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Source: Life Sciences
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Total 146 results found since Jan 2013.
TLR4 siRNA inhibits proliferation and invasion in colorectal cancer cells by downregulating ACAT1 expression
Publication date: Available online 10 May 2016 Source:Life Sciences Author(s): Kai Ye, Yiyang Wu, Yafeng Sun, Jian'an Lin, Jianhua Xu Aims Toll-like receptor 4 (TLR4) is involved in tumor development. Numerous studies have confirmed that TLR4 mediates processes in tumorigenesis, for example, inflammation, proliferation and invasion. However, the effects of TLR4 on colorectal cancer development have not been fully elucidated. The present study aimed to evaluate the effects and mechanisms of TLR4 on colorectal cancer development. Main methods The expression of TLR4 and Acyl coenzyme A cholesterol acyltransferase 1 (A...
Source: Life Sciences - May 10, 2016 Category: Biology Source Type: research
Downregulation of endothelin A receptor (ETaR) ameliorates renal ischemia reperfusion injury by increasing nitric oxide production
Publication date: Available online 7 May 2019Source: Life SciencesAuthor(s): Long Li, Xia Wang, Long Zheng, Jiawei Li, Ming Xu, Ruiming Rong, Tongyu Zhu, Yichen JiaAbstractAimsTo investigate the protective effects of downregulating ETaR expression on renal ischemia reperfusion injury (IRI).Main methodsThe renal IRI model was generated by clamping the left renal artery for 60 min followed by nephrectomy of the right kidney. ETaR siRNA were perfused through the renal artery during ischemia. HE staining was performed to assess histological injury. PCR was performed to determine the expression of NF-κb, TNF-α, IFN-γ, IL-6...
Source: Life Sciences - May 8, 2019 Category: Biology Source Type: research
Effects of MFHAS1 on cognitive impairment and dendritic pathology in the hippocampus of septic rats
Publication date: Available online 30 August 2019Source: Life SciencesAuthor(s): Jing Zhong, Chenyue Guo, Wenting Hou, Na Shen, Changhong MiaoAbstractAimsTo investigate the effects of malignant fibrous histiocytoma amplified sequence 1 (MFHAS1) on cognitive dysfunction, the expression of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and amyloid β peptide (Aβ) in the hippocampus, as well as dendritic pathology in the hippocampal CA1 region in sepsis-associated encephalopathy (SAE) rats.Main methodsThe rats were randomly divided into four groups: 1) control group (subjected to sham surgery), 2) control plus Mfhas...
Source: Life Sciences - September 1, 2019 Category: Biology Source Type: research
Depletion of CPEB1 protects against oxidized LDL-induced endothelial apoptosis and inflammation though SIRT1/LOX-1 signalling pathway
Publication date: Available online 12 September 2019Source: Life SciencesAuthor(s): Kaicheng Xu, Xiwen liu, Guanghao Ren, Dexin Yin, Suli Guo, Yue ZhaoAbstractAtherosclerosis (AS) is a chronic inflammatory disease that results from Oxidized low-density lipoprotein (Ox-LDL) induced endothelial dysfunction. Cytoplasmic polyadenylation element binding protein 1 (CPEB1) is closely related to the development of epithelial cells, but the role of CPEB1 in AS remains unknown. The RNA and protein levels of CPEB1 expression are increased by Ox-LDL exposure, which is abrogated by c-Jun amino-terminal kinase (JNK) inhibitor SP600125. ...
Source: Life Sciences - September 13, 2019 Category: Biology Source Type: research
Kaempferol targets Krt-14 and induces cytoskeletal mineralization in osteoblasts: A mechanistic approach
Publication date: Available online 5 March 2016 Source:Life Sciences Author(s): Vikram Khedgikar, Priyanka Kushwaha, Jyoti Gautam, Shewta Sharma, Ashwni Verma, Dharmendra Choudhary, Prabhat R. Mishra, Ritu Trivedi Kaempferol (KEM) has been observed to stimulate Krt-14 protein which subsequently contributes to matrix maturation and mineralization in rat primary osteoblast cells. Incorporation of Krt-14 siRNA results in reduced mRNA and protein expression after 48h post transfection and remained low for 9days. At day 9 Krt-14 siRNA significantly reduced mineralization without concomitant change in the cell number. ...
