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Large-conductance calcium-activated potassium channels mediate lipopolysaccharide-induced activation of murine microglia Immunology
Large-conductance calcium-activated potassium (BK) channels are ubiquitously expressed in most cell types where they regulate many cellular, organ, and organismal functions. Although BK currents have been recorded specifically in activated murine and human microglia, it is not yet clear whether and how the function of this channel is related to microglia activation. Here, using patch-clamping, Griess reaction, ELISA, immunocytochemistry, and immunoblotting approaches, we show that specific inhibition of the BK channel with paxilline (10 μm) or siRNA-mediated knockdown of its expression significantly suppresses lipopolysac...
Source: Journal of Biological Chemistry - August 29, 2019 Category: Chemistry Authors: Xiaoying Yang, Guiqin Wang, Ting Cao, Li Zhang, Yunzhi Ma, Shuhui Jiang, Xinchen Teng, Xiaohui Sun Tags: Neurobiology Source Type: research

F-box protein-32 down-regulates small-conductance calcium-activated potassium channel 2 in diabetic mouse atria Molecular Bases of Disease
In conclusion, DM-induced SK2 channel down-regulation appears to be due to an FBXO-32-dependent increase in UPS-mediated SK2 protein degradation.
Source: Journal of Biological Chemistry - March 14, 2019 Category: Chemistry Authors: Tian-You Ling, Fu Yi, Tong Lu, Xiao-Li Wang, Xiaojing Sun, Monte S. Willis, Li-Qun Wu, Win-Kuang Shen, John P. Adelman, Hon-Chi Lee Tags: Molecular Bases of Disease Source Type: research

Regulation of Kv11.1 potassium channel C-terminal isoform expression by the RNA-binding proteins HuR and HuD RNA
In this study, using HEK293 cells and reporter gene expression, pulldown assays, and RNase protection assays, we identified the RNA-binding proteins Hu antigen R (HuR) and Hu antigen D (HuD) as regulators of Kv11.1 isoform expression. We show that HuR and HuD inhibit activity at the intron 9 polyadenylation site. When co-expressed with the KCNH2 gene, HuR and HuD increased levels of the Kv11.1a isoform and decreased the Kv11.1a-USO isoform in the RNase protection assays and immunoblot analyses. In patch clamp experiments, HuR and HuD significantly increased the Kv11.1 current. siRNA-mediated knockdown of HuR protein decrea...
Source: Journal of Biological Chemistry - December 21, 2018 Category: Chemistry Authors: Qiuming Gong, Matthew R. Stump, Zhengfeng Zhou Tags: Membrane Biology Source Type: research

Complement 1q-like-3 protein inhibits insulin secretion from pancreatic {beta}-cells via the cell adhesion G protein-coupled receptor BAI3 Cell Biology
Secreted proteins are important metabolic regulators in both healthy and disease states. Here, we sought to investigate the mechanism by which the secreted protein complement 1q-like-3 (C1ql3) regulates insulin secretion from pancreatic β-cells, a key process affecting whole-body glucose metabolism. We found that C1ql3 predominantly inhibits exendin-4– and cAMP-stimulated insulin secretion from mouse and human islets. However, to a lesser extent, C1ql3 also reduced insulin secretion in response to KCl, the potassium channel blocker tolbutamide, and high glucose. Strikingly, C1ql3 did not affect insulin secretion stimula...
Source: Journal of Biological Chemistry - November 23, 2018 Category: Chemistry Authors: Rajesh Gupta, Dan C. Nguyen, Michael D. Schaid, Xia Lei, Appakalai N. Balamurugan, G. William Wong, Jeong-a Kim, James E. Koltes, Michelle E. Kimple, Sushant Bhatnagar Tags: Metabolism Source Type: research