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Viruses, Vol. 9, Pages 257: Engineered Disease Resistance in Cotton Using RNA-Interference to Knock down Cotton leaf curl Kokhran virus-Burewala and Cotton leaf curl Multan betasatellite Expression
In this study, a small interfering RNA (siRNA) construct was designed to target the AC1 gene of Cotton leaf curl Kokhran virus-Burewala (CLCuKoV-Bu) and the βC1 gene and satellite conserved region of the Cotton leaf curl Multan betasatellite (CLCuMB). The AC1 gene and CLCuMB coding and non-coding regions function in replication initiation and suppression of the plant host defense pathway, respectively. The construct, Vβ, was transformed into cotton plants using the Agrobacterium-mediated embryo shoot apex cut method. Results from fluorescence in situ hybridization and karyotyping assays indicated that six of the 11 T1 pl...
Source: Viruses - September 14, 2017 Category: Virology Authors: Aftab Ahmad Muhammad Zia-Ur-Rehman Usman Hameed Abdul Qayyum Rao Ammara Ahad Aneela Yasmeen Faheem Akram Kamran Bajwa Jodi Scheffler Idrees Nasir Ahmad Shahid Muhammad Iqbal Tayyab Husnain Muhammad Haider Judith Brown Tags: Article Source Type: research