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Specialty: Genetics & Stem Cells
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Total 47651 results found since Jan 2013.
Rational design of eukaryotic riboswitches that upregulate IRES-mediated translation initiation with high switching efficiency through a kinetic trapping mechanism < em > in vitro < /em >
RNA. 2023 Sep 13:rna.079778.123. doi: 10.1261/rna.079778.123. Online ahead of print.ABSTRACTIn general, riboswitches functioning through a co-transcriptional kinetic trapping mechanism (kt-riboswitches) show higher switching efficiencies in response to practical concentrations of their ligand molecules than eq-riboswitches, which function by an equilibrium mechanism. However, the former have been much more difficult to design due to their more complex mechanism. We here successfully developed a rational strategy for constructing eukaryotic kt-riboswitches that ligand-dependently enhance translation initiation mediated by a...
Source: RNA - September 13, 2023 Category: Genetics & Stem Cells Authors: Hajime Takahashi Masahiro Fujikawa Atsushi Ogawa Source Type: research
Potential of mesenchymal stem cell-derived conditioned medium/secretome as a therapeutic option for ocular diseases
Regen Med. 2023 Sep 13. doi: 10.2217/rme-2023-0089. Online ahead of print.ABSTRACTResearch has shown that the therapeutic effect of mesenchymal stem cells (MSCs) is partially due to its secreted factors as opposed to the implantation of the cells into the treated tissue or tissue replacement. MSC secretome, especially in the form of conditioned medium (MSC-CM) is now being explored as an alternative to MSCs transplantation. Despite the observed benefits of MSC-CM, only a few clinical trials have evaluated it and other secretome components in the treatment of eye diseases. This review provides insight into the potential the...
Source: Regenerative Medicine - September 13, 2023 Category: Genetics & Stem Cells Authors: Wesley Harrisson Bouche Djatche Huimin Zhu Wenlei Ma Yue Li Ziang Li Hong Zhao Zhizhen Liu Hua Qiao Source Type: research
A new era of understanding in vivo metabolic flux in thermogenic adipocytes
Curr Opin Genet Dev. 2023 Sep 11;83:102112. doi: 10.1016/j.gde.2023.102112. Online ahead of print.ABSTRACTNonshivering thermogenesis by brown adipose tissue (BAT) is an adaptive mechanism for maintaining body temperature in cold environments. BAT is critical in rodents and human infants and has substantial influence on adult human metabolism. Stimulating BAT therapeutically is also being investigated as a strategy against metabolic diseases because of its ability to function as a catabolic sink. Thus, understanding how brown adipocytes and the related brite/beige adipocytes use nutrients to fuel their demanding metabolism ...
Source: Current Opinion in Genetics and Development - September 13, 2023 Category: Genetics & Stem Cells Authors: John A Haley Cholsoon Jang David A Guertin Source Type: research
Rational design of eukaryotic riboswitches that upregulate IRES-mediated translation initiation with high switching efficiency through a kinetic trapping mechanism < em > in vitro < /em >
RNA. 2023 Sep 13:rna.079778.123. doi: 10.1261/rna.079778.123. Online ahead of print.ABSTRACTIn general, riboswitches functioning through a co-transcriptional kinetic trapping mechanism (kt-riboswitches) show higher switching efficiencies in response to practical concentrations of their ligand molecules than eq-riboswitches, which function by an equilibrium mechanism. However, the former have been much more difficult to design due to their more complex mechanism. We here successfully developed a rational strategy for constructing eukaryotic kt-riboswitches that ligand-dependently enhance translation initiation mediated by a...
Source: RNA - September 13, 2023 Category: Genetics & Stem Cells Authors: Hajime Takahashi Masahiro Fujikawa Atsushi Ogawa Source Type: research
New perspectives on the causes and consequences of male meiotic drive
Curr Opin Genet Dev. 2023 Sep 11:102111. doi: 10.1016/j.gde.2023.102111. Online ahead of print.ABSTRACTGametogenesis is vulnerable to selfish genetic elements that bias their transmission to the next generation by cheating meiosis. These so-called meiotic drivers are widespread in plants, animals, and fungi and can impact genome evolution. Here, we summarize recent progress on the causes and consequences of meiotic drive in males, where selfish elements attack vulnerabilities in spermatogenesis. Advances in genomics provide new insights into the organization and dynamics of driving chromosomes in natural populations. Commo...
