Role of stem cells in fertility preservation: current insights.
Authors: Vermeulen M, Giudice MG, Del Vento F, Wyns C Abstract While improvements made in the field of cancer therapy allow high survival rates, gonadotoxicity of chemo- and radiotherapy can lead to infertility in male and female pre- and postpubertal patients. Clinical options to preserve fertility before starting gonadotoxic therapies by cryopreserving sperm or oocytes for future use with assisted reproductive technology (ART) are now applied worldwide. Cryopreservation of pre- and postpubertal ovarian tissue containing primordial follicles, though still considered experimental, has already led to the birth of he...
Source: Stem Cells and Cloning: Advances and Applications - September 10, 2019 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Stem-cell therapy for ovariectomy-induced osteoporosis in rats: a comparison of three treatment modalities.
This study compares the influence of MSCs, osteoblasts, and exosomes derived from osteoblasts for the treatment of osteoporosis. Methods: Osteoporosis was induced in 40 female Sprague Dawley rats by performing ovariectomy. After 12 weeks, bone marrow was harvested and MSCs separated from bone-marrow aspirate as described by Piao et al. After 15 days, autologous osteogenically differentiated cells from the MSCs were available. Exosomes were isolated from osteoblasts by modification of the technique described by Ge et al. MSCs and osteoblasts (106 cells in 0.5 mL normal saline) and exosomes (100 µg protein) were inject...
Source: Stem Cells and Cloning: Advances and Applications - July 31, 2019 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Treatment of osteonecrosis of the femoral head by core decompression and implantation of fully functional ex vivo-expanded bone marrow-derived mesenchymal stem cells: a proof-of-concept study.
Authors: Mardones R, Camacho D, Monsalvo F, Zulch N, Jofre C, Minguell JJ Abstract Background: Based on several attributes involved in bone formation, bone marrow-resident mesenchymal stem cells (MSCs) have been employed in the treatment of patients suffering from femoral head osteonecrosis. Due to the low content of MSCs in the bone marrow, ex vivo expansion procedures are utilized to increase the cell number. Customarily, before administration of the resulting expanded cell product MSCs to the patient, its cellular identity is usually evaluated according to a set of "minimal phenotypic" markers, which a...
Source: Stem Cells and Cloning: Advances and Applications - March 20, 2019 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Simplified in vitro engineering of neuromuscular junctions between rat embryonic motoneurons and immortalized human skeletal muscle cells.
Conclusion: This coculture system provides a tool to study vital features of NMJ formation, regulation, maintenance, and repair, as well as a model platform to explore neuromuscular diseases and disorders affecting NMJs. PMID: 30863121 [PubMed] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - March 15, 2019 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Stem cells for luminal, fistulizing, and perianal inflammatory bowel disease: a comprehensive updated review of the literature.
Authors: Turse EP, Dailey FE, Naseer M, Partyka EK, Bragg JD, Tahan V Abstract Much research has been performed over the last decade on stem cell therapy as treatment for patients with inflammatory bowel disease. Hematopoietic and mesenchymal stem cells, both allogeneic (from someone else) and autologous (from own patient), have been studied with safe and efficacious results in the majority of patients treated for luminal, perianal, and/or fistulizing disease. Here in this review, we highlight all human trials that have been conducted utilizing stem cell therapy treatment in patients with inflammatory bowel disease...
Source: Stem Cells and Cloning: Advances and Applications - December 22, 2018 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

A functional human motor unit platform engineered from human embryonic stem cells and immortalized skeletal myoblasts.
Conclusion: We established a functional human motor unit platform for in vitro investigations. Thus, this co-culture system can be used as a novel platform for 1) drug discovery in the treatment of neuromuscular disorders, 2) deciphering vital features of NMJ formation, regulation, maintenance, and repair, and 3) exploring neuromuscular diseases, age-associated degeneration of the NMJ, muscle aging, and diabetic neuropathy and myopathy. PMID: 30519053 [PubMed] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - December 8, 2018 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Bone marrow-derived mesenchymal stromal cell: what next?
