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Upregulated TTYH2 expression is critical for the invasion and migration of U2OS human osteosarcoma cell lines.
Abstract Ion channels have recently emerged as stable biomarkers and anticancer targets particularly when the applications of the currently available therapeutic regimens are limited, as in case of osteosarcoma, a malignant bone tumor. Here, we evaluated the expression of TTYH2, a presumably calcium-activated chloride channel, in a human osteosarcoma cell line U2OS. We used small-interfering RNA (siRNA)-mediated gene silencing to demonstrate the downregulation in the expression of TTYH2 that resulted in the decrease in the invasion and migration, but not proliferation, of U2OS cells. The expression levels of Slug ...
Source: Biochemical and Biophysical Research communications - June 19, 2019 Category: Biochemistry Authors: Moon DK, Bae YJ, Jeong GR, Cho CH, Hwang SC Tags: Biochem Biophys Res Commun Source Type: research

The short interference RNA (siRNA) targeting NMUR2 relieves nociception in a bone cancer pain model of rat through PKC-ERK and PI3K-AKT pathways.
CONCLUSION: si-NMUR2 alleviates BCP via inactivation of PKC/ERK and PI3K/AKT signal pathways. PMID: 30914203 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - March 22, 2019 Category: Biochemistry Authors: Peng S, Lu Y, Li P, Liu P, Shi X, Liu C, Zhang Y, Liu S, Wang J Tags: Biochem Biophys Res Commun Source Type: research

Long non-coding RNA THOR promotes human osteosarcoma cell growth in vitro and in vivo.
Abstract Long non-coding RNA (LncRNA) dysregulation is associated with human osteosarcoma (OS) cell progression. Recent studies have characterized a novel but ultra-conserved LncRNA THOR ("Lnc-THOR") as a cancer-specific LncRNA, mediating cell growth. In the current study, we show that Lnc-THOR is expressed in established and primary human OS cells. It is also detected in human OS tissues, but not in the surrounding normal bone tissues. siRNA-induced knockdown or CRSIPR/Cas9-mediated knockout Lnc-THOR significantly inhibited human OS cell survival and proliferation. Insulin-like growth factor 2 mRNA-binding protei...
Source: Biochemical and Biophysical Research communications - April 4, 2018 Category: Biochemistry Authors: Chen W, Chen M, Xu Y, Chen X, Zhou P, Zhao X, Pang F, Liang W Tags: Biochem Biophys Res Commun Source Type: research

Che-1 gene silencing by inhibiting mutant p53 expression.
In this study, we aimed to investigate the effects and specific mechanism of Che-1 in the regulation of osteosarcoma (OS) cell growth. We found that Che-1 is highly expressed in several kinds of OS cells compared with osteoblast hFOB1.19 cells. MTT and flow cytometry assays showed that Che-1 depletion by siRNA markedly suppressed MG-63 and U2OS cell proliferation and promoted apoptosis. The chromatin immunoprecipitation (ChIP) assay verified the presence of Che-1 on the p53 promoter in MG-63 and U2OS cells carrying mutant p53. Further studies showed that Che-1 depletion inhibited mutant p53 expression. Notably, our study s...
Source: Biochemical and Biophysical Research communications - March 20, 2016 Category: Biochemistry Authors: Liu M, Wang D, Li N Tags: Biochem Biophys Res Commun Source Type: research

C6 ceramide sensitizes pemetrexed-induced apoptosis and cytotoxicity in osteosarcoma cells.
Abstract Chemotherapy has significantly improved the prognosis of high-grade osteosarcoma (OS), but over 30% of OS patients can still not be cured. Pemetrexed, the newly-developed anti-folate chemotherapy drug, exerted lower efficacy against OS cells. Here, we aimed to increase pemetrexed efficiency, and found that the cell-permeable short-chain ceramide (C6) significantly enhanced pemetrexed-induced viability reduction and death in cultured OS cell lines (U2OS and MG-63). Pemetrexed induced moderate apoptosis in OS cells, which was dramatically augmented by C6 ceramide. The apoptosis inhibitor z-VAD-fmk largely i...
Source: Biochemical and Biophysical Research communications - August 21, 2014 Category: Biochemistry Authors: Zhu X, Du X, Deng X, Yi H, Cui S, Liu W, Shen A, Cui Z Tags: Biochem Biophys Res Commun Source Type: research

Acute dyskerin depletion triggers cellular senescence and renders osteosarcoma cells resistant to genotoxic stress-induced apoptosis.
Abstract Dyskerin is a conserved, nucleolar RNA-binding protein implicated in an increasing array of fundamental cellular processes. Germline mutation in the dyskerin gene (DKC1) is the cause of X-linked dyskeratosis congenita. Conversely, wild-type dyskerin is overexpressed in sporadic cancers, and high-levels may be associated with poor prognosis. It was previously reported that acute loss of dyskerin function via siRNA-mediated depletion slowed the proliferation of transformed cell lines. However, the mechanisms remained unclear. Using human U2OS osteosarcoma cells, we show that siRNA-mediated dyskerin depletio...
Source: Biochemical and Biophysical Research communications - March 29, 2014 Category: Biochemistry Authors: Lin P, Mobasher ME, Alawi F Tags: Biochem Biophys Res Commun Source Type: research