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Total 39 results found since Jan 2013.
Large-Scale Profiling of Kinase Dependencies in Cancer Cell Lines
Publication date: Available online 3 March 2016 Source:Cell Reports Author(s): James Campbell, Colm J. Ryan, Rachel Brough, Ilirjana Bajrami, Helen N. Pemberton, Irene Y. Chong, Sara Costa-Cabral, Jessica Frankum, Aditi Gulati, Harriet Holme, Rowan Miller, Sophie Postel-Vinay, Rumana Rafiq, Wenbin Wei, Chris T. Williamson, David A. Quigley, Joe Tym, Bissan Al-Lazikani, Timothy Fenton, Rachael Natrajan, Sandra J. Strauss, Alan Ashworth, Christopher J. Lord One approach to identifying cancer-specific vulnerabilities and therapeutic targets is to profile genetic dependencies in cancer cell line...
Source: Cell Reports - March 4, 2016 Category: Cytology Source Type: research
KDEL Receptors Assist Dengue Virus Exit from the Endoplasmic Reticulum
In this study, we demonstrate that dengue virus (DENV) interacts with KDEL receptors (KDELR), which cycle between the ER and Golgi apparatus, for vesicular transport from ER to Golgi. Depletion of KDELR by siRNA reduced egress of both DENV progeny and recombinant subviral particles (RSPs). Coimmunoprecipitation of KDELR with dengue structural protein prM required three positively charged residues at the N terminus, whose mutation disrupted protein interaction and inhibited RSP transport from the ER to the Golgi. Finally, siRNA depletion of class II Arfs, which results in KDELR accumulation in the Golgi, phenocopied result...
Source: Cell Reports - March 6, 2015 Category: Cytology Source Type: research
YAP Inhibition Restores Hepatocyte Differentiation in Advanced HCC, Leading to Tumor Regression
Publication date: Available online 12 March 2015 Source:Cell Reports Author(s): Julien Fitamant , Filippos Kottakis , Samira Benhamouche , Helen S. Tian , Nicolas Chuvin , Christine A. Parachoniak , Julia M. Nagle , Rushika M. Perera , Marjorie Lapouge , Vikram Deshpande , Andrew X. Zhu , Albert Lai , Bosun Min , Yujin Hoshida , Joseph Avruch , Daniela Sia , Genís Campreciós , Andrea I. McClatchey , Josep M. Llovet , David Morrissey , Lakshmi Raj , Nabeel Bardeesy Defective Hippo/YAP signaling in the liver results in tissue overgrowth and development of hepatocellular carcinoma (HCC). Here, we uncover mechanisms...
Source: Cell Reports - March 13, 2015 Category: Cytology Source Type: research
Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum
Publication date: Available online 18 August 2016 Source:Cell Reports Author(s): Keisuke Tabata, Masaru Arimoto, Masashi Arakawa, Atsuki Nara, Kazunobu Saito, Hiroko Omori, Arisa Arai, Tomohiro Ishikawa, Eiji Konishi, Ryosuke Suzuki, Yoshiharu Matsuura, Eiji Morita Flavivirus infection induces endoplasmic reticulum (ER) membrane rearrangements to generate a compartment for replication of the viral genome and assembly of viral particles. Using quantitative mass spectrometry, we identified several ESCRT (endosomal sorting complex required for transport) proteins that are recruited to sites of virus replication on the ER. S...
Source: Cell Reports - August 18, 2016 Category: Cytology Source Type: research
E3 Ligase RNF126 Directly Ubiquitinates Frataxin, Promoting Its Degradation: Identification of a Potential Therapeutic Target for Friedreich Ataxia
Publication date: 21 February 2017 Source:Cell Reports, Volume 18, Issue 8 Author(s): Monica Benini, Silvia Fortuni, Ivano Condò, Giulia Alfedi, Florence Malisan, Nicola Toschi, Dario Serio, Damiano Sergio Massaro, Gaetano Arcuri, Roberto Testi, Alessandra Rufini Friedreich ataxia (FRDA) is a severe genetic neurodegenerative disease caused by reduced expression of the mitochondrial protein frataxin. To date, there is no therapy to treat this condition. The amount of residual frataxin critically affects the severity of the disease; thus, attempts to restore physiological frataxin levels are considered therapeutically rele...
