Genetic diversity, symbiotic efficiency, stress tolerance, and plant growth promotion traits of rhizobia nodulating Vachellia tortilis subsp. raddiana growing in dryland soils in southern Morocco
Syst Appl Microbiol. 2023 May 15;46(4):126434. doi: 10.1016/j.syapm.2023.126434. Online ahead of print.ABSTRACTIn the present study, we analyzed the genetic diversity, phylogenetic relationships, stress tolerance, phytobeneficial traits, and symbiotic characteristics of rhizobial strains isolated from root nodules of Vachellia tortilis subsp. raddiana grown in soils collected in the extreme Southwest of the Anti-Atlas Mountains in Morocco. Subsequent to Rep-PCR fingerprinting, 16S rDNA gene sequencing of 15 representative strains showed that all of them belong to the genus Ensifer. Phylogenetic analysis and concatenation o...
Source: Systematic and Applied Microbiology - May 21, 2023 Category: Microbiology Authors: Mohamed Hnini Imane El Attar Kaoutar Taha Jamal Aurag Source Type: research

Genetic diversity, symbiotic efficiency, stress tolerance, and plant growth promotion traits of rhizobia nodulating Vachellia tortilis subsp. raddiana growing in dryland soils in southern Morocco
Syst Appl Microbiol. 2023 May 15;46(4):126434. doi: 10.1016/j.syapm.2023.126434. Online ahead of print.ABSTRACTIn the present study, we analyzed the genetic diversity, phylogenetic relationships, stress tolerance, phytobeneficial traits, and symbiotic characteristics of rhizobial strains isolated from root nodules of Vachellia tortilis subsp. raddiana grown in soils collected in the extreme Southwest of the Anti-Atlas Mountains in Morocco. Subsequent to Rep-PCR fingerprinting, 16S rDNA gene sequencing of 15 representative strains showed that all of them belong to the genus Ensifer. Phylogenetic analysis and concatenation o...
Source: Systematic and Applied Microbiology - May 21, 2023 Category: Microbiology Authors: Mohamed Hnini Imane El Attar Kaoutar Taha Jamal Aurag Source Type: research

Genetic diversity, symbiotic efficiency, stress tolerance, and plant growth promotion traits of rhizobia nodulating Vachellia tortilis subsp. raddiana growing in dryland soils in southern Morocco
Syst Appl Microbiol. 2023 May 15;46(4):126434. doi: 10.1016/j.syapm.2023.126434. Online ahead of print.ABSTRACTIn the present study, we analyzed the genetic diversity, phylogenetic relationships, stress tolerance, phytobeneficial traits, and symbiotic characteristics of rhizobial strains isolated from root nodules of Vachellia tortilis subsp. raddiana grown in soils collected in the extreme Southwest of the Anti-Atlas Mountains in Morocco. Subsequent to Rep-PCR fingerprinting, 16S rDNA gene sequencing of 15 representative strains showed that all of them belong to the genus Ensifer. Phylogenetic analysis and concatenation o...
Source: Systematic and Applied Microbiology - May 21, 2023 Category: Microbiology Authors: Mohamed Hnini Imane El Attar Kaoutar Taha Jamal Aurag Source Type: research

Novel symbiovars ingae, lysilomae and lysilomaefficiens in bradyrhizobia from tree-legume nodules
Syst Appl Microbiol. 2023 May 11;46(4):126433. doi: 10.1016/j.syapm.2023.126433. Online ahead of print.ABSTRACTInga vera and Lysiloma tree legumes form nodules with Bradyrhizobium spp. from the japonicum group that represent novel genomospecies, for which we describe here using genome data, symbiovars lysilomae, lysilomaefficiens and ingae. Genes encoding Type three secretion system (TTSS) that could affect host specificity were found in ingae but not in lysilomae nor in lysilomaefficiens symbiovars and uptake hydrogenase hup genes (that affect nitrogen fixation) were observed in bradyrhizobia from the symbiovars ingae and...
Source: Systematic and Applied Microbiology - May 19, 2023 Category: Microbiology Authors: Diana Hern ández-Oaxaca Karen Claro Marco A Rogel M ónica Rosenblueth Julio Martinez-Romero Esperanza Martinez-Romero Source Type: research

