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EGb 761 protects cardiac microvascular endothelial cells against hypoxia/reoxygenation injury and exerts inhibitory effect on ATM pathway.
In this study, H/R-injuried MVECs were treated with EGb 761, then cell viability, apoptosis, ROS production, SOD activity, caspase-3 activity, and the protein level of ATM, γ-H2AX, p53, Bax were measured. ATM siRNA was transfected to study the changes of protein in ATM pathway. EGb 761 presented protective effect on H/R-injuried MVECs with decreasing cell death, apoptosis and ROS, and elevated SOD activity. Next, EGb 761 could inhibit H/R-induced ATM, γ-H2AX, p53, Bax in a dose-dependent manner. Moreover, ATM siRNA also could inhibit H/R-induced ATM, γ-H2AX, p53, Bax. Overall, these findings verify EGb 761 protects card...
Source: Journal of Microbiology and Biotechnology - December 13, 2016 Category: Biotechnology Authors: Zhang C, Wang DF, Zhang Z, Han D, Yang K Tags: J Microbiol Biotechnol Source Type: research

Histone H3 is digested by Granzyme A during compromised cell death in the Raji cells.
Abstract Granzyme A (GzmA) was identified as a cytotoxic T lymphocyte protease protein expressed at nucleus. A number of nuclear proteins are well known as GzmA substrates, and GzmA is related with caspase independent apoptosis. Histones H1, H2B, and H3 were identified as GzmA substrates through in vitro experiment with purified nucleosome. Here, we demonstrated that histone H3 was cleaved by GzmA in vivo during staurosporine-induced cell death. Moreover, histone H3 cleavage was blocked by the GzmA inhibitor nafamostat mesylate and by GzmA knockdown using siRNA. Taken together we verified that histone H3 is a real...
Source: Journal of Microbiology and Biotechnology - June 2, 2015 Category: Biotechnology Authors: Lee PY, Park BC, Chi SW, Bae KH, Kim S, Cho S, Kim JH, Park SG Tags: J Microbiol Biotechnol Source Type: research

Tumor suppressor protein p53 promotes 2-methoxyestradiol-induced activation of Bak and Bax, leading to mitochondria-dependent apoptosis in human colon cancer HCT116 cells.
Abstract To examine the effect of tumor suppressor protein p53 on the antitumor activity of 2-methoxyestradiol (2-MeO-E2), 2-MeO-E2-induced cell cycle changes and apoptotic events were compared between human colon carcinoma cell lines HCT116 (p53+/+) and HCT116 (p53-/-). When both cell types were exposed to 2-MeO-E2, a reduction in the cell viability and an enhancement in the proportions of G2/M cells and apoptotic sub-G1 cells commonly occurred dose-dependently. These 2-MeO-E2-induced cellular changes, except for G2/M arrest, appeared to be more apparent in the presence of p53. Immunofluorescence microscopic anal...
Source: Journal of Microbiology and Biotechnology - September 1, 2014 Category: Biotechnology Authors: Lee JY, Lee SB, Park WY, Choi YJ, Kim B, Kim YH, Jun DY, Kim YH Tags: J Microbiol Biotechnol Source Type: research

Development of Two-Component Nanorod Complex for Dual-Fluorescence Imaging and siRNA delivery.
This study demonstrates the feasibility of using two-component nanorods as a potential theranostic in breast cancer treatment with capabilities in dual imaging and targeted gene delivery. PMID: 24986677 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - July 2, 2014 Category: Biotechnology Authors: Choi JH, Oh BK Tags: J Microbiol Biotechnol Source Type: research

Naringenin exerts cytoprotective effect against Paraquat-induced toxicity in Human Bronchial Epithelial BEAS-2B cells through NRF2 activation.
In this study, we intended to assess the cytoprotective effect of NG against PQ-induced toxicity in human bronchial epithelial BEAS-2B cell line. Co-treatment with NG in PQ-treated BEAS-2B cells can reduce PQ-induced cellular toxicity. NG can also decrease the generation of intracellular ROS caused by PQ treatment. We also observed that treatment with NG in PQ-exposed BEAS-2B cells can significantly induce the expression of antioxidant-related genes, including GPX2, GPX3, GPX5, and GPX7. NG co-treatment can also activate the NRF2 transcription factor and promote its nuclear translocation. In addition, NG co-treatment can i...
Source: Journal of Microbiology and Biotechnology - February 25, 2014 Category: Biotechnology Authors: Podder B, Song HY, Kim YS Tags: J Microbiol Biotechnol Source Type: research

Tristetraprolin regulates prostate cancer cell growth through suppression of E2F1.
Abstract The transcription factor E2F1 is active during the G1 to S transition and is involved in cell cycle and progression. A recent study reported that increased E2F1 is associated with DNA damage and tumour development in several tissues using transgenic models. Here, we show that E2F1 expression is regulated by tristetraprolin (TTP) in prostate cancer. Overexpression of TTP decreased the stability of E2F1 mRNA and the expression level of E2F1. In contrast, inhibition of TTP using siRNA increased the E2F1 expression. E2F1 mRNA contains three AREs within the 3'UTR and TTP destabilized a luciferase mRNA that con...
Source: Journal of Microbiology and Biotechnology - October 22, 2013 Category: Biotechnology Authors: Lee HH, Lee SR, Leem SH Tags: J Microbiol Biotechnol Source Type: research

SIRT1 Suppresses Activating Transcription Factor 4 (ATF4) Expression in Response to Proteasome Inhibition.
Abstract The synthetic machinery of ATF4 (activating transcription factor 4) is activated in response to various stress conditions involved in nutrient restriction, endoplasmic reticulum homeostasis and oxidation. Stress-induced inhibition of proteasome activity triggers the unfolded protein response and endoplasmic reticulum stress where ATF4 is crucial for consequent biological events. In the current study, we showed that the NAD+-dependent deacetylase, SIRT1, suppresses ATF4 synthesis during proteasome inhibition. SIRT1 depletion via transfection of specific siRNA into HeLa cells resulted in a significant incre...
Source: Journal of Microbiology and Biotechnology - October 4, 2013 Category: Biotechnology Authors: Woo SR, Park JE, Kim YH, Ju YJ, Shin HJ, Joo HY, Park ER, Hong SH, Park GH, Lee KH Tags: J Microbiol Biotechnol Source Type: research

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