Authors: Borges FT, Convento MB, Schor N Abstract Bone marrow mesenchymal stromal cell (MSC) is a potential alternative in regenerative medicine and has great potential in many pathologic conditions including kidney disease. Although most of the studies demonstrate MSC efficiency, the regenerative potential may not be efficient in all diseases and patients. Stem cell feasibility is modified by donor characteristics as gender, age, diet, and health status, producing both positive and negative results. The conditioning of MSC can potentiate its effects and modify its culture medium (CM). In current practices, the cel...
Source: Stem Cells and Cloning: Advances and Applications - December 5, 2018 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Use of paracrine factors from stem cells to treat local radiation burns in rats.
Conclusion: In the control group, the area of the lesion ranged from 1.4±0.6 cm2 on the 50th day to 1.9±0.8 cm2 on the 71st day. During the 57th to the 71st day, the difference between the affected area in the experimental and control groups was 1:8. The experimental group has a significantly higher level of skin regeneration and significant decrease in the level of leukocyte infiltration, thereby reducing necrosis. PMID: 30464536 [PubMed] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - November 24, 2018 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research.
Conclusion: The distinct features of rASCs and hASCs can positively or negatively affect their use for different applications in biotechnology (such as cell reprogramming) and translational studies (such as cell transplantation, tissue engineering, and pharmacokinetics). Nevertheless, the particularities between rabbit and human MSCs should not prevent rabbit use in preclinical models, but care should be taken to interpret results and properly translate animal findings to medicine. PMID: 30425533 [PubMed] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - November 15, 2018 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Stem cell therapy in autism: recent insights.
Authors: Siniscalco D, Kannan S, Semprún-Hernández N, Eshraghi AA, Brigida AL, Antonucci N Abstract Autism spectrum disorders (ASDs) are characterized by core domains: persistent deficits in social communication and interaction; restricted, repetitive patterns of behavior, interests, or activities. ASDs comprise heterogeneous and complex neurodevelopmental pathologies with well-defined inflammatory conditions and immune system dysfunction. Due to neurobiologic changes underlying ASD development, cell-based therapies have been proposed and applied to ASDs. Indeed, stem cells show specific immunologic p...
Source: Stem Cells and Cloning: Advances and Applications - November 15, 2018 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Sirt1 protects neural stem cells from apoptosis by decreasing acetylation of histone 3K9.
Conclusion: Sirt1 may protect NSCs from apoptosis by decreasing the acetylation of histone 3 on K9. PMID: 30233218 [PubMed] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - September 22, 2018 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Cryostorage of immature and mature human testis tissue to preserve spermatogonial stem cells (SSCs): a systematic review of current experiences toward clinical applications.
Authors: Zarandi NP, Galdon G, Kogan S, Atala A, Sadri-Ardekani H Abstract While the survival rate of children with cancer is increasing, preserving fertility for prepubertal boys is still a challenge. Although intracytoplasmic sperm injection (ICSI) using frozen sperms has revolutionized infertility treatment, it is not applicable for the patients who undergo chemotherapy before puberty since spermatogenesis has not begun. Therefore, preserving spermatogonial stem cells (SSCs) as an experimental option can be provided to prepubertal patients at a risk of damage or loss of their SSCs due to cancer treatments and de...
Source: Stem Cells and Cloning: Advances and Applications - July 18, 2018 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses.
In this study, we evaluated the use of bone marrow-MSCs (BM-MSCs) as nuclear donors in horse cloning, and we compared the in vitro and in vivo embryo development with respect to fibroblasts. Materials and methods: Zona-free nuclear transfer was performed using BM-MSCs (MSC group, n=3432) or adult fibroblasts (AF group, n=4527). Embryos produced by artificial insemination (AI) recovered by uterine flushing and transferred to recipient mares were used as controls (AI group). Results: Blastocyst development was higher in the MSC group than in the AF group (18.1% vs 10.9%, respectively; p
Source: Stem Cells and Cloning: Advances and Applications - March 3, 2018 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

A review on stem cell therapy for multiple sclerosis: special focus on human embryonic stem cells.
In this study, an overview of the current knowledge about the unique properties of hESC and their comparison with other cell therapies has been presented for the treatment of patients with MS. PMID: 29483778 [PubMed] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - March 1, 2018 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Expression profiling of adhesion proteins during prenatal and postnatal liver development in rats.
