Histone H3 is digested by Granzyme A during compromised cell death in the Raji cells.

Histone H3 is digested by Granzyme A during compromised cell death in the Raji cells.
J Microbiol Biotechnol. 2015 Jun 2;
Authors: Lee PY, Park BC, Chi SW, Bae KH, Kim S, Cho S, Kim JH, Park SG
Abstract
Granzyme A (GzmA) was identified as a cytotoxic T lymphocyte protease protein expressed at nucleus. A number of nuclear proteins are well known as GzmA substrates, and GzmA is related with caspase independent apoptosis. Histones H1, H2B, and H3 were identified as GzmA substrates through in vitro experiment with purified nucleosome. Here, we demonstrated that histone H3 was cleaved by GzmA in vivo during staurosporine-induced cell death. Moreover, histone H3 cleavage was blocked by the GzmA inhibitor nafamostat mesylate and by GzmA knockdown using siRNA. Taken together we verified that histone H3 is a real substrate for GzmA in vivo in the Raji cells treated by staurosporin.
PMID: 26032366 [PubMed - as supplied by publisher]

Source: Journal of Microbiology and Biotechnology - June 2, 2015 Category: Biotechnology Authors: Lee PY, Park BC, Chi SW, Bae KH, Kim S, Cho S, Kim JH, Park SG Tags: J Microbiol Biotechnol Source Type: research