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cyp51A gene silencing using RNA interference in azole‐resistant Aspergillus fumigatus
Summary An increasing number of reports have described the emergence of acquired resistance of Aspergillus fumigatus to azole compounds. The primary mechanism of resistance in clinical isolates is the mutation of the azole drug target enzyme, which is encoded by the cyp51A gene. The aim of this study was to evaluate the impact of silencing the cyp51A gene in azole‐resistant A. fumigatus isolates. A 21‐nucleotide small‐interfering RNA (siRNA) was designed based on the cDNA sequence of the A. fumigatus cyp51A gene. After silencing the cyp51A gene in germinated conidia (15, 20, 25 and 50 nM), azole‐resistant A. fumiga...
Source: Mycoses - October 8, 2015 Category: Research Authors: Bita Mousavi, Mohammad T. Hedayati, Ladan Teimoori‐Toolabi, Jacques Guillot, Ahad Alizadeh, Hamid Badali Tags: Original Article Source Type: research