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Generation of transgenic pigs carrying siRNA vector directed against human tissue factor expression
ConclusionsThe AVR is currently the bottleneck in porcine‐to‐primate organ xenotransplantation. Controlling TF‐induced coagulation might provide protective effects for porcine xenografts. Thus, TF knockdown pigs could be critical in producing the ultimate multi‐transgenic pig as organ donor for human patients. References[1] Toomey J, Kratzer K, Lasky N, Stanton J, Broze GJ. Targeted disruption of the murine tissue factor gene results in embryonic lethality. Blood. 1996; 88(5): 1583–1587.
Source: Xenotransplantation - April 19, 2014 Category: Transplant Surgery Authors: Hellen Ahrens, Björn Petersen, Doris Herrmann, Andrea Lucas‐Hahn, Petra Hassel, Maren Ziegler, Wilfried Kues, Heiner Niemann Tags: Program and Abstracts Source Type: research

Zink finger nucleases and siRNAs: use in transgenic pig production for xenotransplantation
Emerging technologies, including the use of specific nucleases, transposons and specific siRNAs will bring significant improvements with regard to efficiency, precision and safety of gene transfer. Here, we summarize recent findings from our laboratory on the integration of Zinc‐finger nuclease technology (ZFNs) and specific siRNAs into a research programme towards the development and characterization of multi‐transgenic pigs suitable for xenotransplantation. Zinc‐finger nucleases (ZFNs) are powerful tools for producing gene knockouts (KOs) with very high efficiency. ZFNs were used to induce a biallelic knockout of t...
Source: Xenotransplantation - February 5, 2013 Category: Transplant Surgery Authors: Heiner Niemann, Björn Petersen, Hellen Ahrens, Wilfried Kues Source Type: research