Source: Current Opinion in Genetics and Development - September 13, 2023 Category: Genetics & Stem Cells Authors: C écile Courret Xiaolu Wei Amanda M Larracuente Source Type: research
A new era of understanding in vivo metabolic flux in thermogenic adipocytes
Curr Opin Genet Dev. 2023 Sep 11;83:102112. doi: 10.1016/j.gde.2023.102112. Online ahead of print.ABSTRACTNonshivering thermogenesis by brown adipose tissue (BAT) is an adaptive mechanism for maintaining body temperature in cold environments. BAT is critical in rodents and human infants and has substantial influence on adult human metabolism. Stimulating BAT therapeutically is also being investigated as a strategy against metabolic diseases because of its ability to function as a catabolic sink. Thus, understanding how brown adipocytes and the related brite/beige adipocytes use nutrients to fuel their demanding metabolism ...
Source: Current Opinion in Genetics and Development - September 13, 2023 Category: Genetics & Stem Cells Authors: John A Haley Cholsoon Jang David A Guertin Source Type: research
Potential of mesenchymal stem cell-derived conditioned medium/secretome as a therapeutic option for ocular diseases
Regen Med. 2023 Sep 13. doi: 10.2217/rme-2023-0089. Online ahead of print.ABSTRACTResearch has shown that the therapeutic effect of mesenchymal stem cells (MSCs) is partially due to its secreted factors as opposed to the implantation of the cells into the treated tissue or tissue replacement. MSC secretome, especially in the form of conditioned medium (MSC-CM) is now being explored as an alternative to MSCs transplantation. Despite the observed benefits of MSC-CM, only a few clinical trials have evaluated it and other secretome components in the treatment of eye diseases. This review provides insight into the potential the...
Source: Regenerative Medicine - September 13, 2023 Category: Genetics & Stem Cells Authors: Wesley Harrisson Bouche Djatche Huimin Zhu Wenlei Ma Yue Li Ziang Li Hong Zhao Zhizhen Liu Hua Qiao Source Type: research
Rational design of eukaryotic riboswitches that upregulate IRES-mediated translation initiation with high switching efficiency through a kinetic trapping mechanism < em > in vitro < /em >
RNA. 2023 Sep 13:rna.079778.123. doi: 10.1261/rna.079778.123. Online ahead of print.ABSTRACTIn general, riboswitches functioning through a co-transcriptional kinetic trapping mechanism (kt-riboswitches) show higher switching efficiencies in response to practical concentrations of their ligand molecules than eq-riboswitches, which function by an equilibrium mechanism. However, the former have been much more difficult to design due to their more complex mechanism. We here successfully developed a rational strategy for constructing eukaryotic kt-riboswitches that ligand-dependently enhance translation initiation mediated by a...
Source: RNA - September 13, 2023 Category: Genetics & Stem Cells Authors: Hajime Takahashi Masahiro Fujikawa Atsushi Ogawa Source Type: research
CircRNA_0088196 Regulates Trophoblast Proliferation and Apoptosis in Preeclampsia Through the miR-379-5p/HSPA5 Axis
Biochem Genet. 2023 Sep 12. doi: 10.1007/s10528-023-10506-6. Online ahead of print.ABSTRACTExisting research has confirmed the dysregulation of circular RNA (circRNA) in a wide variety of human diseases. Thus, in this study, we explored the potential mechanism of circRNA_0088196 in preeclampsia (PE). We performed quantitative real-time PCR to examine circRNA_0088196 expression and verified the function of circRNA_0088196 in vitro using CCK-8, TUNEL, flow cytometry, and Western blotting analyses. Additionally, we studied the mechanism using dual-luciferase reporter gene experiments. The results of our research revealed the ...
Source: Biochemical Genetics - September 12, 2023 Category: Genetics & Stem Cells Authors: Zhihui Xiong Qingmin Wang Shuping Pei Huiling Zheng Wei Wang Source Type: research
Population genetic analysis and scans for adaptation and contemporary selection footprints provide genomic insight into < em > aus < /em > , < em > indica < /em > and < em > japonica < /em > rice cultivars diversification
J Genet. 2023;102:43.ABSTRACTFollowing domestication, rice cultivars have been spread worldwide to different climates and have experienced selection pressures to improve desirable traits. This has resulted in diverse cultivars that display variations in phenotypic traits, such as stress tolerance, grain size, and yield. To better understand the genomic composition arising from cultivar's development and local adaptation, high-density genotypes (containing 286,183 single-nucleotide polymorphisms after the quality control) of 1284 rice cultivars of aus, indica, and temperate and tropical japonica were scanned for diversifyin...
