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A Role for the Insulin Receptor in the Suppression of Dengue Virus and Zika Virus in Wolbachia-Infected Mosquito Cells
This study identifies Wolbachia-mediated downregulation of insulin receptor kinase activity as a mechanism contributing to the blocking of super-infection by arboviruses.Graphical Abstract
Source: Cell Reports - January 16, 2019 Category: Cytology Source Type: research

The Host Protein Reticulon 3.1A Is Utilized by Flaviviruses to Facilitate Membrane Remodelling
In this study, we identified a key role for the membrane-bending host protein Reticulon 3.1 (RTN3.1A) during the replication cycle of three flaviviruses: West Nile virus (WNV), Dengue virus (DENV), and Zika virus (ZIKV). We observed that, during infection, RTN3.1A is redistributed and recruited to the viral replication complex, a recruitment facilitated via the WNV NS4A protein, however, not DENV or ZIKV NS4A. Critically, small interfering RNA (siRNA)-mediated knockdown of RTN3.1A expression attenuated WNV, DENV, and ZIKV replication and severely affected the stability and abundance of the NS4A protein, coinciding with a s...
Source: Cell Reports - November 7, 2017 Category: Cytology Source Type: research

Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum
Publication date: Available online 18 August 2016 Source:Cell Reports Author(s): Keisuke Tabata, Masaru Arimoto, Masashi Arakawa, Atsuki Nara, Kazunobu Saito, Hiroko Omori, Arisa Arai, Tomohiro Ishikawa, Eiji Konishi, Ryosuke Suzuki, Yoshiharu Matsuura, Eiji Morita Flavivirus infection induces endoplasmic reticulum (ER) membrane rearrangements to generate a compartment for replication of the viral genome and assembly of viral particles. Using quantitative mass spectrometry, we identified several ESCRT (endosomal sorting complex required for transport) proteins that are recruited to sites of virus replication on the ER. S...
Source: Cell Reports - August 18, 2016 Category: Cytology Source Type: research

KDEL Receptors Assist Dengue Virus Exit from the Endoplasmic Reticulum
In this study, we demonstrate that dengue virus (DENV) interacts with KDEL receptors (KDELR), which cycle between the ER and Golgi apparatus, for vesicular transport from ER to Golgi. Depletion of KDELR by siRNA reduced egress of both DENV progeny and recombinant subviral particles (RSPs). Coimmunoprecipitation of KDELR with dengue structural protein prM required three positively charged residues at the N terminus, whose mutation disrupted protein interaction and inhibited RSP transport from the ER to the Golgi. Finally, siRNA depletion of class II Arfs, which results in KDELR accumulation in the Golgi, phenocopied result...
Source: Cell Reports - March 6, 2015 Category: Cytology Source Type: research