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CYP27A1 inhibits proliferation and migration of clear cell renal cell carcinoma via activation of LXRs/ABCA1
Exp Cell Res. 2022 Jul 7:113279. doi: 10.1016/j.yexcr.2022.113279. Online ahead of print.ABSTRACTCholesterol homeostasis plays an important role in the maintenance of normal body functions. CYP27A1 is a key enzyme known to regulate cholesterol homeostasis, which catalyzes the conversion of cholesterol to 27-HC and has been implicated in the occurrence and metastasis of various cancer types. The present study aimed to explore the regulatory role of CYP27A1 in the development of clear cell renal cell carcinoma (ccRCC). In particular, the effect of CYP27A1 on the proliferation and migration of ccRCC cells was investigated. Th...
Source: Experimental Cell Research - July 10, 2022 Category: Cytology Authors: Zhijuan Liang Wei Jiao Liping Wang Yuanbin Chen Dan Li Zhao Zhang Zhilei Zhang Ye Liang Haitao Niu Source Type: research

Rab22a promotes the proliferation, migration, and invasion of lung adenocarcinoma via up-regulating PI3K/Akt/mTOR signaling pathway
Exp Cell Res. 2022 Apr 26:113179. doi: 10.1016/j.yexcr.2022.113179. Online ahead of print.ABSTRACTRab22a, a member of the proto-oncogene RAS family, belongs to the Rab5 subfamily. It participates in early endosome formation and regulates vesicle trafficking. The relationship between Rab22a and tumorigenesis remains elusive. In non-small cell lung cancer specimens, immunohistochemical staining showed consistently high expression of Rab22a in lung adenocarcinoma, but not in squamous cell carcinoma. In lung adenocarcinoma cell lines, A549 and H1299, transfection with Rab22a significantly promoted cell proliferation, migration...
Source: Experimental Cell Research - April 29, 2022 Category: Cytology Authors: Jinping Wang Xue Luo Jinxi Lu Xi Wang Yuan Miao Qingchang Li Liang Wang Source Type: research

High expression of Notch2 drives tongue squamous cell carcinoma carcinogenesis.
Abstract Tongue squamous cell carcinoma (TSCC) is one of the most common cancers in the oral cavity. Notch signaling is frequently dysregulated in cancer. However, the role of Notch2 in TSCC is not well understood. The aim of this study was to investigate the effect of abnormal expression of Notch2 in TSCC. The expression of Notch2 was tested in 47 pairs of tissues from tongue cancer and normal samples by using immunohistochemical staining. Tongue cancer cells were transfected with siRNA or plasmid. The proliferation of the cells was tested by the CCK8 assay and colony formation assay. Subcutaneous tumor model was...
Source: Experimental Cell Research - December 28, 2020 Category: Cytology Authors: Gan RH, Lin LS, Zheng DP, Zhao Y, Ding LC, Zheng DL, Lu YG Tags: Exp Cell Res Source Type: research

Prognostic and therapeutic relevance of phosphofructokinase platelet-type (PFKP) in breast cancer.
Abstract In the present study, we have explored the prognostic value of the Phosphofructokinase Platelet-type (PFKP) expression and its therapeutic relevance in metastatic breast cancer. PFKP immunohistochemistry was performed on Invasive ductal carcinomas (IDCs; n = 87) of breast, and its association with clinicopathological parameters were evaluated. Using online meta-analysis tools, PFKP's prognostic value was investigated in overall breast cancer as well as in triple negative subtype (TNBCs). For in vitro analysis, MDA-MB-231 cells model was used in order to elucidate mechanisms behind PFKP regulated gly...
Source: Experimental Cell Research - September 9, 2020 Category: Cytology Authors: Umar SM, Kashyap A, Kahol S, Mathur S, Gogia A, Deo SVS, Prasad CP Tags: Exp Cell Res Source Type: research

miR-194 Inhibits Liver Cancer Stem Cell Expansion by Regulating RAC1 pathway.
Abstract Liver cancer stem cells (CSCs) contribute to tumorigenesis, progression, drug resistance and recurrence of hepatocellular carcinoma (HCC). However, the underlying mechanism for the propagation of liver CSCs remains unclear. Herein, we observed low expression of miR-194 in chemoresistant HCC cells. A remarkable decrease of miR-194 was detected in EpCAM or CD133-positive liver CSCs and CSC-enriched hepatoma spheres. Interference miR-194 facilitated liver CSCs expansion by enhancing the self-renewal of liver CSCs. While up-regulating miR-194 inhibited liver CSCs expansion by suppressing the self-renewal of l...
Source: Experimental Cell Research - March 3, 2019 Category: Cytology Authors: Ran RZ, Chen J, Cui LJ, Lin XL, Fan MM, Cong ZZ, Zhang H, Tan WF, Zhang QG, Zhang YJ Tags: Exp Cell Res Source Type: research