Source: Cell Reports - February 21, 2017 Category: Cytology Source Type: research
The Conserved RNA Binding Cyclophilin, Rct1, Regulates Small RNA Biogenesis and Splicing Independent of Heterochromatin Assembly
Publication date: 20 June 2017 Source:Cell Reports, Volume 19, Issue 12 Author(s): An-Yun Chang, Stephane E. Castel, Evan Ernst, Hyun Soo Kim, Robert A. Martienssen RNAi factors and their catalytic activities are essential for heterochromatin assembly in S. pombe. This has led to the idea that siRNAs can promote H3K9 methylation by recruiting the cryptic loci regulator complex (CLRC), also known as recombination in K complex (RIKC), to the nucleation site. The conserved RNA-binding protein Rct1 (AtCyp59/SIG-7) interacts with splicing factors and RNA polymerase II. Here we show that Rct1 promotes processing of pericentr...
Source: Cell Reports - June 21, 2017 Category: Cytology Source Type: research
Differential Effector Engagement by Oncogenic KRAS
Publication date: 13 February 2018 Source:Cell Reports, Volume 22, Issue 7 Author(s): Tina L. Yuan, Arnaud Amzallag, Rachel Bagni, Ming Yi, Shervin Afghani, William Burgan, Nicole Fer, Leslie A. Strathern, Katie Powell, Brian Smith, Andrew M. Waters, David Drubin, Ty Thomson, Rosy Liao, Patricia Greninger, Giovanna T. Stein, Ellen Murchie, Eliane Cortez, Regina K. Egan, Lauren Procter, Matthew Bess, Kwong Tai Cheng, Chih-Shia Lee, Liam Changwoo Lee, Christof Fellmann, Robert Stephens, Ji Luo, Scott W. Lowe, Cyril H. Benes, Frank McCormick KRAS can bind numerous effector proteins, which activate different downstream signal...
Source: Cell Reports - February 24, 2018 Category: Cytology Source Type: research
Diverse Regulators of Human Ribosome Biogenesis Discovered by Changes in Nucleolar Number
Publication date: 13 February 2018 Source:Cell Reports, Volume 22, Issue 7 Author(s): Katherine I. Farley-Barnes, Kathleen L. McCann, Lisa M. Ogawa, Janie Merkel, Yulia V. Surovtseva, Susan J. Baserga Ribosome biogenesis is a highly regulated, essential cellular process. Although studies in yeast have established some of the biological principles of ribosome biogenesis, many of the intricacies of its regulation in higher eukaryotes remain unknown. To understand how ribosome biogenesis is globally integrated in human cells, we conducted a genome-wide siRNA screen for regulators of nucleolar number. We found 139 proteins wh...
Source: Cell Reports - February 24, 2018 Category: Cytology Source Type: research
A PP6-ASK3 Module Coordinates the Bidirectional Cell Volume Regulation under Osmotic Stress
Publication date: 13 March 2018 Source:Cell Reports, Volume 22, Issue 11 Author(s): Kengo Watanabe, Tsuyoshi Umeda, Kuniyoshi Niwa, Isao Naguro, Hidenori Ichijo Cell volume regulation is a vital system for cellular activities. When perturbed by hypoosmotic or hyperosmotic stress, cells immediately induce the cell volume recovery system, regulatory volume decrease (RVD) or regulatory volume increase (RVI), respectively. In contrast to the knowledge about effector molecules, the molecular mechanisms linking osmosensing to RVD/RVI induction remain unknown. Additionally, few reciprocal responders in the bidirectional osmotic ...
Source: Cell Reports - March 14, 2018 Category: Cytology Source Type: research
FXR1 Is an IL-19-Responsive RNA-Binding Protein that Destabilizes Pro-inflammatory Transcripts in Vascular Smooth Muscle Cells
Publication date: 31 July 2018Source: Cell Reports, Volume 24, Issue 5Author(s): Allison B. Herman, Christine N. Vrakas, Mitali Ray, Sheri E. Kelemen, Michael J. Sweredoski, Annie Moradian, Dale S. Haines, Michael V. AutieriSummaryThis work identifies the fragile-X-related protein (FXR1) as a reciprocal regulator of HuR target transcripts in vascular smooth muscle cells (VSMCs). FXR1 was identified as an HuR-interacting protein by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The HuR-FXR1 interaction is abrogated in RNase-treated extracts, indicating that their association is tethered by mRNAs. FXR1 expression...
