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Antibacterial effect of mesenchymal stem cells against Escherichia coli is mediated by secretion of beta‐defensin‐2 via toll like receptor 4 signaling
This study was done to elucidate the mechanism underlying the antibacterial effects of MSCs. The growth of E. coli in vitro was significantly inhibited only by MSCs or their conditioned medium with bacterial preconditioning, but not by fibroblasts or their conditioned medium. Microarray analysis identified significant up‐regulation of toll like receptors (TLR)‐2 and TLR‐4, and β‐defensin 2 (BD2) in MSCs compared with fibroblasts after E. coli exposure. The increased BD2 level and the in vitro antibacterial effects of MSCs were abolished by specific antagonist or by siRNA‐mediated knockdown of TLR‐4, but not TL...
Source: Cellular Microbiology - September 1, 2015 Category: Microbiology Authors: Dong Kyung Sung, Yun Sil Chang, Se In Sung, Hye Soo Yoo, So Yoon Ahn, Won Soon Park Tags: Research article Source Type: research

Klebsiella pneumoniae targets an EGF receptor‐dependent pathway to subvert inflammation
Summary The NF‐κB transcriptional factor plays a key role governing the activation of immune responses. Klebsiella pneumoniae is an important cause of community‐acquired and nosocomial pneumonia. Evidence indicates that K. pneumoniae infections are characterized by lacking an early inflammatory response. Recently, we have demonstrated that Klebsiella antagonizes the activation of NF‐κB via the deubiquitinase CYLD. In this work, by applying a high‐throughput siRNA gain‐of‐function screen interrogating the human kinome, we identified 17 kinases that when targeted by siRNA restored IL‐1β‐dependent NF‐κ...
Source: Cellular Microbiology - February 17, 2013 Category: Microbiology Authors: Christian G. Frank, Verónica Reguerio, Marion Rother, David Moranta, André P. Maeurer, Junkal Garmendia, Thomas F. Meyer, José A. Bengoechea Tags: Research Article Source Type: research

Klebsiella pneumoniae targets an EGF receptor‐dependent pathway to subvert inflammation.
Abstract The NF‐κB transcriptional factor plays a key role governing the activation of immune responses. Klebsiella pneumoniae is an important cause of community‐acquired and nosocomial pneumonia. Evidence indicates that K. pneumoniae infections are characterized by lacking an early inflammatory response. Recently, we have demonstrated that Klebsiella antagonizes the activation of NF‐κB via the deubiquitinase CYLD. In this work, by applying a high‐throughput siRNA gain‐on‐function screen interrogating the human kinome, we identified 17 kinases that when targeted by siRNA restored IL‐1β‐dependent NF‐κB...
Source: Cellular Microbiology - January 25, 2013 Category: Microbiology Authors: Christian G. Frank, Verónica Reguerio, Marion Rother, David Moranta, André P. Maeurer, Junkal Garmendia, Thomas F. Meyer, José A. Bengoechea Tags: Research Article Source Type: research