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Swarms of chemically modified antiviral siRNA targeting herpes simplex virus infection in human corneal epithelial cells
In this study, we show the antiviral efficacy of 2´-fluoro modified antiviral siRNA swarms against HSV-1 in human corneal epithelial cells (HCE). We studied HCE for innate immunity responses to HSV-1, to immunostimulatory cytotoxic double stranded RNA, and to the antiviral siRNA swarms, with or without a viral challenge. The panel of studied innate responses included interferon beta, lambda 1, interferon stimulated gene 54, human myxovirus resistance protein A, human myxovirus resistance protein B, toll-like receptor 3 and interferon kappa. Our results demonstrated that HCE cells are a suitable model to study antiviral RN...
Source: Herpes - July 6, 2022 Category: Infectious Diseases Authors: Kiira Kalke Liisa M Lund Marie C Nyman Alesia A Levanova Arto Urtti Minna M Poranen Veijo Hukkanen Henrik Paavilainen Source Type: research

Viruses, Vol. 12, Pages 1434: Herpes Simplex Virus Type 1 Clinical Isolates Respond to UL29-Targeted siRNA Swarm Treatment Independent of Their Acyclovir Sensitivity
Poranen Veijo Hukkanen Acyclovir is the drug of choice for the treatment of herpes simplex virus (HSV) infections. Acyclovir-resistant HSV strains may emerge, especially during long-term drug use, and subsequently cause difficult-to-treat exacerbations. Previously, we set up a novel treatment approach, based on enzymatically synthesized pools of siRNAs, or siRNA swarms. These swarms can cover kilobases-long target sequences, reducing the likelihood of resistance to treatment. Swarms targeting the UL29 essential gene of HSV-1 have demonstrated high efficacy against HSV-1 in vitro and in vivo. Here, we assessed the an...
Source: Viruses - December 13, 2020 Category: Virology Authors: Kiira Kalke Jenni Lehtinen Jelena Gnjatovic Liisa M. Lund Marie C. Nyman Henrik Paavilainen Julius Orpana Tuomas Lasanen Fanny Frejborg Alesia A. Levanova Tytti Vuorinen Minna M. Poranen Veijo Hukkanen Tags: Article Source Type: research