Filtered By:
Specialty: Pathology
Education: Study

This page shows you your search results in order of date. This is page number 20.

Order by Relevance | Date

Total 491 results found since Jan 2013.

Photoreceptor cell calcium dysregulation and calpain activation promote pathogenic photoreceptor oxidative stress and inflammation in prodromal diabetic retinopathy
In this study, we test the hypothesis that diabetes promotes a greater than normal cytosolic calcium level in rod cells that activates a Ca2+-sensitive protease, calpain, resulting in oxidative stress and inflammation, two pathogenic factors of early diabetic retinopathy (DR). Nondiabetic and 2-months diabetic C57Bl/6J and calpain1 knockout (Capn1-/-) mice were studied; subgroups were treated with a calpain inhibitor (CI). Ca2+ content was measured in photoreceptors using Fura-2. Retinal calpain expression was studied by qTR-PCR and immunohistochemistry. Superoxide and expression of inflammatory proteins were measured usin...
Source: Am J Pathol - July 2, 2021 Category: Pathology Authors: Aicha Saadane Yunpeng Du Wallace B Thoreson Masaru Miyagi Emma M Lessieur Jianying Kiser Xiangyi Wen Bruce A Berkowitz Timothy S Kern Source Type: research

Inhibition of miR-1224 suppresses hypoxia/reoxygenation-induced oxidative stress and apoptosis in cardiomyocytes through targeting GPX4
Exp Mol Pathol. 2021 May 11:104645. doi: 10.1016/j.yexmp.2021.104645. Online ahead of print.ABSTRACTWe have focused on the underlying role of miR-1224 in cardiomyocyte injury stimulated by hypoxia/reoxygenation (H/R). In the current study, the rat cardiomyocyte cell line H9C2 was used to construct a H/R cell model to validate the cardioprotective effects of miR-1224. Data from the dual-luciferase assay revealed that the glutathione peroxidase 4 (GPX4) was a direct target of miR-1224. Expression of miR-1224, determined using qRT-PCR, was remarkably increased while that of GPX4 protein, evaluated via western blotting, was si...
Source: Experimental and Molecular Pathology - May 14, 2021 Category: Pathology Authors: Guibin Li Jiali Jin Shengxin Liu Kejun Ding Caizhen Qian Source Type: research