Source: Life Sciences - March 6, 2016 Category: Biology Source Type: research
Small interfering RNA targeting alpha7 nicotinic acetylcholine receptor sensitizes hepatocellular carcinoma cells to sorafenib
Publication date: Available online 18 January 2020Source: Life SciencesAuthor(s): Khalil Hajiasgharzadeh, Mohammad Hossein Somi, Behzad Mansoori, Vahid Khaze Shahgoli, Afshin Derakhshani, Ahad Mokhtarzadeh, Dariush Shanehbandi, Behzad BaradaranAbstractAimsIt has been demonstrated that reduced expression of alpha7 nicotinic acetylcholine receptor (α7nAChR) led to reduced chemotherapeutic drugs resistance in various cancer cells. However, whether small interfering RNA (siRNA) mediated knockdown of α7nAChR can reduce sorafenib (SOR) resistance in HCC cells remains to be determined.Materials and methodsThe effects of α7nACh...
Source: Life Sciences - January 18, 2020 Category: Biology Source Type: research
Mucin 1 downregulation impairs the anti-necroptotic effects of glucocorticoids in human bronchial epithelial cells
Publication date: Available online 7 February 2019Source: Life SciencesAuthor(s): Huojun Zhang, Qian Liu, Luxia Kong, Shuyun XuAbstractAimsTo investigate whether mucin 1 (MUC1) downregulation reduced the sensitivity of tumor necrosis factor-alpha (TNF-α)-induced bronchial epithelial cells to glucocorticoid-mediated necroptosis and explore the underlying mechanisms.Main methodsThe human lung bronchial epithelial cell line (16HBE) was transfected with small interfering RNA (siRNA) against MUC1 and then stimulated by TNF-α, where some cells were pretreated with dexamethasone. Flow cytometry was performed to analyze necropto...
Source: Life Sciences - February 7, 2019 Category: Biology Source Type: research
Synergistic effects of 7-O-geranylquercetin and siRNAs on the treatment of human breast cancer
Publication date: Available online 19 April 2019Source: Life SciencesAuthor(s): Jiaxin Zuo, Yameng Jiang, Enxia Zhang, Yuling Chen, Ze Liang, Jie Zhu, Yinan Zhao, Hong Xu, Guoliang Liu, Jiasi Liu, Wei Wang, Shubiao Zhang, Yuhong ZhenAbstractAimsTo investigate the antitumor effect of 7-O-geranylquercetin (GQ) combining with survivin siRNA (siSuvi) or IL-10 siRNA (siIL-10) to breast cancer.Main methodsXenograft tumor model was established by subcutaneously inoculating human breast cancer MCF-7 cells in BALB/c nude mice. Transfection efficiency of siRNA mediated by cationic liposome CDO14 in MCF-7 cells and tumor bearing mice...
Source: Life Sciences - April 19, 2019 Category: Biology Source Type: research
Role of eukaryotic translation initiation factors 3a in hypoxia-induced right ventricular remodeling of rats
Publication date: Available online 2 December 2015 Source:Life Sciences Author(s): Wen-Qun Li, Xiao-Hui Li, Yue-Han Wu, Jie Du, Ai-Ping Wang, Dai Li, Yuan-Jian Li Aim Eukaryotic translation initiation factors 3a (eIF3a) is involved in regulating cell cycle, cell division, growth and differentiation. Previous studies suggest a role of eIF3a on fibrosis disease and cellular proliferation and differentiation of fibroblasts. The present study aims to investigate the role of eIF3a on hypoxia-induced right ventricular (RV) remodeling and underlying mechanism. Main methods RV remodeling was induced by hypoxia (10% O2, 3...
