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MicroRNA-488-3p sensitizes malignant melanoma cells to cisplatin by targeting PRKDC.
In this study, we demonstrated that miR-488-3p is significantly down regulated in MM clinical specimens and cell lines. Ectopic expression of miR-488-3p resulted in markedly increased drug sensitivity of MM cells in vitro and in vivo. The DNA-activated, catalytic polypeptide (PRKDC), which encodes DNA-dependent protein kinase catalytic subunit (DNA-PKcs), was identified as a direct target of miR-488-3p using luciferase reporter assays, qRT-PCR and western blotting analyses. PRKDC knockdown by small interfering RNA (siRNA) alone promoted sensitivity of MM cells to cisplatin (DDP) while overexpression of PRKDC partially resc...
Source: Cell Biology International - March 22, 2017 Category: Cytology Authors: Li N, Ma Y, Ma L, Guan Y, Ma L, Yang D Tags: Cell Biol Int Source Type: research

Knockdown of tumor protein D52-like 2 induces cell growth inhibition and apoptosis in oral squamous cell carcinoma.
Abstract Tumor protein D52-like 2 (TPD52L2) and its family members form homo- and heteromeric complexes essential for cell proliferation in multiple human cancers. TPD52L2 is involved in cell migration and attachment in oral squamous cell carcinoma (OSCC). To confirm the role of TPD52L2 in OSCC, we employed the lentivirusdelivered small interfering RNA (siRNA) technique to knock down TPD52L2 expression in 2 OSCC cell lines, CAL27 and KB. Knockdown of TPD52L2 by RNA interference markedly suppressed cell proliferation and colony formation. Cell cycle analysis showed that depletion of TPD52L2 led to CAL27 cells arres...
Source: Cell Biology International - September 27, 2014 Category: Cytology Authors: He Y, Chen F, Cai Y, Chen S Tags: Cell Biol Int Source Type: research

RNAi-mediated MMP-9 silencing inhibits mouse melanoma cell invasion and migration in vitro and in vivo.
Abstract MMP-9 participates in tumour growth, invasion, metastasis and vascularisation. Thus, inhibition of MMP-9 may be involved in the process of tumourigenesis. We have investigated the effect of RNAi-mediated MMP-9 silencing on inhibiting invasion and migration of mouse melanoma cell B16. A specific and optimised siRNA vector was used to target MMP-9 mRNA synthesis in B16 cells. Changes of invasion and migration capability of B16 cell were examined after transfection at different time, and a footpad tumour model was performed to measure the effect of MMP-9 siRNA on melanoma tumourigenesis in vivo. In vitro, do...
Source: Cell Biology International - March 30, 2013 Category: Cytology Authors: Tang ZY, Liu Y, Liu LX, Ding XY, Zhang H, Fang LQ Tags: Cell Biol Int Source Type: research