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Influence of glutathione-S-transferase (GST) inhibition on lung epithelial cell injury: role of oxidative stress and metabolism
Oxidant-mediated tissue injury is key to the pathogenesis of acute lung injury. Glutathione-S-transferases (GSTs) are important detoxifying enzymes that catalyze the conjugation of glutathione with toxic oxidant compounds and are associated with acute and chronic inflammatory lung diseases. We hypothesized that attenuation of cellular GST enzymes would augment intracellular oxidative and metabolic stress and induce lung cell injury. Treatment of murine lung epithelial cells with GST inhibitors, ethacrynic acid (EA), and caffeic acid compromised lung epithelial cell viability in a concentration-dependent manner. These inhib...
Source: AJP: Lung Cellular and Molecular Physiology - June 15, 2015 Category: Respiratory Medicine Authors: Fletcher, M. E., Boshier, P. R., Wakabayashi, K., Keun, H. C., Smolenski, R. T., Kirkham, P. A., Adcock, I. M., Barton, P. J., Takata, M., Marczin, N. Tags: ARTICLES Source Type: research

Nox2/ROS-dependent human antigen R translocation contributes to TNF-{alpha}-induced SOCS-3 expression in human tracheal smooth muscle cells
Elevated levels of TNF-α have been detected in the airway fluids, which may induce upregulation of inflammatory proteins. Suppressors of cytokine signaling (SOCS)-3 proteins can be induced by various cytokines and negatively regulated inflammatory responses. Although TNF-α has been shown to induce SOCS-3 expression, the mechanisms underlying TNF-α-induced SOCS-3 expression in human tracheal smooth muscle cells (HTSMCs) remain unclear. Here, we showed that TNF-α induced SOCS-3 expression, which was inhibited by pretreatment with the inhibitor of transcription level (actinomycin D), translation level ...
Source: AJP: Lung Cellular and Molecular Physiology - March 15, 2014 Category: Respiratory Medicine Authors: Hsu, C.-K., Lee, I.-T., Lin, C.-C., Hsiao, L.-D., Yang, C.-M. Tags: ARTICLES Source Type: research