• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii.
In this study, the full-length 1,059 bp cDNA sequence of Acanthamoeba castellanii PRMT1 (AcPRMT1) was cloned for the first time. The AcPRMT1 protein comprised of 352 amino acids with a SAM-dependent methyltransferase PRMT-type domain. The expression level of AcPRMT1 was highly increased during encystation of A. castellanii. The EGFP-AcPRMT1 fusion protein was distributed over the cytoplasm, but it was mainly localized in the nucleus of Acanthamoeba. Knock down of AcPRMT1 by synthetic siRNA with a complementary sequence failed to form mature cysts. These findings suggested that AcPRMT1 plays a critical role in the regulatio...
Source: Korean Journal of Parasitology - May 17, 2017 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Identification of Protein Arginine Methyltransferase 5 as a Regulator for Encystation of Acanthamoeba.
Authors: Moon EK, Hong Y, Chung DI, Goo YK, Kong HH Abstract Encystation is an essential process for Acanthamoeba survival under nutrient-limiting conditions and exposure to drugs. The expression of several genes has been observed to increase or decrease during encystation. Epigenetic processes involved in regulation of gene expression have been shown to play a role in several pathogenic parasites. In the present study, we identified the protein arginine methyltransferase 5 (PRMT5), a known epigenetic regulator, in Acanthamoeba castellanii. PRMT5 of A. castellanii (AcPRMT5) contained domains found in S-adenosylmeth...
Source: Korean Journal of Parasitology - May 20, 2016 Category: Parasitology Tags: Korean J Parasitol Source Type: research