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Role of PUMA in methamphetamine-induced neuronal apoptosis.
This study aimed to examine the effects of PUMA in METH-induced neuronal apoptosis. We determined PUMA protein expression in PC12 cells and SH-SY5Y cells after METH exposure using western blot. We also observed the effect of METH on neuronal apoptosis after silencing PUMA expression with siRNA using TUNEL staining and flow cytometry. Additionally, to investigate possible mechanisms of METH-induced PUMA-mediated neuronal apoptosis, we measured the protein expression of apoptotic markers, including cleaved caspase-3, cleaved PARP, Bax, B-cell leukemia/lymphoma-2 (Bcl-2) and cytochrome c (cyto c), after METH treatment with or...
Source: Toxicology Letters - October 30, 2015 Category: Toxicology Authors: Chen C, Qincao L, Xu J, Du S, Huang E, Liu C, Lin Z, Xie WB, Wang H Tags: Toxicol Lett Source Type: research