• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Interaction of galectin-7 with HMGCS1 in vitro may facilitate cholesterol deposition in cultured keratinocytes
Three-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase 1 (HMGCS1) was identified to interact with Gal-7, a pro-apoptotic β-galactoside binding protein, by yeast two-hybrid system. Their interaction was confirmed by in vitro β-galactosidase, BIA core and immunoprecipitation assays. Distinct interactive site of HMGCS1was found to reside at Phe-26. The expression of HMGCS1 in cultured keratinocytes was up-regulated by exogenous Gal-7 and down-regulated in Gal-7 siRNA transfected cells. HMGCS1-overexpressing cells were found to induce Gal-7 expression, which suggests that Gal-7 and HMGCS1 expressions are both stimulate...
Source: Journal of Investigative Dermatology - August 26, 2021 Category: Dermatology Authors: Norihiro Fujimoto, Minoru Akiyama, Yasushi Satoh, Shingo Tajima Tags: Original Article Source Type: research

132 Identification of novel pathways linked to the pathogenesis of Recessive X-Linked Ichthyosis
Recessive X-Linked Ichthyosis (RXLI) results from a deficiency of steroid sulfatase (STS) and is characterized by an accumulation of cholesterol sulfate in the upper layers of the epidermis, resulting in a scaling phenotype and barrier dysfunction. To further understand the pathomechanisms of RXLI, we have generated an RNA-Seq data set of the transcriptome of keratinocytes with siRNA-induced loss of STS. To validate these data, we generated a 3D XLRI model using 2 telomerase-immortalized keratinocyte cell lines with stable knockdown of STS using different lentiviral shRNA clones.
Source: Journal of Investigative Dermatology - August 16, 2016 Category: Dermatology Authors: F. McGeoghan, M. Menon, P. Dewan, M. Caley, M. Donaldson, E.A. O ’Toole Tags: Epidermal Structure and Function Source Type: research

375 Modelling and understanding pathomechanisms of X-linked recessive ichthyosis in vitro
X-Linked Recessive Ichthyosis (XLRI) results from a deficiency of steroid sulfatase (STS) and is characterized by an accumulation of cholesterol sulfate in the upper layers of the epidermis, resulting in a scaling phenotype and barrier dysfunction. We have generated an RNA-Seq data set of the transcriptome of keratinocytes with siRNA-induced loss of the enzyme steroid sulfatase (STS) to further understand the pathomechanisms of XLRI. To validate these data, we generated a 3D XLRI model using 2 telomerase-immortalized keratinocyte cell lines with stable knockdown of STS using different lentiviral shRNA clones.
Source: Journal of Investigative Dermatology - April 20, 2016 Category: Dermatology Authors: F.T. McGeoghan, E. O’Toole, M. Caley, M. Menon, P. Dewan, M. Donaldson Tags: Genetic Disease, Gene Regulation & Gene Therapy Source Type: research

405 Hydrophobically modified siRNAs (hsiRNAs) provide a platform to silence gene expression in inflammatory skin diseases
RNA interference (RNAi) is a highly conserved biological process in which small RNA molecules inhibit gene expression. It has great potential to treat different human diseases, including inflammatory skin disorders. Despite promising results in vitro, therapeutic application of RNAi has been limited mainly due to difficulties in efficient delivery of the therapeutic oligonucleotides to target cells. Here we describe that direct conjugation of fully stabilized siRNA (hsiRNAs) to cholesterol (Chol) or biologically occurring lipids results in widespread distribution in the skin following local administration.
Source: Journal of Investigative Dermatology - April 20, 2016 Category: Dermatology Authors: M. Rashighi, M. Nikan, A.H. Coles, A. Khvorova, J.E. Harris Tags: Genetic Disease, Gene Regulation & Gene Therapy Source Type: research