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Specialty: Biology
Source: Life Sciences
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Total 146 results found since Jan 2013.
The activation of the G protein-coupled estrogen receptor (GPER) prevents and regresses cardiac hypertrophy
Publication date: Available online 28 December 2019Source: Life SciencesAuthor(s): Romina A. Di Mattia, Juan I.E. Mariángelo, Paula G. Blanco, Carolina Jaquenod De Giusti, Enrique L. Portiansky, Cecilia B. Mundiña-Weilenmann, Ernesto A. Aiello, Alejandro OrlowskiAbstractVentricular hypertrophy is a risk factors for arrhythmias, ischemia and sudden death. It involves cellular modifications leading to a pathological remodeling and is associated with heart failure. The activation of the G protein-coupled estrogen receptor (GPER) mediates beneficial actions in the cardiovascular system. Our goal was to prevent and regress th...
Source: Life Sciences - December 30, 2019 Category: Biology Source Type: research
Dihydroartemisinin inhibits endothelial cell tube formation by suppression of the STAT3 signaling pathway
Publication date: Available online 24 December 2019Source: Life SciencesAuthor(s): Peng Gao, Li-li Wang, Jing Liu, Fengyun Dong, Wei Song, Lin Liao, Bei Wang, Wenqian Zhang, Xia Zhou, Qi Xie, Rong Sun, Ju LiuAbstractAimsEndothelial cell (EC) tube formation is crucial for tumor angiogenesis, which becomes a target for chemotherapy. The anti-malaria agent dihydroartemisinin (DHA) inhibited tumor growth and angiogenesis. The aim of this study was to investigate the effects of DHA on EC tube formation and the underlying mechanisms.Materials and methodsHuman umbilical vein endothelial cells (HUVECs) were cultured with different...
Source: Life Sciences - December 26, 2019 Category: Biology Source Type: research
BCLAF1 promotes cell proliferation, invasion and drug-resistance though targeting lncRNA NEAT1 in hepatocellular carcinoma
Publication date: Available online 20 December 2019Source: Life SciencesAuthor(s): Shao-Jiao Mou, Peng-Fei Yang, Yi-Pin Liu, Ning Xu, Wei-Wei Jiang, Wen-Jing YueAbstractAimsIn the present research, we aimed to investigate the effect of Bcl-2-associated transcription factor 1 (BCLAF1) on hepatocellular carcinoma and further explore the special molecular mechanism.Main methodsThe expression of BCLAF1 was analyzed in tumor tissues and different hepatocellular cancer cell lines by real-time RT-PCR and Western blot. Cell proliferation and invasion was explored using MTT and Transwell assay respectively. In addition, luciferase ...
Source: Life Sciences - December 20, 2019 Category: Biology Source Type: research
TEX19 promotes ovarian carcinoma progression and is a potential target for epitope vaccine immunotherapy
This study aimed to reveal the role of TEX19 in ovarian cancer (OC) and searched for potential candidate epitope peptides of TEX19 to facilitate clinical application.Main methodsTEX19 levels were evaluated by immunohistochemistry (IHC) in 98 human ovarian tissue samples. The correlation of TEX19 levels with patients' clinicopathological features was assessed. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting analysis were utilized to detect TEX19 levels in ovarian cell lines and TEX19-deficient cells. The level of TEX19 in OVCAR-3 and A2780 was knocked down by small interfering RNA (siRNA), an...
Source: Life Sciences - December 14, 2019 Category: Biology Source Type: research
NALP3 orchestrates cellular bioenergetics to facilitate non-small cell lung cancer cell growth
This study was to unravel the mechanism of NALP3 on modulating NSCLC cancer cell growth.MethodsIHC and immuno-blot were performed to analyze expression of NALP3 and indicated molecules. CCK-8 and xenograft nude mice assay were used to evaluate cell growth in vitro and in vivo. Bioenergetics assay was performed to measure OXPHOS and aerobic glycolysis. siRNA and shRNA were constructed to knockdown endogenous NALP3 and DNMT1. Co-immunoprecipitation was applied to confirm the interaction between NALP3 and DMAP1. BioProfile FLEX analyzer and Lactate Reagent Kit were used to measure relative level glucose uptake and lactate pro...
Source: Life Sciences - December 14, 2019 Category: Biology Source Type: research
HMGB1 upregulates NF-kB by inhibiting IKB-α and associates with diabetic retinopathy
This study aimed to investigate the effect of high-mobility group box 1 (HMGB1) protein and its mechanism in diabetic retinopathy (DR) were investigated.Main methodsHuman retinal endothelial cells (HREC) were uesd for chip-seq. Sprague Dawley (SD) rats were randomly divided into control group, HMGB1 group, diabetes mellitus (DM) combined with HMGB1 siRNA group, and DM group. Next, eyeballs were removed and retinas were detached for western blot. The DM model of cell was built by increasing the glucose concentration in cell culture medium. The regulation of HMGB1 was achieved by short hairpin (sh)-HMGB1 transfection, then, ...
Source: Life Sciences - December 7, 2019 Category: Biology Source Type: research
Amentoflavone suppresses amyloid β1–42 neurotoxicity in Alzheimer's disease through the inhibition of pyroptosis
ConclusionAF inhibits Aβ1-42-induced neurotoxicity in animal and cellular models through AMPK/GSK3β-mediated pyroptosis suppression. Our results highlight AF as a clinical compound for the prevention and treatment of AD.
