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17 β-Estradiol activates Cl < sup > - < /sup > channels via the estrogen receptor α pathway in human thyroid cells
In this study, the effects of estrogen on chloride channel activities in human thyroid Nthy-ori3-1 cells were therefore investigated using the whole cell patch-clamp technique. The results showed that the extracellular application of 17β-estradiol (E2) activated Cl- currents, which reversed at a potential close to Cl- equilibrium potential and showed remarkable outward rectification and an anion permeability of I- > Br- > Cl- > gluconate. The Cl- currents were inhibited by the chloride channel blockers, NPPB and tamoxifen. Quantitative Real-time PCR results demonstrated that ClC-3 expression was highest in ClC fa...
Source: Channels - August 20, 2021 Category: Molecular Biology Authors: Meisheng Yu Yuan Wei Yanfang Zheng Lili Yang Long Meng Jiawei Lin Peisheng Xu Sanaa Ahmed Nagi Abdu Mahdy Linyan Zhu Shuang Peng Lixin Chen Liwei Wang Source Type: research

TMEM16A channel upregulation in arterial smooth muscle cells produces vasoconstriction during diabetes.
Abstract The pathological involvement of anion channels in vascular dysfunction that occurs during type 2 diabetes (T2D) is unclear. Here, we tested the hypothesis that TMEM16A, a calcium (Ca2+)-activated chloride (Cl-) channel, contributes to modifications in arterial contractility during T2D. Our data indicate that T2D increased TMEM16A mRNA in arterial smooth muscle cells (myocytes) and total and surface TMEM16A protein in resistance-size arteries of mice. To examine vascular cell types in which TMEM16A expression is upregulated, we generated a tamoxifen-inducible, smooth muscle-specific TMEM16A knockout (TMEM1...
Source: American Journal of Physiology. Heart and Circulatory Physiology - January 15, 2021 Category: Physiology Authors: Leo MD, Peixoto-Neves D, Yin W, Raghavan S, Muralidharan P, Mata-Daboin A, Jaggar JH Tags: Am J Physiol Heart Circ Physiol Source Type: research