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Kisspeptin decreases the adverse effects of human ovarian vitrification by regulating ROS-related apoptotic occurrences
In this study, the effects of kisspeptin on the improvement of the vitrification and thawing results of human ovarian tissues were investigated. In this work, 12 ovaries from patients who underwent hysterectomy were collected laparoscopically, and then 32 samples from each of their tissues were taken. Haematoxylin and eosin (H&E) staining was performed to check the normality of the ovarian tissue and, subsequently, the samples were allocated randomly into four groups, including: (1) fresh (control), (2) vitrification, (3) vitrified + 1 μM kisspeptin, and (4) vitrified + 10 μM kisspeptin groups. After vitrification, t...
Source: Zygote - September 1, 2023 Category: Reproduction Medicine Authors: Anahita Tavakoli Fereshteh Aliakbari Malek Soleimani Mehranjani Source Type: research

Varicocelectomy improves sperm parameters, sperm DNA integrity as well as the other critical semen features
In this study, the semen samples from patients with left-side varicocele of grade II-III before and after laparoscopic varicocelectomy were compared to healthy individuals and separated into three groups. The volume of semen, sperm concentration (106/ml), motility (%), viability (%) and normal morphology (%) were assessed. Total antioxidant capacity (TAC), catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA) together with other biochemical substances in seminal plasma as alpha-glucosidase (α-Glu), fructose (Fr) and citric acid (CA) were determined by ELISA method. The spermatozoa activity including ion-tra...
Source: J Physiol Pharmacol - April 23, 2023 Category: Drugs & Pharmacology Authors: Y Nakonechnyi A Nakonechnyi M Fraczek A Havrylyuk M Kamieniczna V Chopyak M Kurpisz Source Type: research

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