Novel symbiovars ingae, lysilomae and lysilomaefficiens in bradyrhizobia from tree-legume nodules
Syst Appl Microbiol. 2023 May 11;46(4):126433. doi: 10.1016/j.syapm.2023.126433. Online ahead of print.ABSTRACTInga vera and Lysiloma tree legumes form nodules with Bradyrhizobium spp. from the japonicum group that represent novel genomospecies, for which we describe here using genome data, symbiovars lysilomae, lysilomaefficiens and ingae. Genes encoding Type three secretion system (TTSS) that could affect host specificity were found in ingae but not in lysilomae nor in lysilomaefficiens symbiovars and uptake hydrogenase hup genes (that affect nitrogen fixation) were observed in bradyrhizobia from the symbiovars ingae and...
Source: Systematic and Applied Microbiology - May 19, 2023 Category: Microbiology Authors: Diana Hern ández-Oaxaca Karen Claro Marco A Rogel M ónica Rosenblueth Julio Martinez-Romero Esperanza Martinez-Romero Source Type: research

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
Syst Appl Microbiol. 2023 Apr 26;46(4):126424. doi: 10.1016/j.syapm.2023.126424. Online ahead of print.ABSTRACTA polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence ana...
Source: Systematic and Applied Microbiology - May 11, 2023 Category: Microbiology Authors: Dana Nov áková Vendula Koublov á Karel Sedl ář Eva Sta ňková Stanislava Kr álová Pavel Švec Meina Neumann-Schaal Jacqueline Wolf Sylva Koudelkov á Milo š Barták Ivo Sedl áček Source Type: research

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
Syst Appl Microbiol. 2023 Apr 26;46(4):126424. doi: 10.1016/j.syapm.2023.126424. Online ahead of print.ABSTRACTA polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence ana...
Source: Systematic and Applied Microbiology - May 11, 2023 Category: Microbiology Authors: Dana Nov áková Vendula Koublov á Karel Sedl ář Eva Sta ňková Stanislava Kr álová Pavel Švec Meina Neumann-Schaal Jacqueline Wolf Sylva Koudelkov á Milo š Barták Ivo Sedl áček Source Type: research

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
Syst Appl Microbiol. 2023 Apr 26;46(4):126424. doi: 10.1016/j.syapm.2023.126424. Online ahead of print.ABSTRACTA polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence ana...
Source: Systematic and Applied Microbiology - May 11, 2023 Category: Microbiology Authors: Dana Nov áková Vendula Koublov á Karel Sedl ář Eva Sta ňková Stanislava Kr álová Pavel Švec Meina Neumann-Schaal Jacqueline Wolf Sylva Koudelkov á Milo š Barták Ivo Sedl áček Source Type: research

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
Syst Appl Microbiol. 2023 Apr 26;46(4):126424. doi: 10.1016/j.syapm.2023.126424. Online ahead of print.ABSTRACTA polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence ana...
Source: Systematic and Applied Microbiology - May 11, 2023 Category: Microbiology Authors: Dana Nov áková Vendula Koublov á Karel Sedl ář Eva Sta ňková Stanislava Kr álová Pavel Švec Meina Neumann-Schaal Jacqueline Wolf Sylva Koudelkov á Milo š Barták Ivo Sedl áček Source Type: research

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
Syst Appl Microbiol. 2023 Apr 26;46(4):126424. doi: 10.1016/j.syapm.2023.126424. Online ahead of print.ABSTRACTA polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence ana...
Source: Systematic and Applied Microbiology - May 11, 2023 Category: Microbiology Authors: Dana Nov áková Vendula Koublov á Karel Sedl ář Eva Sta ňková Stanislava Kr álová Pavel Švec Meina Neumann-Schaal Jacqueline Wolf Sylva Koudelkov á Milo š Barták Ivo Sedl áček Source Type: research