Authors: Yousaf M, Tayyeb A, Ali G Abstract Culturing of primary hepatocytes and stem cell-derived hepatocytes faces a major issue of dedifferentiation due to absence of cell-cell adhesion and 3D structures. One of the possible ways to eliminate the problem of dedifferentiation is mimicking the expression pattern of adhesion proteins during the normal developmental process of liver cells. The purpose of this study was to evaluate the expression pattern of some key adhesion proteins, namely, E-cadherin, N-cadherin, epithelial CAM (EpCAM), intracellular CAM (ICAM), collagen 1α1, α-actinin, β-catenin ...
Source: Stem Cells and Cloning: Advances and Applications - October 17, 2017 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Conventional and novel stem cell based therapies for androgenic alopecia.
Authors: Talavera-Adame D, Newman D, Newman N Abstract The prevalence of androgenic alopecia (AGA) increases with age and it affects both men and women. Patients diagnosed with AGA may experience decreased quality of life, depression, and feel self-conscious. There are a variety of therapeutic options ranging from prescription drugs to non-prescription medications. Currently, AGA involves an annual global market revenue of US$4 billion and a growth rate of 1.8%, indicating a growing consumer market. Although natural and synthetic ingredients can promote hair growth and, therefore, be useful to treat AGA, some of th...
Source: Stem Cells and Cloning: Advances and Applications - October 6, 2017 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Hair follicle growth by stromal vascular fraction-enhanced adipose transplantation in baldness.
Authors: Perez-Meza D, Ziering C, Sforza M, Krishnan G, Ball E, Daniels E Abstract Great interest remains in finding new and emerging therapies for the treatment of male and female pattern hair loss. The autologous fat grafting technique is>100 years old, with a recent and dramatic increase in clinical experience over the past 10-15 years. Recently, in 2001, Zuk et al published the presence of adipose-derived stem cells, and abundant research has shown that adipose is a complex, biological active, and important tissue. Festa et al, in 2011, reported that adipocyte lineage cells support the stem cell niche and he...
Source: Stem Cells and Cloning: Advances and Applications - July 27, 2017 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Stem cell technology in breast cancer: current status and potential applications.
Authors: Chiotaki R, Polioudaki H, Theodoropoulos PA Abstract Breast cancer, the leading cause of cancer among females, is supported by the presence of a rare subset of undifferentiated cells within the tumor, identified as breast cancer stem cells (BCSCs). BCSCs underlie the mechanisms of tumor initiation and sustenance and are implicated in the dissemination of the primary tumor to metastatic sites, as they have been found circulating in the blood of breast cancer patients. The discovery of BCSCs has generated a great amount of interest among the scientific community toward their isolation, molecular characteriza...
Source: Stem Cells and Cloning: Advances and Applications - May 26, 2016 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells.
This study was able to characterize NSCs in culture under differentiation for neurogenesis in the pathway of the neural differentiation process by studying the expressed neural genes and the ability to maintain these NSCs in culture for further differentiation in thousands of functional neurons for the treatment of brain and spinal cord injuries and defects. PMID: 27143939 [PubMed] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - May 6, 2016 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Stem cell technology for tendon regeneration: current status, challenges, and future research directions.
Authors: Lui PP Abstract Tendon injuries are a common cause of physical disability. They present a clinical challenge to orthopedic surgeons because injured tendons respond poorly to current treatments without tissue regeneration and the time required for rehabilitation is long. New treatment options are required. Stem cell-based therapies offer great potential to promote tendon regeneration due to their high proliferative, synthetic, and immunomodulatory activities as well as their potential to differentiate to the target cell types and undergo genetic modification. In this review, I first recapped the challenges ...
Source: Stem Cells and Cloning: Advances and Applications - January 12, 2016 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Defining the expression of marker genes in equine mesenchymal stromal cells.
