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Mutation Frequencies in HIV-1 Genome in Regions Containing Efficient RNAi Targets As Calculated from Ultra-Deep Sequencing Data.
Abstract HIV-1 is one of the most variable viruses. The development of gene therapy technology using RNAi for AIDS/HIV-1 treatment is a potential alternative for traditional anti-retroviral therapy. Anti-HIV-1 siRNA should aim to exploit the most conserved viral targets. Using the deep sequencing of potential RNAi targets in 100-nt HIV-1 genome fragments from the clinical HIV-1 subtype A isolates in Russia, we found that the frequencies of all possible transversions and transitions in certain RNAi targets are 3-38 times lower than in adjacent sequences. Therefore, these targets are conserved. We propose the develo...
Source: Molekuliarnaia Biologiia - May 1, 2018 Category: Molecular Biology Authors: Kretova OV, Gorbacheva MA, Fedoseeva DM, Kravatsky YV, Chechetkin VR, Tchurikov NA Tags: Mol Biol (Mosk) Source Type: research