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
Syst Appl Microbiol. 2023 Apr 26;46(4):126424. doi: 10.1016/j.syapm.2023.126424. Online ahead of print.ABSTRACTA polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence ana...
Source: Systematic and Applied Microbiology - May 11, 2023 Category: Microbiology Authors: Dana Nov áková Vendula Koublov á Karel Sedl ář Eva Sta ňková Stanislava Kr álová Pavel Švec Meina Neumann-Schaal Jacqueline Wolf Sylva Koudelkov á Milo š Barták Ivo Sedl áček Source Type: research

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
Syst Appl Microbiol. 2023 Apr 26;46(4):126424. doi: 10.1016/j.syapm.2023.126424. Online ahead of print.ABSTRACTA polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence ana...
Source: Systematic and Applied Microbiology - May 11, 2023 Category: Microbiology Authors: Dana Nov áková Vendula Koublov á Karel Sedl ář Eva Sta ňková Stanislava Kr álová Pavel Švec Meina Neumann-Schaal Jacqueline Wolf Sylva Koudelkov á Milo š Barták Ivo Sedl áček Source Type: research

Description of Dellaglioa carnosa sp. nov., a novel species isolated from high-oxygen modified-atmosphere packaged meat
Syst Appl Microbiol. 2023 Apr 28;46(4):126423. doi: 10.1016/j.syapm.2023.126423. Online ahead of print.ABSTRACTThe study provides a taxonomic characterization of three bacterial strains isolated from high-oxygen modified-atmosphere packaged beef from Germany. The strains of the novel species shared identical 16S rRNA gene sequence to the closely related type strain of Dellaglioa algida. However, the in-silico DNA-DNA hybridization (DDH) values indicate that they belong to a different genomic species. The in silico DDH estimate value between TMW 2.2523T and the type strain of Dellaglioa algida DSM 15638T was only 63.2 %. Th...
Source: Systematic and Applied Microbiology - May 6, 2023 Category: Microbiology Authors: Victoria Werum Matthias Ehrmann Source Type: research

Description of Dellaglioa carnosa sp. nov., a novel species isolated from high-oxygen modified-atmosphere packaged meat
Syst Appl Microbiol. 2023 Apr 28;46(4):126423. doi: 10.1016/j.syapm.2023.126423. Online ahead of print.ABSTRACTThe study provides a taxonomic characterization of three bacterial strains isolated from high-oxygen modified-atmosphere packaged beef from Germany. The strains of the novel species shared identical 16S rRNA gene sequence to the closely related type strain of Dellaglioa algida. However, the in-silico DNA-DNA hybridization (DDH) values indicate that they belong to a different genomic species. The in silico DDH estimate value between TMW 2.2523T and the type strain of Dellaglioa algida DSM 15638T was only 63.2 %. Th...
Source: Systematic and Applied Microbiology - May 6, 2023 Category: Microbiology Authors: Victoria Werum Matthias Ehrmann Source Type: research

Description of Dellaglioa carnosa sp. nov., a novel species isolated from high-oxygen modified-atmosphere packaged meat
Syst Appl Microbiol. 2023 Apr 28;46(4):126423. doi: 10.1016/j.syapm.2023.126423. Online ahead of print.ABSTRACTThe study provides a taxonomic characterization of three bacterial strains isolated from high-oxygen modified-atmosphere packaged beef from Germany. The strains of the novel species shared identical 16S rRNA gene sequence to the closely related type strain of Dellaglioa algida. However, the in-silico DNA-DNA hybridization (DDH) values indicate that they belong to a different genomic species. The in silico DDH estimate value between TMW 2.2523T and the type strain of Dellaglioa algida DSM 15638T was only 63.2 %. Th...
Source: Systematic and Applied Microbiology - May 6, 2023 Category: Microbiology Authors: Victoria Werum Matthias Ehrmann Source Type: research