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Total 1 results found since Jan 2013.
The knockdown efficiency of telomere associated genes with specific methodology in a zebrafish cell line
Biochimie. 2021 Jun 29:S0300-9084(21)00158-9. doi: 10.1016/j.biochi.2021.06.013. Online ahead of print.ABSTRACTZebrafish is broadly used as a model organism in gene loss-of-function studies in vivo, but its employment in vitro is greatly limited by the lack of efficient gene knockdown approaches in zebrafish cell lines such as ZF4. In this article, we attempt to induce silencing of telomere associated genes in ZF4 by applying the frequently-used siRNA transfection technology and a novel moiety-linked morpholino(vivo-MO). By proceeding with integrated optimization of siRNAs transfection and vivo-MOs treatment, we compared f...
Source: Biochimie - July 2, 2021 Category: Biochemistry Authors: Xuefei Hu Shuaiyun Gao Peng Wang Yulin Zhou Kehua Chen Qiaowen Chen Bo Wang Weiguo Hu Peng Cheng Rita Eid Marie-Jos èph Giraud-Panis Lei Wang Eric Gilson Jing Ye Yiming Lu Source Type: research
