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Aurin Causes Lethal Proteotoxic Stress in Melanoma Cells Signal Transduction
Pharmacological induction of proteotoxic stress is rapidly emerging as a promising strategy for cancer cell-directed chemotherapeutic intervention. Here, we describe the identification of a novel drug-like heat shock response inducer for the therapeutic induction of proteotoxic stress targeting malignant human melanoma cells. Screening a focused library of compounds containing redox-directed electrophilic pharmacophores employing the Stress 4-[bis(p-hydroxyphenyl)methylene]-2,5-cyclohexadien-1-one) was identified as an experimental cell stress modulator that causes (i) heat shock factor transcriptional activation, (ii) up-...
Source: Journal of Biological Chemistry - January 16, 2015 Category: Chemistry Authors: Davis, A. L., Qiao, S., Lesson, J. L., Rojo de la Vega, M., Park, S. L., Seanez, C. M., Gokhale, V., Cabello, C. M., Wondrak, G. T. Tags: Cell Biology Source Type: research

Regulation of ovulatory genes in bovine granulosa cells: lessons from siRNA silencing of PTGS2
In this study, we compared the induction of these ovulatory genes in bovine granulosa cells (GCs) in vivo (after LH surge) and in vitro (forskolin (FRS) treatment). These genes were markedly stimulated in GCs isolated from cows 21 h after LH-surge. In isolated GCs, FRS induced a distinct temporal profile for each gene. Generally, there was a good agreement between the in vivo and in vitro inductions of these genes except for PTX3. Lack of PTX3 induction in isolated GCs culture suggests that other follicular compartments may mediate its induction by LH. Next, to study the role of PTGS2 and prostaglandins (PGs) in the cascad...
Source: Reproduction - November 28, 2014 Category: Reproduction Medicine Authors: Shrestha, K., Lukasik, K., Baufeld, A., Vanselow, J., Moallem, U., Meidan, R. Tags: Research Source Type: research

REGULATION OF OVULATORY GENES IN BOVINE GRANULOSA CELLS: LESSONS FROM siRNA SILENCING OF PTGS2.
Abstract Prostaglandin endoperoxide synthase 2 (PTGS2), tumor necrosis factor-alpha-induced protein-6 (TNFAIP6), pentraxin-3 (PTX3), epidermal growth factor like factors: amphiregulin (AREG) and epiregulin (EREG) are essential for successful ovulation. We compared here the induction of these ovulatory genes in bovine granulosa cells (GCs) in vivo (after LH surge) and in vitro (forskolin treatment). These genes were markedly stimulated in GCs isolated from cows 21hr after LH- surge. In isolated GCs, forskolin induced a distinct temporal profile for each gene. Generally, there was a good agreement between the in viv...
Source: Reproduction - October 16, 2014 Category: Reproduction Medicine Authors: Shrestha K, Lukasik K, Baufeld A, Vanselow J, Moallem U, Meidan R Tags: Reproduction Source Type: research

Lymphoblastoid Cell Lines Models of Drug Response: Successes and Lessons from This Pharmacogenomic Model.
Abstract A new standard for medicine is emerging that aims to improve individual drug responses through studying associations with genetic variations. This field, pharmacogenomics, is undergoing a rapid expansion due to a variety of technological advancements that are enabling higher throughput with reductions in cost. Here we review the advantages, limitations, and opportunities for using lymphoblastoid cell lines (LCL) as a model system for human pharmacogenomic studies. There are a wide range of publicly available resources with genome-wide data available for LCLs from both related and unrelated populations, re...
Source: Current Molecular Medicine - August 11, 2014 Category: Molecular Biology Authors: Jack J, Rotroff D, Motsinger-Reif AA Tags: Curr Mol Med Source Type: research

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