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Sonoporation as an Approach for siRNA delivery into T cells
Delivery of small interfering RNAs (siRNAs) into primary T cells is quite challenging because they are non-proliferating cells and are difficult to transfect with non-viral approaches. Because sonoporation is independent of the proliferation status of cells and siRNA acts in the cell cytoplasm, we investigated whether sonoporation could be used to deliver siRNA into mouse and human T cells. Cells mixed with Definity microbubbles and siRNA were sonicated with a non-focused transducer of center frequency 2.20 MHz producing ultrasound at a 10% duty cycle, pulse repetition frequency of 2.20 kHz and spatial average temporal ave...
Source: Ultrasound in Medicine and Biology - September 16, 2019 Category: Radiology Authors: Alina Karki, Emily Giddings, Ana Carreras, Devin Champagne, Karen Fortner, Mercedes Rincon, Junru Wu Tags: Original Contribution Source Type: research

Efficient siRNA Delivery Using Novel Cell-Penetrating Peptide-siRNA Conjugate-Loaded Nanobubbles and Ultrasound
In this study, development of a novel siRNA delivery system based on ultrasound-sensitive nanobubbles (NBs, nano-sized echogenic liposomes) and cell-permeable peptides (CPPs) is described. A CPP-siRNA conjugate was entrapped in an NB, (CPP-siRNA)-NB, and the penetration of CPP-siRNA was temporally masked; local ultrasound stimulation triggered the release of CPP-siRNA from the NBs and activated its penetration.
Source: Ultrasound in Medicine and Biology - March 20, 2016 Category: Radiology Authors: Xiangyang Xie, Wen Lin, Mingyuan Li, Yang Yang, Jianping Deng, Hui Liu, Ying Chen, Xudong Fu, Hong Liu, Yanfang Yang Tags: Original Contribution Source Type: research

Acoustic Cavitation-Mediated Delivery of Small Interfering Ribonucleic Acids with Phase-Shift Nano-emulsions
Localized, targeted delivery of small interfering ribonucleic acid (siRNA) has been the foremost hurdle in the use of siRNA for the treatment of various diseases. Major advances have been achieved in the synthesis of siRNA, which have led to greater target messenger RNA (mRNA) silencing and stability under physiologic conditions. Although numerous delivery strategies have shown promise, there are still limited options for targeted delivery and release of siRNA administered systemically. In this in vitro study, phase-shift nano-emulsions (PSNE) were explored as cavitation nuclei to facilitate free siRNA delivery to cancer ...
Source: Ultrasound in Medicine and Biology - May 13, 2015 Category: Radiology Authors: Mark T. Burgess, Tyrone M. Porter Tags: Original Contribution Source Type: research

Ultrasound and Microbubble-Targeted Delivery of Small Interfering RNA Into Primary Endothelial Cells Is More Effective Than Delivery of Plasmid DNA
Abstract: Ultrasound and microbubble-targeted delivery (UMTD) is a promising non-viral technique for genetic-based therapy. We found that UMTD of small interfering RNA (siRNA) is more effective than delivery of plasmid DNA (pDNA). UMTD (1 MHz, 0.22 MPa) of fluorescently labeled siRNA resulted in 97.9 ± 1.5% transfected cells, with siRNA localized homogenously in the cytoplasm directly after ultrasound exposure. UMTD of fluorescently labeled pDNA resulted in only 43.0 ± 4.2% transfected cells, with localization mainly in vesicular structures, co-localizing with endocytosis markers clathrin and caveolin. Delivery of siRNA ...
Source: Ultrasound in Medicine and Biology - December 23, 2013 Category: Radiology Authors: Lynda J.M. Juffermans, Bernadet D.M. Meijering, Robert H. Henning, Leo E. Deelman Tags: Original Contributions Source Type: research

Preparation of Protamine Cationic Nanobubbles and Experimental Study of Their Physical Properties and In Vivo Contrast Enhancement
In this study, we aimed to prepare a novel type of microbubble (MB), protamine cationic nanobubble (NB), to provide a new vector for tumor gene therapy. We prepared cationic NBs with protamine and other lipid components using mechanical oscillation. The protamine cationic NBs had a mean diameter of 521.2 ± 37.57 nm, a zeta potential of +18.5 mV, and a gene-carrying capacity of 15.69 μg androgen receptor (AR) siRNA per 108 NBs. The cationic NBs exhibited superior contrast enhancement for in vivo imaging compared with SonoVue (Bracco, Geneva, Switzerland), and their physical properties did not change significantly a...
Source: Ultrasound in Medicine and Biology - August 12, 2013 Category: Radiology Authors: Hai-Peng Tong, Luo-Fu Wang, Yan-Li Guo, Lang Li, Xiao-Zhou Fan, Jun Ding, Hai-Yun Huang Tags: Original Contributions Source Type: research