• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

SRV2 promotes mitochondrial fission and Mst1-Drp1 signaling in LPS-induced septic cardiomyopathy.
In this study, we examined the mechanisms of SRV2-mediated mitochondrial fission in septic cardiomyopathy. Western blotting, ELISA, and immunofluorescence were used to evaluate mitochondrial function, oxidative balance, energy metabolism and caspase-related death, and siRNA and adenoviruses were used to perform loss- and gain-of-function assays. Our results demonstrated that increased SRV2 expression promotes, while SRV2 knockdown attenuates, cardiomyocyte death in LPS-induced septic cardiomyopathy. Mechanistically, SRV2 activation promoted mitochondrial fission and physiological abnormalities by upregulating oxidative inj...
Source: Aging - January 16, 2020 Category: Biomedical Science Authors: Shang X, Zhang Y, Xu J, Li M, Wang X, Yu R Tags: Aging (Albany NY) Source Type: research

Inhibited Corneal Neovascularization in Rabbits following Corneal Alkali Burn by Double-Target Interference for VEGF and HIF-1 α.
This study aims to investigate the synergistic effects of VEGF and HIF-1α gene silencing on alkali burn-induced CNV in rabbits. The models of rabbits in corneal alkali burn were established. SiRNA recombinant adenovirus was used to explore the synergistic effects of VEGF and HIF-1α gene silencing on alkali burn-induced CNV. CNV area and ultrastructure of cornea were observed. The expression of VEGF and HIF-1α was detected. CNV was observed in rabbits following alkali burn. In addition, overexpressed VEGF and HIF-1α was also observed in rabbits following alkali burn. Then, silencing HIF-1α or silencing VEGF decreased a...
Source: Bioscience Reports - October 24, 2018 Category: Biomedical Science Authors: Fu YC, Xin ZM Tags: Biosci Rep Source Type: research

Heme oxygenase-1 ameliorates oxidative stress-induced endothelial senescence via regulating endothelial nitric oxide synthase activation and coupling.
CONCLUSIONS: HO-1 ameliorated endothelial senescence through enhancing eNOS activation and defending eNOS uncoupling, suggesting that HO-1 is a potential target for treating endothelial senescence. PMID: 30048241 [PubMed - as supplied by publisher]
Source: Aging - July 24, 2018 Category: Biomedical Science Authors: Luo W, Wang Y, Yang H, Dai C, Hong H, Li J, Liu Z, Guo Z, Chen X, He P, Li Z, Li F, Jiang J, Liu P, Li Z Tags: Aging (Albany NY) Source Type: research

The mechanism of all-trans retinoic acid in the regulation of apelin expression in vascular endothelial cells.
Abstract The apelin gene can promote vascular endothelial cell (VEC) proliferation, migration and angiogenesis. However, the molecular mechanism for regulation of the apelin gene is still unknown. Real-time PCR and Western blotting analysis were employed to detect the effect of all-trans retinoic acid (ATRA) in up-regulating apelin expression in human umbilical vein endothelial cells (HUVECs). Furthermore, the in vivo study also indicated that ATRA could increase apelin expression in balloon-injured arteries of rats, which is consistent with the results from the cultured HUVECs. To ensure whether retinoic acid rec...
Source: Bioscience Reports - October 25, 2017 Category: Biomedical Science Authors: Shi H, Yuan L, Yang H, Zang A Tags: Biosci Rep Source Type: research

Sigmar1 regulates endoplasmic reticulum stress-induced C/EBP-homologous protein (CHOP) expression in cardiomyocytes.
Abstract C/EBP-homologous protein (CHOP) is a ubiquitously expressed stress-inducible transcription factor robustly induced by maladaptive endoplasmic reticulum (ER) stresses in a wide variety of cells. Here we examined a novel function of Sigma 1 receptor (Sigmar1) in regulating CHOP expression under ER-stress in cardiomyocytes. We also defined Sigmar1-dependent activation of the adaptive ER-stress pathway in regulating CHOP expression. We used adenovirus-mediated Sigmar1 overexpression as well as Sigmar1 knockdown by small interfering RNA (siRNA) in neonatal rat ventricular cardiomyocytes; to induce ER-stress ca...
Source: Bioscience Reports - June 30, 2017 Category: Biomedical Science Authors: Alam S, Abdullah CS, Aishwarya R, Orr AW, Traylor J, Miriyala S, Panchatcharam M, Pattillo CB, Bhuiyan MS Tags: Biosci Rep Source Type: research

PGC-1α Limits AngiotensinII-Induced Rat Vascular Smooth Muscle Cells Proliferation via Attenuating NOX1-Mediated Generation of Reactive Oxygen Species.
Abstract Angiotensin II (AngII)-induced excessive reactive oxygen species (ROS) generation and proliferation of vascular smooth muscle cells (VSMCs) is a critical contributor to the pathogenesis of atherosclerosis. Peroxisome proliferator-activated receptor γ (PPARγ) coactivator-1α (PGC-1α) is involved in the regulation of ROS generation, VSMCs proliferation and energy metabolism. The aim of this study was to investigate whether PGC-1α mediates AngII-induced ROS generation and VSMCs hyperplasia. Our results showed that the protein content of PGC-1α was negatively correlated with an increase in cell prolifera...
Source: Bioscience Reports - August 26, 2015 Category: Biomedical Science Authors: Zhao Q, Zhang J, Wang H Tags: Biosci Rep Source Type: research

Role of the Prostaglandin E2/E-Prostanoid 2 Receptor Signaling Pathway in TGF-β-induced Mice Mesangial Cell damage.
Abstract The prostaglandin E2 receptor, E-prostanoid 2 (EP2), plays important roles in mice glomerular mesangial cell (MCs) damage induced by transforming growth factor-β1(TGF β1), however the molecular mechanisms for this remain unknown. The present study examined the role of the EP2 signaling pathway in TGF-β1-induced MCs proliferation, extracellular matrix (ECM) accumulation and expression of prostaglandin E2 synthase (PGES). We generated primary mice MCs. Results showed MCs proliferation promoted by TGF-β1 were increased, however, the production of Cyclic Adenosine 3',5'-Monophosphate (cAMP) and prostaglan...
Source: Bioscience Reports - October 20, 2014 Category: Biomedical Science Authors: Li NN, Xu YY, Chen XL, Fan YP, Wu JH Tags: Biosci Rep Source Type: research