Authors: Guest DJ, Ousey JC, Smith MR Abstract Mesenchymal stromal (MS) cells have been derived from multiple sources in the horse including bone marrow, adipose tissue and umbilical cord blood. To date these cells have been investigated for their differentiation potential and are currently being used to treat damage to horse musculoskeletal tissues. However, no work has been done in horse MS cells to examine the expression profile of proteins and cell surface antigens that are expressed in human MS cells. The identification of such profiles in the horse will allow the comparison of putative MS cells isolated from ...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Epigenetic modifications of embryonic stem cells: current trends and relevance in developing regenerative medicine.
Authors: Chung H, Sidhu KS Abstract Epigenetics is a growing field not only in the area of cancer research but recently in stem cells including human embryonic stem cell (hESC) research. The hallmark of profiling epigenetic changes in stem cells lies in maintaining pluripotency or multipotency and in attaining lineage specifications that are relevant for regenerative medicine. Epigenetic modifications including DNA methylation, histone acetylation and methylation, play important roles in regulating gene expressions. Other epigenetic modifications include X chromosome silencing, genomic stability and imprinting and ...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Regeneration and adult stem cells in the human female reproductive tract.
Authors: Ono M, Maruyama T, Yoshimura Y Abstract The human uterus is unique in that it exhibits a tremendous regenerative capacity that enables cyclical regeneration and remodeling throughout a woman's reproductive life. This plasticity of the reproductive system has recently been highlighted. Regeneration and remodeling in the female reproductive tract alludes to the existence of endometrial and myometrial stem cell systems, which has been supported by increasing experimental evidence. Characterization of these stem cells, along with the study of the mechanisms controlling their regeneration, will improve the unde...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Stem cell horizons in intervertebral disc degeneration.
Authors: Ciacci J, Ho A, Ames CP, Jandial R Abstract Intervertebral disc degeneration remains a pervasive and intractable disease arising from a combination of aging and stress on the back and spine. The growing field of regenerative medicine brings the promise of stem cells in the treatment of disc disease. Scientists and physicians hope to employ stem cells not only to stop, but also reverse degeneration. However, there are many important outstanding issues, including the hostile avascular, apoptotic physiological environment of the intervertebral disc, and the difficulty of obtaining mesenchymal stem cells, and ...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Stem cells and the origin of gliomas: A historical reappraisal with molecular advancements.
Authors: Levy ML, Ho AL, Hughes S, Menon J, Jandial R Abstract The biology of both normal and tumor development clearly possesses overlapping and parallel features. Oncogenes and tumor suppressors are relevant not only in tumor biology, but also in physiological developmental regulators of growth and differentiation. Conversely, genes identified as regulators of developmental biology are relevant to tumor biology. This is particularly relevant in the context of brain tumors, where recent evidence is mounting that the origin of brain tumors, specifically gliomas, may represent dysfunctional developmental neurobiolog...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Treatment of systemic sclerosis: potential role for stem cell transplantation.
Authors: Xiong W, Derk CT Abstract Hematopoietic stem cell transplantation may "reset" the immune reconstitution and induce self tolerance of autoreactive lymphocytes, and has been explored in the treatments for systemic sclerosis. Phase I/II trials have shown a satisfactory risk benefit ratio. The true benefit will be identified by two ongoing prospective, randomized phase III trials. Multipotent mesenchymal stromal cells (MSCs) possess antiproliferative, anti-inflammatory, and immunosuppressive properties. The use of MSCs has showed successful responses in patients with severe steroid-resistant acute gr...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Bone marrow mononuclear stem cells: potential in the treatment of myocardial infarction.
Authors: Leblond AL, O'Sullivan J, Caplice N Abstract Despite advances in the management of myocardial infarction, congestive heart failure following myocardial infarction continues to be a major worldwide medical problem. Mononuclear cells from bone marrow are currently being studied as potential candidates for cell-based therapy to repair and regenerate damaged myocardium, with mixed results. The success of this strategy requires structural repair through both cardiomyogenesis and angiogenesis but also functional repair. However, pre-clinical and clinical studies with the intracoronary administration of cells ind...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Development of gene therapy: potential in severe combined immunodeficiency due to adenosine deaminase deficiency.