MicroRNA-98 promotes drug resistance and regulates mitochondrial dynamics by targeting LASS2 in bladder cancer cells.
In conclusion, our study demonstrates that miR-98 targets LASS2 and regulates bladder cancer chemoresistance through modulation of mitochondrial function. PMID: 30463687 [PubMed - in process]
Source: Experimental Cell Research - November 23, 2018 Category: Cytology Authors: Luan T, Fu S, Huang L, Zuo Y, Ding M, Li N, Chen J, Wang H, Wang J Tags: Exp Cell Res Source Type: research

ARHGEF10L Contributes to Liver Tumorigenesis through RhoA-ROCK1 Signaling and the Epithelial-Mesenchymal Transition.
This study used the Illumina GoldenGate microassay, Sequenom MassARRAY and TaqMan to analyze possible correlations between tag single nucleotide polymorphisms (tag SNPs) in the ARHGEF10L locus and various tumor risks. The genotyping analyses demonstrated a strong association of rs2244444 and rs12732894 with liver cancer. Western blotting and immunohistochemistry also revealed increased expression of ARHGEF10L in hepatocellular carcinoma tissues. Furthermore, increased cell proliferation, cell migration and RhoA activity; increased expression of Rho-associated coiled-coil kinase-1 (ROCK1), phospho- Ezrin/Radixin/Moesin (ERM...
Source: Experimental Cell Research - November 13, 2018 Category: Cytology Authors: Tang J, Liu C, Xu B, Wang D, Ma Z, Chang X Tags: Exp Cell Res Source Type: research

Antihypertensive drug-candesartan attenuates TRAIL resistance in human lung cancer via AMPK-mediated inhibition of autophagy flux.
Abstract Angiotensin II type 1 receptor blockers (ARBs) are widely used as antihypertensive drugs. Candesartan is an ARB that has also been known for its anticancer effects but the exact molecular mechanism is remaining elusive. In this research, we showed for the first time that candesartan treatment significantly sensitized human lung adenocarcinoma cells to Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis by targeting TRAIL-DR5. TRAIL selectively kills cancer cells by binding to death receptors on the cell membrane, beyond the levels causing minimal toxicity in normal cells. Ho...
Source: Experimental Cell Research - April 22, 2018 Category: Cytology Authors: Rasheduzzaman M, Park SY Tags: Exp Cell Res Source Type: research

Salinomycin acts through reducing AKT-dependent thymidylate synthase expression to enhance erlotinib-induced cytotoxicity in human lung cancer cells.
In this study, we showed that erlotinib (1.25 - 10μM) treatment down-regulating of TS expression in an AKT inactivation manner in two NSCLC cell lines, human lung squamous cell carcinoma H1703 and adenocarcinoma H1975 cells. Knockdown of TS using small interfering RNA (siRNA) or inhibiting AKT activity with PI3K inhibitor LY294002 enhanced the cytotoxicity and cell growth inhibition of erlotinib. A combination of erlotinib and salinomycin resulted in synergistic enhancement of cytotoxicity and cell growth inhibition in NSCLC cells, accompanied with reduced protein levels of phospho-AKT(Ser473), phospho-AKT(Thr308), and TS...
Source: Experimental Cell Research - April 25, 2017 Category: Cytology Authors: Tung CL, Chen JC, Wu CH, Peng YS, Chen WC, Zheng HY, Jian YJ, Wei CL, Cheng YT, Lin YW Tags: Exp Cell Res Source Type: research

