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Inhibition of γ/β Globin Gene Switching in CD 34 +  Derived Erythroid Cells by BCL11A RNA Silencing
In this study, the possibility of increasing HbF in the CD34+ derived erythroid cells was investigated by BCL11A inhibition using specific small-interfering RNAs (siRNAs). Human peripheral blood-derived hematopoietic stem cells were isolated and differentiated to erythroid cells. Erythroid maturation was investigated using cell morphology parameters and flow cytometry analysis of CD235a expression On day 20, siRNA complementary to BCL11A was transfected to differentiating cells via electroporation. BCL11A expression was evaluated through real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and e...
Source: Indian Journal of Hematology and Blood Transfusion - May 12, 2019 Category: Hematology Source Type: research