Authors: Montiel-Equihua CA, Thrasher AJ, Gaspar HB Abstract The history of stem cell gene therapy is strongly linked to the development of gene therapy for severe combined immunodeficiencies (SCID) and especially adenosine deaminase (ADA)-deficient SCID. Here we discuss the developments achieved in over two decades of clinical and laboratory research that led to the establishment of a protocol for the autologous transplant of retroviral vector-mediated gene-modified hematopoietic stem cells, which has proved to be both successful and, to date, safe. Patients in trials in three different countries have shown long-t...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Current protocols in the generation of pluripotent stem cells: theoretical, methodological and clinical considerations.
Authors: Swelstad BB, Kerr CL Abstract Pluripotent stem cells have been derived from various embryonic, fetal and adult sources. Embryonic stem cells (ESCs) and parthenogenic ESCs (pESCs) are derived from the embryo proper while embryonic germ cells (EGCs), embryonal carcinoma cells (ECCs), and germ-line stem cells (GSC) are produced from germ cells. ECCs were the first pluripotent stem cell lines established from adult testicular tumors while EGCs are generated in vitro from primordial germ cells (PGCs) isolated in late embryonic development. More recently, studies have also demonstrated the ability to produce GSC...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Hepatic progenitors for liver disease: current position.
Authors: Conigliaro A, Brenner DA, Kisseleva T Abstract Liver regeneration restores the original functionality of hepatocytes and cholangiocytes in response to injury. It is regulated on several levels, with different cellular populations contributing to this process, eg, hepatocytes, liver precursor cells, intrahepatic stem cells. In response to injury, mature hepatocytes have the capability to proliferate and give rise to new hepatocytes and cholangiocytes. Meanwhile, liver precursor cells (oval cells) have become the most recognized bipotential precursor cells in the damaged liver. They rapidly proliferate, chan...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Potential and clinical utility of stem cells in cardiovascular disease.
Authors: Krause K, Schneider C, Jaquet K, Kuck KH Abstract The recent identification of bone marrow-derived adult stem cells and other types of stem cells that could improve heart function after transplantation have raised high expectations. The basic mechanisms have been studied mostly in murine models. However, these experiments revealed controversial results on transdifferentiation vs transfusion of adult stem cells vs paracrine effects of these cells, which is still being debated. Moreover, the reproducibility of these results in precisely translated large animal models is still less well investigated. Despite ...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Hematopoietic stem cells: ex-vivo expansion and therapeutic potential for myocardial ischemia.
Authors: Lu J, Pompili VJ, Das H Abstract Despite recent advances in cardiovascular medicine, ischemic heart disease remains the major cause of death in the United States and abroad. Cell-based therapy for degenerative diseases like myocardial ischemia using stem cells is currently under serious investigation. Various types of stem cells are being considered to be candidates for cell transplantation in cell-based therapy. Hematopoietic stem cells are one of the most promising cell types as several studies demonstrated their ability to improve ischemic cardiac functions by enhancing neovascularization and by reducin...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Hematopoietic stem cell transplantation for the management of follicular lymphoma.
Authors: Hosing C Abstract Although much has been published on the application of autologous and allogeneic hematopoietic stem cell transplantation in patients with follicular lymphoma (FL), no uniform consensus exists among physicians on when to use this strategy. Three large randomized trials failed to show a survival benefit using autologous transplantation for FL patients in first complete remission. Similarly, many Phase II or registry-based studies have also failed to show a survival benefit with autologous transplantation in relapsed or refractory FL patients, although the progression-free survival seems to ...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Progress in stem cell-derived technologies for hepatocellular carcinoma.
Authors: Aravalli RN Abstract Primary hepatocellular carcinoma (HCC) is a common malignancy that has a poor prognosis because it is often diagnosed at an advanced stage. HCC normally develops as a consequence of underlying liver disease and is most often associated with cirrhosis. Surgical resection and liver transplantation are the current best options to treat liver cancer. However, problems associated with liver transplantation, such as shortage of donors, risk of immune rejection, and tissue damage following surgery provided the impetus for development of alternative therapies. The emerging field of stem cell t...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Tackling mantle cell lymphoma (MCL): Potential benefit of allogeneic stem cell transplantation.
