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Canis familiaris allergen Can f 7: Expression, purification and analysis of B cell epitopes in Chinese children with dog allergies.
Abstract Dogs are a major source of indoor allergens. However, the prevalence of dog allergies in China remains unclear, especially in children. In the present study, Can f 7, a canine allergen belonging to the Niemann pick type C2 protein family, was selected to study its sensitization rate in Chinese children with dog allergies. The Can f 7 gene was subcloned into a pET‑28a vector and expressed in Escherichia coli BL21 (DE3) cells. Recombinant Can f 7 was purified by nickel affinity chromatography, identified by SDS‑PAGE electrophoresis, and had its allergenicity assessed by western blot, ELISA and basophil ...
Source: International Journal of Molecular Medicine - January 15, 2019 Category: Molecular Biology Authors: Wang RQ, Wang YJ, Xu ZQ, Zhou YJ, Cao MD, Zhu W, Sun JL, Wei JF Tags: Int J Mol Med Source Type: research

Nontyphoidal Salmonella Gastroenteritis in a Tertiary Children's Hospital in Southern China: Characteristics and Dietary Considerations.
Conclusions: Salmonella Typhimurium was the predominant serotype. Most patients with nontyphoidal Salmonella gastroenteritis were younger than 3 years old. Lactose intolerance occurred frequently in children with nontyphoidal Salmonella gastroenteritis and dietary modification should be considered when diarrhea is persistent and prolonged. PMID: 29686701 [PubMed]
Source: Gastroenterology Research and Practice - April 26, 2018 Category: Gastroenterology Tags: Gastroenterol Res Pract Source Type: research