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Source: American Journal of Translational Research
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Total 412 results found since Jan 2013.
Neuroprotective effect of miR-665 against sevoflurane anesthesia-induced cognitive dysfunction in rats through PI3K/Akt signaling pathway by targeting insulin-like growth factor 2.
This study suggests that miR-665 might be explored as a potential target of therapy for sevoflurane-induced cognitive impairment.
PMID: 28386360 [PubMed]
Source: American Journal of Translational Research - April 9, 2017 Category: Research Tags: Am J Transl Res Source Type: research
Targeting the eIF4E/ β-catenin axis sensitizes cervical carcinoma squamous cells to chemotherapy.
In this study, we investigated the phosphorylation levels of eukaryotic translation initiation factor 4E (eIF4E) in cervical cancer cells subjected to chemotherapy. Results showed that chemotherapeutic drugs significantly increased eIF4E phosphorylation at S209 in HeLa and SiHa cells. Upregulation of phosphorylated eIF4E (p-eIF4E) levels has also been shown in cisplatin-resistant HeLa cells and has been observed to be a common response of cervical cancer patients undergoing chemotherapy. We further showed that chemotherapeutic drugs increase β-catenin activity and mRNA levels of Wnt/β-catenin target genes in cervical can...
Source: American Journal of Translational Research - April 9, 2017 Category: Research Tags: Am J Transl Res Source Type: research
Inhibition of autophagy results in a reversal of taxol resistance in nasopharyngeal carcinoma by enhancing taxol-induced caspase-dependent apoptosis.
Authors: Song Y, Li W, Peng X, Xie J, Li H, Tan G
Abstract
Drug resistance in nasopharyngeal carcinoma remains a major obstacle of clinical therapy. We found that the taxol-resistant cells demonstrated higher basal levels of autophagy than parental cells, which could be inhibited by 3-MA and Beclin-1-siRNA. We further revealed that inhibition of autophagy enhanced taxol-induced caspase-dependent apoptosis, resulted in partial reversal of the acquired taxol resistance in taxol-resistant cells. Our results suggest that the combination of an autophagy inhibitor with taxol may be a promising approach to promote therape...
Source: American Journal of Translational Research - May 6, 2017 Category: Research Tags: Am J Transl Res Source Type: research
Inhibition of AMPK-related kinase 5 (ARK5) enhances cisplatin cytotoxicity in non-small cell lung cancer cells through regulation of epithelial-mesenchymal transition.
Authors: Li M, Zheng C, Xu H, He W, Ruan Y, Ma J, Zheng J, Ye C, Li W
Abstract
Lung cancer incidence and mortality rates are amongst the highest of all malignant tumors worldwide. ARK5 is a member of the human AMP-activated protein kinase (AMPK) family which is implicated in tumor survival and progression. The current study was designed to explore the role of ARK5 in resistance of non-small cell lung cancer (NSCLC) to cisplatin. We studied the sensitivity of two NSCLC cell lines, NCI-H1229 and A549, to cisplatin by using proliferation and cell viability assays. We then examined expression of ARK5, Twist, and the ep...
Source: American Journal of Translational Research - May 6, 2017 Category: Research Tags: Am J Transl Res Source Type: research
GC7 blocks epithelial-mesenchymal transition and reverses hypoxia-induced chemotherapy resistance in hepatocellular carcinoma cells.
In this study, we investigated the role of GC7 in the therapeutic effect of doxorubicin in hypoxia in HCC. We utilized four types of HCC cell line (Huh7, Hep3B, SNU387 and SNU449) in this study. Western blot and immunofluorescence were used to detect expression of epithelial/mesenchymal markers for EMT evaluation and HIF-1α was knocked down using HIF-1α-siRNA. Hypoxia-induced EMT contributed to doxorubicin chemoresistance in HCC cells. Low concentrations of GC7 sensitized Huh7 and Hep3B to doxorubicin by reversing EMT. Knockdown of HIF-1α attenuated hypoxia-induced EMT and abolished the unique feature of GC7. GC7 enhanc...
Source: American Journal of Translational Research - June 2, 2017 Category: Research Tags: Am J Transl Res Source Type: research
Hypoxia-inducible factor 1 α protects mesenchymal stem cells against oxygen-glucose deprivation-induced injury via autophagy induction and PI3K/AKT/mTOR signaling pathway.
In conclusion, these data suggest that Hif-1α overexpression protects MSCs from OGD-induced injury via a mechanism in which autophagy and PI3K/AKT/mTOR pathway are implicated.
PMID: 28559999 [PubMed]
Source: American Journal of Translational Research - June 2, 2017 Category: Research Tags: Am J Transl Res Source Type: research
MicroRNA-199a-3p inhibits tumorigenesis of hepatocellular carcinoma cells by targeting ZHX1/PUMA signal.
CONCLUSIONS: miRNA-199a-3p could effectively prevent primary tumor formation. The ability of this therapy to decrease tumorigenesis may be related toZHX1-dependent PUMA signals.
PMID: 28559996 [PubMed]
Source: American Journal of Translational Research - June 2, 2017 Category: Research Tags: Am J Transl Res Source Type: research
PEA3 protects against gentamicin nephrotoxicity: role of mitochondrial dysfunction.
Authors: Chen Q, Cui Y, Ding G, Jia Z, Zhang Y, Zhang A, Huang S
Abstract
Toxin-induced nephrotoxicity is one of the major causes leading to the acute kidney injury (AKI). Among these nephrotoxic toxins, gentamicin can induce AKI with elusive mechanisms. Emerging evidence demonstrated that PEA3 (polyomavirus enhancer activator 3) contributed to the nephrogenesis, while its role in AKI remains unknown. Thus, this study was to investigate the role of PEA3 in gentamicin nephrotoxicity, as well as the underlying mechanisms. In rats, gentamicin treatment (200 mg/kg twice per day) for two days induced remarkable kidney i...