Authors: Shanbhag S, Smith MR, Emmons RV Abstract Mantle cell lymphoma (MCL) is a type of non-Hodgkins lymphoma (NHL) associated with poor progression-free and overall survival. There is a high relapse rate with conventional cytotoxic chemotherapy. Intensive combination chemotherapy including rituximab, dose intense CHOP- (cyclophosphamide-doxorubicin-vincristine-prednisone) like regimens, high dose cytarabine, and/or consolidation with autologous stem cell transplant (autoSCT) have shown promise in significantly prolonging remissions. Data from phase II studies show that even in patients with chemotherapy refracto...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Are reviewers obstructing stem cell research?
Authors: Binetruy B PMID: 24198515 [PubMed] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Hematopoietic stem cell transplantation.
This article reviews the biology of stem cells, clinical efficacy of HSCT, transplantation procedures, and potential complications. PMID: 24198516 [PubMed] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Aging differentially affects male and female neural stem cell neurogenic properties.
CONCLUSION: We provided in vitro evidence that the fate of neural stem cells is affected by sex and aging. Indeed, young male neural stem cells mainly expressed markers of neuronal and oligodendroglial fate, whereas young female neural stem cells underwent differentiation towards an astroglial phenotype. Aging resulted in a lessened capacity to express neuron and astrocyte markers. Undifferentiated neural stem cells displayed sexual dimorphism in the expression of steroid receptors, in particular ERα and ERβ, and the expression level of several steroid receptors increased during aging. Such sexual dimorphism mig...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Gene transfection in primary stem-like cells of giant cell tumor of bone.
Authors: Singh S, Mak I, Power P, Cunningham M, Cunnigham M, Turcotte R, Ghert M Abstract The neoplastic stem-like stromal cell of giant cell tumor of bone (GCT) survives for multiple passages in primary culture with a stable phenotype, and exhibits multipotent characteristics. The pathophysiology of this tumor has been studied through the primary culture of these cells. However, successful gene transfer of these cells has not been reported to date. In this short report, we describe the development of the first reported technique that results in efficient gene transfection in primary stem-like cells of GCT. PM...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Delivered growth factor therapy to improve healing after rotator cuff repair.
CONCLUSIONS: Rotator cuff repair can benefit from biologic augmentation. However, research in this field is still young and has not yet demonstrated that the benefits in healing rates are significant enough to merit regular clinical use. Randomized controlled trials will elucidate the use of biologic augmentation in rotator cuff repairs. PMID: 24198519 [PubMed] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Amyotrophic lateral sclerosis: applications of stem cells - an update.
Authors: Cova L, Silani V Abstract Neurodegenerative diseases are a growing public health challenge, and amyotrophic lateral sclerosis (ALS) remains a fatal incurable disease. The advent of stem cell therapy has opened new horizons for both researchers and ALS patients, desperately looking for a treatment. ALS must be considered a systemic disease affecting many cell phenotypes besides motor neurons, even outside the central nervous system. Cell replacement therapy needs to address the specific neurobiological issues of ALS to safely and efficiently reach clinical settings. Moreover, the enormous potential of induc...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Mesenchymal stem cells in the treatment of ischemic stroke: progress and possibilities.
Authors: Doeppner TR, Hermann DM Abstract Stroke is a major cause of death and long-term disability in industrialized countries, and the only causal therapy for stroke comprises recombinant tissue plasminogen activator(rt-PA)-mediated recanalization of the occluded vessel. New experimental strategies focus on neuroregenerative approaches, among which the application of mesenchymal stem cells (MSCs) has gained increasing attention. MSCs, like other stem cells, have the capacity of unlimited self-renewal giving rise to differentiated cells from various cell lineages. Bone marrow (BM)-derived MSCs are the most frequen...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells.
In this study, we investigated the effects of serum on glia and oligodendrocyte differentiation from human mesenchymal stem cells (KP-hMSCs). We found that serum deprivation resulted in a reversible downregulation of glial- and oligodendrocyte-specific markers. Serum stimulated expression of oligodendrocyte markers, such as galactocerebroside, as well as Notch1 and JAK1 transcripts. Inhibition of Notch1 activation by the Notch inhibitor, MG132, led to enhanced expression of a serum-stimulated oligodendrocyte marker. This marker was undetectable in serum-deprived KP-hMSCs treated with MG132, suggesting that inhibition of No...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Neural stem cell sex dimorphism in aromatase (CYP19) expression: a basis for differential neural fate.