Source: Journal of Genetics - September 12, 2023 Category: Genetics & Stem Cells Authors: Seyed Milad Vahedi Moslem Momen Seyedeh Fatemeh Mousavi Mohammad Hossein Banabazi Mohammad Saeed Hasanvandi Madhav Bhatta Mahmoud Amiri Roudbar Siavash Salek Ardestani Source Type: research
< em > In silico < /em > genotyping of blood group alleles using WGS data: a comparative study of the Orang Asli in Peninsular Malaysia
This study reports four alleles (rs12075, rs7683365, rs586178 and rs2298720) of DUFFY, MNS, RH and KIDD blood group systems which were significantly distinct between indigenous Orang Asli and cosmopolitanMalaysians. Eighteen alleles that belong to 14 blood group systems were found statistically distinct in comparison to global population datasets. Although not much significant differences were observed in phenotypes of most blood group systems, major insights were observed when comparing Orang Asli with aboriginal Australians and cosmopolitanMalaysians.This study serves as the first of its kind to utilize genomic data to i...
Source: Journal of Genetics - September 12, 2023 Category: Genetics & Stem Cells Authors: Mercy Rophina Teh Lay Kek Sridhar Sivasubbu Vinod Scaria Mohd Zaki Salleh Source Type: research
Overexpression of microRNA-130a-3p suppresses glucose lipid levels and oxidative damage in diabetic retinopathy mice via modulating cell division cycle 42
J Genet. 2023;102:41.ABSTRACTMicroRNA (miR)-130a-3p has been unraveled to exert effects on diabetes. However, the research for probing its role in diabetic retinopathy (DR) is limited. Our study intends to unravel the regulatory effects of miR-130a-3p on DR development via cell division cycle 42 (CDC42). The DR mouse model was established and the serum sample of DR patients was collected. The levels of miR- 130a-3p and CDC42 in DR mice and patients were detected. The nucleic acids modified miR-130a-3p or CDC42 were injected into DR mice to examine the change of glucose lipid levels, visual acuity, oxidative response and th...
Source: Journal of Genetics - September 12, 2023 Category: Genetics & Stem Cells Authors: Hui Wang Xu Dong Jing Zhou Caoyu Sun Source Type: research
A de novo 2.3 kb structural variant in MITF explains a novel splashed white phenotype in a Thoroughbred family
Anim Genet. 2023 Sep 12. doi: 10.1111/age.13352. Online ahead of print.ABSTRACTSplashed white in horses is characterized by extensive white patterning on the legs, face and abdomen and may be accompanied by deafness. To date, seven variants in microphthalmia-associated transcription factor (MITF) and two variants in Paired Box 3 (PAX3) have been identified to explain this phenotype. A splashed white Thoroughbred stallion, whose sire and dam were not patterned, was hypothesized to have a de novo variant leading to his white coat pattern. A whole-genome sequencing candidate gene approach identified two single nucleotide vari...
Source: Animal Genetics - September 12, 2023 Category: Genetics & Stem Cells Authors: R R Bellone J Tanaka E Esdaile R B Sutton F Payette L Leduc B J Till A K Abdel-Ghaffar M Hammond K G Magdesian Source Type: research
Candidate signatures of positive selection for environmental adaptation in indigenous African cattle: A review
Anim Genet. 2023 Sep 11. doi: 10.1111/age.13353. Online ahead of print.ABSTRACTEnvironmental adaptation traits of indigenous African cattle are increasingly being investigated to respond to the need for sustainable livestock production in the context of unpredictable climatic changes. Several studies have highlighted genomic regions under positive selection probably associated with adaptation to environmental challenges (e.g. heat stress, trypanosomiasis, tick and tick-borne diseases). However, little attention has focused on pinpointing the candidate causative variant(s) controlling the traits. This review compiled inform...
Source: Animal Genetics - September 12, 2023 Category: Genetics & Stem Cells Authors: Sumaya Kambal Abdulfatai Tijjani Sabah A E Ibrahim Mohamed-Khair A Ahmed Joram M Mwacharo Olivier Hanotte Source Type: research
