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Characterization of In vitro inhibitory effects of consensus short interference RNAs against non-structural 5B gene of hepatitis C virus 1a genotype
Conclusions: Stable Huh-7 cells persistently expressing NS5B protein should be helpful for molecular pathogenesis of HCV infection and development of anti-HCV drug screening assays. The siRNA was effective against NS5B and could be considered as an adjuvant therapy along with other promising anti-HCV regimens.
Source: Indian Journal of Medical Microbiology - March 17, 2019 Category: Microbiology Authors: Imran Shahid Waleed Hassan Almalki Munjed M Ibrahim Sultan Ahmad Alghamdi Mohammed H Mukhtar Shaia Saleh R. Almalki Saad Ahmed Alkahtani Mohammad S Alhaidari Source Type: research

Transcription Factor MafB Suppresses Type I Interferon Production by CD14+ Monocytes in Patients With Chronic Hepatitis C
Transcription factor MafB regulates differentiation and activity of monocytes/macrophage, and is associated with the development of atherosclerosis and cancers. However, the role of MafB in modulation of CD14+ monocytes in chronic viral hepatitis was not fully elucidated. Thus, the aim of current study was to investigate the immunoregulatory function of MafB to type I interferon (IFN) secretion by CD14+ monocytes and its contribution to pathogenesis of chronic hepatitis C virus (HCV) infection. A total of twenty-nine chronic hepatitis C patients and twenty-one healthy individuals were enrolled. Serum IFN-α1 and IFN-β was...
Source: Frontiers in Microbiology - August 6, 2019 Category: Microbiology Source Type: research

Enhancement of the replication of HCV replicons of genotypes 1-4 by manipulation of CpG and UpA dinucleotide frequencies and use of cell lines expressing SECL14L2 - application for antiviral resistance testing.
Abstract Treatment for hepatitis C virus (HCV) has improved greatly through the use of direct acting antivirals (DAAs). However, their effectiveness and potential for drug resistance development in non-genotype 1 variants of HCV remains relatively unexplored as in vitro assays to assess drug susceptibility are poorly developed and unsuited for a transient transfection format. In the current study, we have evaluated effects of dinucleotide frequency changes in the replicon and the use of a SEC14L2-expressing cell line on the replication of HCV of different genotypes and evaluated the resulting assay formats for sus...
Source: Antimicrobial Agents and Chemotherapy - March 7, 2016 Category: Microbiology Authors: Witteveldt J, Martin-Gans M, Simmonds P Tags: Antimicrob Agents Chemother Source Type: research

USP18 Mediates Interferon Resistance of Dengue Virus Infection
Previous studies demonstrated that dengue virus (DENV) infection developed resistance to type-I interferons (IFNα/β). The underlying mechanism remains unclear. USP18 is a negative regulator of IFNα/β signaling, and its expression level is significantly increased following DENV infection in cell lines and patients’ blood. Our previous study revealed that increased USP18 expression contributed to the IFN-α resistance of Hepatitis C Virus (HCV). However, the role of USP18 in DENV replication and resistance to IFN-α is elusive. In this current study, we aimed to explore the role of USP18 in DENV-2 replication and resis...
Source: Frontiers in Microbiology - April 30, 2021 Category: Microbiology Source Type: research