proteomic research. Adult healthy female mice were mated naturally with fertile males. Murine uterine tissue was collected during the peri-implantation period. Local expression of Stmn1 during embryo implantation was detected by immunohistochemistry (IHC), which showed that Stmn1 was extensively expressed in endometrial glandular epithelium, vascular endothelium, luminal epithelium and the underlying stromal cells at the implantation site on Day...
Source: Reproduction, Fertility, and Development - July 25, 2016 Category: Reproduction Medicine Authors: Gou J, Jia J, Feng J, Zhao X, Yi T, Cui T, Li Z Tags: Reprod Fertil Dev Source Type: research

Optineurin suppression activates the mediators involved in the terminal effector pathways of human labour and delivery.
Abstract Spontaneous preterm birth remains the major cause of neonatal death and morbidity. Studies in non-gestational tissues report that optineurin (OPTN) is critical in the termination of NFKB1 activity and control of inflammation, central features of spontaneous preterm birth. The aims of the present study were to determine: (1) OPTN expression in fetal membranes and the myometrium during labour; (2) the effects of IL1B on OPTN expression in primary myometrial cells; and (3) the effects of OPTN short interference (si) RNA on IL1B-stimulated proinflammatory and prolabour mediators. OPTN mRNA and protein express...
Source: Reproduction, Fertility, and Development - May 1, 2016 Category: Reproduction Medicine Authors: Lim R, Barker G, Lappas M Tags: Reprod Fertil Dev Source Type: research

Potential effects of interferon regulatory factor 4 in a murine model of polyinosinic-polycytidylic acid-induced embryo resorption.
Abstract Interferon regulatory factor (IRF) 4 has been reported to modulate Toll-like receptor (TLR) signalling. Polyinosinic-polycytidylic acid (poly(I:C)) can be specifically recognised by TLR3, triggering the innate immune response and subsequently resulting in pregnancy loss. In the present study, poly(I:C) was administered to mice with or without TLR3 blockade. Chemokine (C-X-C motif) receptor 4 (CXCR4) expression was measured with or without chemokine (C-X-C motif) ligand 12 (CXCL12) inhibition. In cultured murine splenic mononuclear cells, IRF4 was knocked down by a specific short interference (si) RNA. IRF...
Source: Reproduction, Fertility, and Development - April 15, 2015 Category: Reproduction Medicine Authors: Wang J, Yin T, Wen Y, Tian F, He X, Zhou D, Lin Y, Yang J Tags: Reprod Fertil Dev Source Type: research