• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Reduced DPP4 activity improves insulin signaling in primary human adipocytes.
Abstract DPP4 is a ubiquitously expressed cell surface protease which is also released to the circulation as soluble DPP4 (sDPP4). Recently, we identified DPP4 as a novel adipokine oversecreted in obesity and thus potentially linking obesity to the metabolic syndrome. Furthermore, sDPP4 impairs insulin signaling in an autocrine and paracrine fashion in different cell types. However, it is still unknown which functional role DPP4 might play in adipocytes. Therefore, primary human adipocytes were treated with a specific DPP4 siRNA. Adipocyte differentiation was not affected by DPP4 silencing. Interestingly, DPP4 red...
Source: Biochemical and Biophysical Research communications - February 9, 2016 Category: Biochemistry Authors: Röhrborn D, Brückner J, Sell H, Eckel J Tags: Biochem Biophys Res Commun Source Type: research

Resistin regulates the expression of plasminogen activator inhibitor-1 in 3T3-L1 adipocytes.
In this study, to clarify the relationship between expression of resistin and PAI-1, PAI-1 expression in differentiated 3T3-L1 adipocytes was measured after transfection with anti-resistin siRNA. We found that PAI-1 gene expression and secreted PAI-1 protein were significantly decreased by resistin knockdown. Furthermore, phosphorylation of Akt, which can inhibit PAI-1 expression, was accelerated and the activity of protein phosphatase 2A (PP2A) was suppressed in resistin knockdown 3T3-L1 adipocytes. In addition, the expression of glucose transporter type 4, a ChREBP target gene, was reduced and was associated with inhibit...
Source: Biochemical and Biophysical Research communications - March 22, 2014 Category: Biochemistry Authors: Ikeda Y, Tsuchiya H, Hama S, Kajimoto K, Kogure K Tags: Biochem Biophys Res Commun Source Type: research