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Inhibition of AIF-1 alleviates laser-induced macular neovascularization by inhibiting endothelial cell proliferation via restrained p44/42 MAPK signaling pathway
This study aimed to understand how AIF-1 effects the proliferation of CECs and the subsequent progression of MNV. To study this, a mouse MNV model was established through laser injury, and the AIF-1 expression levels were then measured using western blot and immunohistochemistry. AIF-1 siRNA was intravitreally injected to silence AIF-1 gene expression. Western blot and choroidal flat mount were performed to measure the progression of MNV and proliferation of the CECs. These results showed that the protein expression of AIF-1 was significantly elevated in the laser-induced mouse MNV model, and the expression trend was consi...
Source: Experimental Eye Research - April 20, 2023 Category: Opthalmology Authors: Qinghua Li Liting Hu Guibo Liu Xiaoni Yin Ying Li Xiangyang Wei Ning Duan Xiaoran Zhao Qingyun Gong Zhaodong Du Source Type: research

RNA therapeutics in ophthalmology - translation to clinical trials.
Abstract The use of RNA interference technology has proven to inhibit the expression of many target genes involved in the underlying pathogenesis of several diseases affecting various systems. First established in in vitro and later in animal studies, small interfering RNA (siRNA) and antisense oligonucleotide (ASO) therapeutics are now entering clinical trials with the potential of clinical translation to patients. Gene-silencing therapies have demonstrated promising responses in ocular disorders, predominantly due to the structure of the eye being a closed and compartmentalised organ. However, although the effic...
Source: Experimental Eye Research - February 3, 2021 Category: Opthalmology Authors: Gupta A, Kafetzis KN, Tagalakis AD, Yu-Wai-Man C Tags: Exp Eye Res Source Type: research

Up-regulation of P-gp via NF- κB activation confers protection against oxidative damage in the retinal pigment epithelium cells.
In conclusion, P-gp can provide moderate cytoprotection for the human RPE cells by ameliorating the mitochondrial dysfunction and NF-κB activation may be a potential regulator of P-gp expression response to oxidative stress. PMID: 30496729 [PubMed - as supplied by publisher]
Source: Experimental Eye Research - November 26, 2018 Category: Opthalmology Authors: Feng Q, Yang W, Gao Z, Ruan X, Zhang Y Tags: Exp Eye Res Source Type: research

Nrf2 protects photoreceptor cells from photo-oxidative stress induced by blue light.
In this study, we evaluated the effect of blue light exposure on photoreceptors and its relationship to Nrf2 using cultured murine photoreceptor (661W) cells. 661W cells were exposed to blue light at 2500 lux. Exposure to blue light for 6-24 h resulted in a significant increase in intracellular reactive oxygen species (ROS) and death of 661W cells in a time-dependent fashion. Blue light exposure resulted in activation of Nrf2, as indicated by an increase in nuclear translocation of Nrf2. This was associated with a significant induction of expression of Nrf2 as well as an array of Nrf2 target genes, including antioxidant g...
Source: Experimental Eye Research - December 2, 2016 Category: Opthalmology Authors: Chen WJ, Wu C, Xu Z, Kuse Y, Hara H, Duh EJ Tags: Exp Eye Res Source Type: research