Source: Cell Reports - August 1, 2018 Category: Cytology Source Type: research
Genome-wide RNAi Screen Reveals a Role for Multipass Membrane Proteins in Endosome-to-Golgi Retrieval
Publication date: 11 December 2014 Source:Cell Reports, Volume 9, Issue 5 Author(s): Sophia Y. Breusegem , Matthew N.J. Seaman Endosome-to-Golgi retrieval is an essential membrane trafficking pathway required for many important physiological processes and linked to neurodegenerative disease and infection by bacterial and viral pathogens. The prototypical cargo protein for this pathway is the cation-independent mannose 6-phosphate receptor (CIMPR), which delivers lysosomal hydrolases to endosomes. Efficient retrieval of CIMPR to the Golgi requires the retromer complex, but other aspects of the endosome-to-Golgi retrieva...
Source: Cell Reports - December 12, 2014 Category: Cytology Source Type: research
In Vivo RNAi Screening Identifies MDA5 as a Significant Contributor to the Cellular Defense against Influenza A Virus
Publication date: Available online 11 June 2015 Source:Cell Reports Author(s): Asiel A. Benitez , Maryline Panis , Jia Xue , Andrew Varble , Jaehee V. Shim , Amy L. Frick , Carolina B. López , David Sachs , Benjamin R. tenOever Responding to an influenza A virus (IAV) infection demands an effective intrinsic cellular defense strategy to slow replication. To identify contributing host factors to this defense, we exploited the host microRNA pathway to perform an in vivo RNAi screen. To this end, IAV, lacking a functional NS1 antagonist, was engineered to encode individual siRNAs against antiviral host genes in an ef...
Source: Cell Reports - June 12, 2015 Category: Cytology Source Type: research
Defined MicroRNAs Induce Aspects of Maturation in Mouse and Human Embryonic-Stem-Cell-Derived Cardiomyocytes
Publication date: Available online 10 September 2015 Source:Cell Reports Author(s): Desy S. Lee, Jyh-Hong Chen, David J. Lundy, Chung-Hung Liu, Shiaw-Min Hwang, Lil Pabon, Ru-Chi Shieh, Chien-Chang Chen, Sheng-Nan Wu, Yu-Ting Yan, Sho-Tone Lee, Po-Min Chiang, Shu Chien, Charles E. Murry, Patrick C.H. Hsieh Pluripotent-cell-derived cardiomyocytes have great potential for use in research and medicine, but limitations in their maturity currently constrain their usefulness. Here, we report a method for improving features of maturation in murine and human embryonic-stem-cell-derived cardiomyocytes (m/hESC-C...
Source: Cell Reports - September 11, 2015 Category: Cytology Source Type: research
Age-Associated Methylation Suppresses SPRY1, Leading to a Failure of Re-quiescence and Loss of the Reserve Stem Cell Pool in Elderly Muscle
Publication date: Available online 29 October 2015 Source:Cell Reports Author(s): Anne Bigot, William J. Duddy, Zamalou G. Ouandaogo, Elisa Negroni, Virginie Mariot, Svetlana Ghimbovschi, Brennan Harmon, Aurore Wielgosik, Camille Loiseau, Joe Devaney, Julie Dumonceaux, Gillian Butler-Browne, Vincent Mouly, Stéphanie Duguez The molecular mechanisms by which aging affects stem cell number and function are poorly understood. Murine data have implicated cellular senescence in the loss of muscle stem cells with aging. Here, using human cells and by carrying out experiments within a strictly pre-senescent divi...
Source: Cell Reports - October 31, 2015 Category: Cytology Source Type: research
Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells
Publication date: Available online 6 December 2015 Source:Cell Reports Author(s): Katarzyna A. Makowska, Ruth E. Hughes, Kathryn J. White, Claire M. Wells, Michelle Peckham We investigated the myosin expression profile in prostate cancer cell lines and found that Myo1b, Myo9b, Myo10, and Myo18a were expressed at higher levels in cells with high metastatic potential. Moreover, Myo1b and Myo10 were expressed at higher levels in metastatic tumors. Using an siRNA-based approach, we found that knockdown of each myosin resulted in distinct phenotypes. Myo10 knockdown ablated filopodia and decreased 2D migration speed....
Source: Cell Reports - December 7, 2015 Category: Cytology Source Type: research