Source: American Journal of Translational Research - June 2, 2017 Category: Research Tags: Am J Transl Res Source Type: research
MicroRNA-1825 induces proliferation of adult cardiomyocytes and promotes cardiac regeneration post ischemic injury.
Authors: Pandey R, Velasquez S, Durrani S, Jiang M, Neiman M, Crocker JS, Benoit JB, Rubinstein J, Paul A, Ahmed RP
Abstract
In mammals, proliferative capacity of cardiomyocytes is lost soon after birth, while zebrafish and other lower organisms like newts are known to regenerate injured hearts even at an adult age. Here, we show that miR-1825 can induce robust proliferation of adult rat cardiomyocytes and can improve cardiac function in-vivo post myocardial infarction. Rat adult cardiomyocytes transfected with miR-1825 showed a significant increase in DNA synthesis, mitosis, cytokinesis, and an increase in cell nu...
Source: American Journal of Translational Research - July 5, 2017 Category: Research Tags: Am J Transl Res Source Type: research
Down-regulation of long non-coding RNA AFAP1-AS1 inhibits tumor cell growth and invasion in lung adenocarcinoma.
Authors: Zhuang Y, Jiang H, Li H, Dai J, Liu Y, Li Y, Miao L, Cai H, Xiao Y, Xia H, Wang Y, Shi M
Abstract
An increasing number of deregulated long non-coding RNAs (lncRNA) have been implicated in cancer in humans, suggesting that lncRNAs may be involved in tumorigenesis or tumor progression. In previous investigations, lncRNA, AFAP1-AS1 has been found to be associated with several cancers, including nasopharyngeal carcinoma, lung cancer and esophageal adenocarcinoma. However, the function of AFAP1-AS1 in lung cancer has not been reported. In our present study, we found that AFAP1-AS1 was overexpressed in lung aden...
Source: American Journal of Translational Research - July 5, 2017 Category: Research Tags: Am J Transl Res Source Type: research
Vasohibin 2 as a potential predictor of aggressive behavior of triple-negative breast cancer.
Authors: Wang B, Yang L, Zhao Q, Zhu L
Abstract
Triple-negative breast cancer (TNBC) is a subtype breast cancer with aggressive behavior, advanced disease status and poor prognosis. Because of the lack of targeting agents and limited therapeutic options, treatment of TNBC remains a great clinical challenge. Vasohibin 2 (VASH2) was previously identified as an angiogenic factor, but its role in TNBC tumorigenesis is unknown. Using quantitative PCR and western blot analyses, we found that VASH2 is overexpressed in TNBC cells and tissues. Knockdown of VASH2 via siRNA inhibited the proliferation of the TNBC cell lines b...
Source: American Journal of Translational Research - July 5, 2017 Category: Research Tags: Am J Transl Res Source Type: research
The effect of high Sox3 expression on lymphangiogenesis and lymph node metastasis in esophageal squamous cell carcinoma.
CONCLUSIONS: It is suggested that Sox3 possibly induces lymphangiogenesis by increasing the expression of VEGF-C/D in ESCC cells, thereby promoting the lymph node metastasis of the tumor. Thus, Sox-3 may become a new prognostic marker and therapeutic target in ESCC.
PMID: 28670361 [PubMed]
Source: American Journal of Translational Research - July 5, 2017 Category: Research Tags: Am J Transl Res Source Type: research
MicroRNA-93 inhibits apoptosis and promotes proliferation, invasion and migration of renal cell carcinoma ACHN cells via the TGF- β/Smad signaling pathway by targeting RUNX3.
In conclusion, miR-93 inhibits apoptosis and promotes proliferation, invasion, and migration of RCC cells via TGF-β/Smad signaling by inhibiting RUNX3.
PMID: 28804566 [PubMed]
Source: American Journal of Translational Research - August 15, 2017 Category: Research Tags: Am J Transl Res Source Type: research
A potential role for the Hippo pathway protein, YAP, in controlling proliferation, cell cycle progression, and autophagy in BCPAP and KI thyroid papillary carcinoma cells.
CONCLUSIONS: In papillary thyroid cancer YAP protein expression is positively correlated with the extent of TNM stage and positive lymph node metastasis. In thyroid cancer cell lines YAP appears to be important in stimulating cell proliferation while inhibiting autophagy.
PMID: 28804541 [PubMed]
Source: American Journal of Translational Research - August 15, 2017 Category: Research Tags: Am J Transl Res Source Type: research
MicroRNA-130a-3p suppresses cell viability, proliferation and invasion in nasopharyngeal carcinoma by inhibiting CXCL12.
In this study, we explored the molecular mechanisms of miR-130a-3p in inhibiting viability, proliferation, migration and invasion of NPC cells by suppressing CXCL12. The relative expression of miR-130a-3p and CXCL12 mRNA expression in tissues and cells was measured by qRT-PCR. NPC cell line CNE-2Z was transfected with miR-130a-3p mimics, CXCL12 siRNA, cDNA-CXCL12 and negative control. Western Blot was performed to detect CXCL12 expression. The MTT assay was performed to study cell viability. The colony formation assay was done to test cell growth. Flow cytometry was conducted to analyze cell cycle and apoptosis. The Transw...
Source: American Journal of Translational Research - September 3, 2017 Category: Research Tags: Am J Transl Res Source Type: research