Integrin signaling potentiates transforming growth factor-beta 1 (TGF- β1) dependent down-regulation of E-Cadherin expression - important implications for Epithelial to Mesenchymal Transition (EMT) in Renal Cell Carcinoma.
Integrin signaling potentiates transforming growth factor-beta 1 (TGF-β1) dependent down-regulation of E-Cadherin expression - important implications for Epithelial to Mesenchymal Transition (EMT) in Renal Cell Carcinoma. Exp Cell Res. 2017 Mar 28;: Authors: Feldkoren B, Hutchinson R, Rapaport Y, Mahajan A, Margulis V Abstract Signal transduction through the transforming growth factor-beta 1 (TGF-β1) pathway affects epithelial to mesenchymal transition (EMT), partly by modulation of E-Cadherin expression. The concurrent impact of extracellular matrix driven regulation of integrin signaling on EMT ha...
Source: Experimental Cell Research - March 28, 2017 Category: Cytology Authors: Feldkoren B, Hutchinson R, Rapaport Y, Mahajan A, Margulis V Tags: Exp Cell Res Source Type: research

NOR1 promotes hepatocellular carcinoma cell proliferation and migration through modulating the Notch signaling pathway.
In conclusion, these results indicate that NOR1 may be involved in the progression of HCC and thus may be a potential target for the treatment of liver cancer. PMID: 28232113 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - February 19, 2017 Category: Cytology Authors: You K, Sun P, Yue Z, Li J, Xiong W, Wang J Tags: Exp Cell Res Source Type: research

Experimental evidence of Migfilin as a new therapeutic target of hepatocellular carcinoma metastasis.
Abstract Migfilin is a novel cell-matrix adhesion protein known to interact with Vasodilator Stimulated Phosphoprotein (VASP) and be localized both at cell-matrix and cell-cell adhesions. To date there is nothing known on its role in hepatocellular carcinoma (HCC). As matrix is important in metastasis, we aimed to investigate the Migfilin's role in HCC metastasis using two human HCC cell lines that differ in their metastatic potential; non-invasive Alexander cells and the highly invasive HepG2 cells. We silenced Migfilin by siRNA and studied its effect on signaling and metastasis-related cellular properties. We sh...
Source: Experimental Cell Research - March 12, 2015 Category: Cytology Authors: Gkretsi V, Bogdanos DP Tags: Exp Cell Res Source Type: research

Dicoumarol sensitizes renal cell carcinoma Caki cells to TRAIL-induced apoptosis through down-regulation of Bcl-2, Mcl-1 and c-FLIP in a NQO1-independent manner.
Abstract In the study, we investigated the effect of dicoumarol, an anti-coagulant agent with the inhibitory activity of NAD(P)H quinone oxidoreductase 1 (NQO1), on TRAIL-induced apoptosis in renal cancer cell. Combined treatment with dicoumarol and TRAIL significantly induced apoptosis in various human renal carcinoma cells including Caki, ACHN, and A498, but not in normal human skin fibroblasts (HSF) and mouse kidney cells (TMCK-1). When we elucidated the relevance of NQO1 in dicoumarol plus TRAIL-mediated apoptosis, both ES936 (a NQO1 inhibitor) and knockdown of NQO1 with siRNA had no effect on TRAIL-mediated a...
Source: Experimental Cell Research - January 22, 2014 Category: Cytology Authors: Park EJ, Min KJ, Sook Choi K, Kwon TK Tags: Exp Cell Res Source Type: research

Role of the EphB2 receptor in autophagy, apoptosis and invasion in human breast cancer cells.
Abstract The Eph and Ephrin proteins, which constitute the largest family of receptor tyrosine kinases, are involved in normal tissue development and cancer progression. Here, we examined the expression and role of the B-type Eph receptor EphB2 in breast cancers. By immunohistochemistry using a progression tissue microarray of human clinical samples, we found EphB2 to be expressed in benign tissues, but strongly increased in cancers particularly in invasive and metastatic carcinomas. Subsequently, we found evidence that EphB2, whose expression varies in established cell breast lines, possesses multiple functions. ...
Source: Experimental Cell Research - November 6, 2013 Category: Cytology Authors: Chukkapalli S, Amessou M, Kumar Dilly A, Dekhil H, Zhao J, Liu Q, Bejna A, Thomas RD, Bandyopadhyay S, Bismar TA, Neill D, Azoulay L, Batist G, Kandouz M Tags: Exp Cell Res Source Type: research