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Viruses, Vol. 14, Pages 339: In Vitro Inhibition of Replication of Dengue Virus Serotypes 1 & ndash;4 by siRNAs Bound to Non-Toxic Liposomes
The objective herein was to design and evaluate in vitro siRNAs bound to liposomes that inhibit DENV replication. siRNAs were designed against DENV1–4 from conserved regions using siDirect2.0 and Web-BLOCK-iT™ RNAiDesigner; the initial in vitro evaluation was carried out through transfection into HepG2 cells. siRNA with silencing capacity was encapsulated in liposomes composed of D-Lin-MC3-DMA, DSPC, Chol. Cytotoxicity, hemolysis, pro-inflammatory cytokine release and antiviral activity were evaluated using plaque assay and RT-qPCR. A working concentration of siRNA was established at 40 nM. siRN...
Source: Viruses - February 7, 2022 Category: Virology Authors: Rodriguez-Salazar Recalde-Reyes Bedoya Padilla-Sanabria Casta ño-Osorio Giraldo Tags: Article Source Type: research

Viruses, Vol. 13, Pages 1066: An Aedes Aegypti-Derived Ago2 Knockout Cell Line to Investigate Arbovirus Infections
l Esther Schnettler Mosquitoes are known as important vectors of many arthropod-borne (arbo)viruses causing disease in humans. These include dengue (DENV) and Zika (ZIKV) viruses. The exogenous small interfering (si)RNA (exo-siRNA) pathway is believed to be the main antiviral defense in arthropods, including mosquitoes. During infection, double-stranded RNAs that form during viral replication and infection are cleaved by the enzyme Dicer 2 (Dcr2) into virus-specific 21 nt vsiRNAs, which are subsequently loaded into Argonaute 2 (Ago2). Ago2 then targets and subsequently cleaves complementary RNA sequences, resulting i...
Source: Viruses - June 3, 2021 Category: Virology Authors: Christina Scherer Jack Knowles Vattipally B. Sreenu Anthony C. Fredericks Janina Fuss Kevin Maringer Ana Fernandez-Sesma Andres Merits Margus Varjak Alain Kohl Esther Schnettler Tags: Article Source Type: research

Viruses, Vol. 11, Pages 540: Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, Aedes aegypti
Failloux Although the role of exogenous small interfering RNA (siRNA) and P-element induced wimpy testis (PIWI)-interacting RNA (piRNA) pathways in mosquito antiviral immunity is increasingly better understood, there is still little knowledge regarding the role of mosquito cellular microRNA (miRNA). Identifying direct interactions between the mosquito miRNAs and the RNA genome of arboviruses and choosing the relevant miRNA candidates to explore resulting antiviral mechanisms are critical. Here, we carried out genomic analyses to identify Aedes aegypti miRNAs that potentially interact with various lineages and genotyp...
Source: Viruses - June 9, 2019 Category: Virology Authors: Yen Chen Sreenu Kohl Failloux Tags: Article Source Type: research

Viruses, Vol. 11, Pages 335: Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells
-Yee Chee Recent evidence has demonstrated that dengue virus requires active filopodia formation for a successful infection. However, the cellular factor involved in the interaction has not been fully elucidated. We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin II as a dengue virus serotype 2 (DENV2) interacting protein on Vero cells, upon filopodia induction. Flow cytometry analysis showed annexin II on the Vero cells surface increased when DENV2 was added. The amount of annexin II in the plasma membrane fraction was reduced as the infection progressed. Antibody-mediate...
Source: Viruses - April 8, 2019 Category: Virology Authors: Isah Abubakar Aliyu King-Hwa Ling Nur Fariesha Md Hashim Jia-Yong Lam Hui-Yee Chee Tags: Article Source Type: research