Source: Life Sciences - December 3, 2015 Category: Biology Source Type: research
SIRT1 regulates lipopolysaccharide-induced CD40 expression in renal medullary collecting duct cells by suppressing the TLR4-NF- κB signaling pathway
Publication date: Available online 1 December 2016 Source:Life Sciences Author(s): Qin-qin Lin, Yuan-wen Geng, Zhong-wei Jiang, Zhen-jun Tian Aims Recent evidence indicates that sirtuin1 (SIRT1), a NAD+-dependent deacetylase, exerts a protective effect against inflammatory kidney injury by suppressing pro-inflammatory cytokines production. The co-stimulatory molecule, CD40, is expressed in a variety of inflammatory diseases in the kidney. Here, we aimed to investigate the potential effect of SIRT1 on CD40 expression induced by lipopolysaccharide (LPS) and to disclose the underlying mechanisms in renal inner medullary coll...
Source: Life Sciences - November 30, 2016 Category: Biology Source Type: research
Osteopontin plays a unique role in resistance of CD34+/CD123+ human leukemia cell lines KG1a to parthenolide
Conclusion Parthenolide in spite of the reduction in gene expression of AKT, mTOR or beta-catenin, stimulates the OPN expression in KG1a cells. The OPN expression pattern in KG1a cells could be compatible with CD34+/CD123+ subtype enrichment by PTL which in turn implies OPN's unique role in resistance of cell populations characterized by CD34+/CD123+ phenotype.
Source: Life Sciences - September 23, 2017 Category: Biology Source Type: research
TNF- α increases Staphylococcus aureus-induced death of human alveolar epithelial cell line A549 associated with RIP3-mediated necroptosis
Publication date: 15 February 2018 Source:Life Sciences, Volume 195 Author(s): Shun-hang Wen, Luo-na Lin, Hu-jun Wu, Lu Yu, Li Lin, Li-li Zhu, Hai-yan Li, Hai-lin Zhang, Chang-chong Li Aim To explore the role of tumor necrosis factor-alpha (TNF-α) on Staphylococcus aureus-induced necroptosis in alveolar epithelial cells. Main methods The A549 alveolar epithelial cell line was pretreated with small interfering RNA (siRNA) against receptor interacting protein-3 (RIP3) and then stimulated by S. aureus, where some cells were pretreated with TNF-α or TNF-α with anti-TNF-α antibody simultaneously. A549 cell death was assess...
Source: Life Sciences - January 12, 2018 Category: Biology Source Type: research
Regulation of apolipoprotein A-I gene expression by the histamine H1 receptor: Requirement for NF-κB
Publication date: 1 September 2018Source: Life Sciences, Volume 208Author(s): Michael J. Haas, Marilu Jurado-Flores, Ramadan Hammoud, Gabriela Plazarte, Luisa Onstead-Haas, Norman C.W. Wong, Arshag D. MooradianAbstractAimsEarlier it had been found by us that apolipoprotein A-I (apo A-I) is suppressed by histamine in HepG2 cells. Histamine has been shown to regulate NF-κB activity, though not in hepatocytes. Therefore we examined the role of the histamine receptors and NF-κB in histamine-mediated apo A-I gene expression in HepG2 liver cells.Main methodsThe effect of histamine on histamine H1 receptor expression, and NF-κ...
Source: Life Sciences - July 19, 2018 Category: Biology Source Type: research
ERK and miRNA-1 target Cx43 expression and phosphorylation to modulate the vascular protective effect of angiotensin II
In this study, we investigated whether the beneficial effects of AngII are related to regulation of gap junctions (GJs) and connexin43 (Cx43), and the implication of MAPK signaling and microRNA (miR-1) in this process.Key findingsOur results show that after hemorrhagic shock or hypoxia, the blockade of GJs or knockdown of Cx43 inhibits the AngII-induced increase in vascular reactivity of superior mesenteric arteries and the contractile response of vascular smooth muscle cells (VSMCs). AngII treatment increases Cx43 expression and phosphorylation at Ser262, and restores gap-junctional communication (GJIC) between VSMCs afte...
Source: Life Sciences - November 9, 2018 Category: Biology Source Type: research
LncRNA Snhg3 contributes to dysfunction of cerebral microvascular cells in intracerebral hemorrhage rats by activating the TWEAK/Fn14/STAT3 pathway
In conclusion, the lncRNA Snhg3 contributes to dysfunction of cerebral microvascular cells in ICH rats by activating the TWEAK/Fn14/STAT3 pathway.
Source: Life Sciences - October 11, 2019 Category: Biology Source Type: research