Source: Life Sciences - November 16, 2019 Category: Biology Source Type: research
Amentoflavone suppresses Amyloid β1–42 neurotoxicity in Alzheimer’s disease through the inhibition of pyroptosis
ConclusionAF inhibits Aβ1-42-induced neurotoxicity in animal and cellular models through AMPK/GSK3β-mediated pyroptosis suppression. Our results highlight AF as a clinical compound for the prevention and treatment of AD.
Source: Life Sciences - November 10, 2019 Category: Biology Source Type: research
NLRP3 inflammasome mediate palmitate-induced endothelial dysfunction
This study aimed to investigate the role of NLRP3 (NOD-like receptor pyrin domain containing-3) inflammasome in FFA induced endothelial dysfunction.Main methodsHUVECs were transfected with NLRP3 siRNA and then stimulated with LPS and palmitate. C57 BL/6J mice transfected with NLRP3 Lenti-Virus were fed with a high-fat diet (HFD). The levels of NLRP3 inflammasome, AMPKα (AMP-activated protein kinase), endothelial nitric oxide synthase (eNOS) and the activity of the insulin signal pathway, in endothelial cells were determined via Western blotting. Endothelial function was determined by measuring the level of endothelium-dep...
Source: Life Sciences - November 7, 2019 Category: Biology Source Type: research
TBX3 deficiency accelerates apoptosis in cardiomyoblasts through regulation of P21 expression
In conclusion, we demonstrated that TBX3 deficiency accelerated apoptosis via directly regulating P21 expression in senescent cardiomyoblasts.
Source: Life Sciences - November 6, 2019 Category: Biology Source Type: research
Elevated levels of 15-lipoxygenase-1 contribute to the abnormal phenotypes of osteoblasts in human osteoarthritis
In conclusion, our results suggested that the expression of 15-LOX-1 on osteoblasts from the subchondral bone increased in OA. 15-LOX-1 inhibited autophagy by activated mTORC1, which in turn upregulated the markers of abnormal osteoblast phenotypes RUNX2, COL1, and OCN.
Source: Life Sciences - November 6, 2019 Category: Biology Source Type: research
HMGB1/TLR4 promotes apoptosis and reduces autophagy of hippocampal neurons in diabetes combined with OSA
In conclusion, IH aggravated apoptosis and autophagy defects in T2DM mice, and increased the protein expression of HMGB1 and TLR4. This was also confirmed in HG +IH-treated hippocampal HT22 cells. HMGB1 siRNA can significantly reduce the protein expression of HMGB1 and TLR4, reverse neuronal apoptosis and enhance autophagy.SignificanceWe believe that HMGB1 is a key factor in the regulation of hippocampal neuronal apoptosis and autophagy defects in T2DM combined with OSA. Targeting HMGB1/TLR4 signaling as a novel approach may delay or prevent the increased apoptosis and decreased autophagy induced by T2DM combined with OSA,...
Source: Life Sciences - November 1, 2019 Category: Biology Source Type: research
Abnormal thyroid hormone receptor signaling in osteoarthritic osteoblasts regulates microangiogenesis in subchondral bone
Publication date: Available online 22 October 2019Source: Life SciencesAuthor(s): Li Lei, Meng Li, Yiqun Pang, Jun Wang, Yunpeng Wan, Chen Zhu, Yin ZongshengAbstractAimsPrevious study indicated that the increase of local bio-availability of 3′3′5-triiodothyronine (T3) influenced osteoarthritis (OA) initiation. We aimed to investigate the role of thyroid hormone receptors (THRs) signaling in OA osteoblasts.Materials and methodsTHRs expression in OA was detected by immunohistochemistry, immunofluorescence, RT-qPCR and western blotting. These effects on the expression of angiogenesis-related factors were examined after TH...
Source: Life Sciences - October 23, 2019 Category: Biology Source Type: research
LncRNA Snhg3 contributes to dysfunction of cerebral microvascular cells in intracerebral hemorrhage rats by activating the TWEAK/Fn14/STAT3 pathway
In conclusion, the lncRNA Snhg3 contributes to dysfunction of cerebral microvascular cells in ICH rats by activating the TWEAK/Fn14/STAT3 pathway.
Source: Life Sciences - October 11, 2019 Category: Biology Source Type: research
Acetyl-11-keto-β-boswellic acid regulates the repair of rat sciatic nerve injury by promoting the proliferation of Schwann cells
This study aimed to study the effects of acetyl-11-keto-β-boswellic acid (AKBA) on the regeneration of injured peripheral nerves and the ability of the extracellular signal-regulated kinase（ERK） signaling pathway to regulate the proliferation of Schwann cells and the formation of myelin.Main methodsA sciatic nerve crush injury model rats were randomly divided into the model control, low-, medium-, and high-dose AKBA groups. The repair of myelin damage was observed through Luxol Fast Blue staining and the expression of neurofilament-200 (NF200) protein was detected through immunohistochemical tests. The relative expres...
Source: Life Sciences - October 11, 2019 Category: Biology Source Type: research