CONCLUSION: Our results confirm our previous data that the neural phenotype acquired by differentiating NSCs largely depends on cell sex, and that differential expression of aromatase in undifferentiated NSCs might contribute to this sex-based dimorphism. Although still preliminary, our discovery may have clinical application in the development of future brain repair strategies. PMID: 24198523 [PubMed] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Potential sources of stem cells as a regenerative therapy for Parkinson's disease.
Authors: El-Sadik AO Abstract Stem cells are believed to hold enormous promise as potential replacement therapy in the treatment of neurodegenerative diseases such as Parkinson's disease (PD). Stem cells were investigated to be the alternative therapeutic source capable of differentiating into dopamine (DA) neurons. Multiple important signaling factors were recorded for the induction of DA neuronal traits from mouse embryonic stem cells (ESCs) such as fibroblast growth factor 8, sonic hedgehog, and Wnt 1. Recent protocols were described for the differentiation of human ESCs into DA neurons, achieving high efficienc...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Emerging options for the management of age-related macular degeneration with stem cells.
Authors: Mooney I, Lamotte J Abstract Age-related macular degeneration (AMD) is a devastating retinal disease that occurs in later life as the retinal pigment epithelium (RPE) cells die, with subsequent photoreceptor degeneration. In the past, RPE transplant surgeries gave evidence that AMD was potentially treatable, but it involved limited amounts of ocular tissue, and the complication rate was high. Then, stem cell transplants offered an unlimited supply of retinal precursors for endogenous repair and exogenous cell replacement. Debate continues as to which type of stem cell is most appropriate for treating AMD. ...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Induced pluripotent stem cells: advances to applications.
Authors: Nelson TJ, Martinez-Fernandez A, Yamada S, Ikeda Y, Perez-Terzic C, Terzic A Abstract Induced pluripotent stem cell (iPS) technology has enriched the armamentarium of regenerative medicine by introducing autologous pluripotent progenitor pools bioengineered from ordinary somatic tissue. Through nuclear reprogramming, patient-specific iPS cells have been derived and validated. Optimizing iPS-based methodology will ensure robust applications across discovery science, offering opportunities for the development of personalized diagnostics and targeted therapeutics. Here, we highlight the process of nuclear rep...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Increased mobilization and yield of stem cells using plerixafor in combination with granulocyte-colony stimulating factor for the treatment of non-Hodgkin's lymphoma and multiple myeloma.
Authors: Pelus LM, Farag SS Abstract Multiple myeloma and non-Hodgkin's lymphoma remain the most common indications for high-dose chemotherapy and autologous peripheral blood stem cell rescue. While a CD34+ cell dose of 1 × 10(6)/kg is considered the minimum required for engraftment, higher CD34+ doses correlate with improved outcome. Numerous studies, however, support targeting a minimum CD34+ cell dose of 2.0 × 10(6)/kg, and an "optimal" dose of 4 to 6 × 10(6)/kg for a single transplant. Unfortunately, up to 40% of patients fail to mobilize an optimal CD34+ cell dose using myeloid grow...
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Erratum: Gene transfection in primary stem-like cells of giant cell tumor of bone.
Authors: Abstract [This corrects the article on p. 129 in vol. 3.]. PMID: 24198527 [PubMed - as supplied by publisher] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research

Age and sex differences in neural stem cell transplantation: a descriptive study in rats.
CONCLUSION: The outcome of the NSC transplantation was highly dependent on the sex and age of the combination donor/recipient. Data generated from our work may allow us in the future to answer the question "What NSCs and for whom?" and consequently lead to the optimization of the grafting process and improvement of the clinical prognosis. PMID: 24198528 [PubMed] (Source: Stem Cells and Cloning: Advances and Applications)
Source: Stem Cells and Cloning: Advances and Applications - December 1, 2015 Category: Stem Cells Tags: Stem Cells Cloning Source Type: research