Inter-strain variability on the cardinal parameters (pH and A < sub > w < /sub > ) of clinical and food isolates of Listeria monocytogenes using turbidimetric measurements
This study aims to gain knowledge about the inter-strain variability of twenty-six clinical and food L. monocytogenes isolates with the estimation of their cardinal parameters using turbidimetric measurements. To analyse the data and to obtain the estimated cardinal values, a common statistical procedure was set up. The estimation of cardinal parameters showed a high inter-strain variability of L. monocytogenes, and no correlation was observed between Aw min and pHmin values for the studied strains. By grouping the strains in clinical, meat and fish origin, it was observed that strains from the meat group presented the low...
Source: International Journal of Food Microbiology - December 20, 2023 Category: Food Science Authors: Salud Mar ía Serrano Heredia Yvan Le Marc Javier S ánchez Martín Ar ícia Possas Elena Carrasco Jim énez Antonio Valero D íaz Source Type: research

Characteristics of famoxadone-resistant mutants of Phytophthora litchii and their effect on lychee fruit quality
In this study, we determined the sensitivity of 133 P. litchii isolates to famoxadone, yielding a mean EC50 value of 0.46 ± 0.21 μg/mL. Through fungicide adaption, we derived resistant mutants with M124I and Y131C substitutions in PlCyt b (Cytochrome b in P. litchii) from wild-type isolates. In vitro assessments revealed that the fitness of the resistant mutants was significantly lower compared to the parental isolates. These laboratory findings demonstrate a moderate resistance risk of P. litchii to famoxadone. Molecular docking analyses indicated that the M124I and Y131C alterations disrupted hydrogen bonds and weakene...
Source: International Journal of Food Microbiology - December 20, 2023 Category: Food Science Authors: Jianqiang Miao Xuheng Gao Yidong Tang Tan Dai Xili Liu Source Type: research

Evaluation of universal preenrichment broth and comparison of rapid molecular methods for the detection of Salmonella from spent sprout irrigation water (SSIW)
This study aimed to compare universal preenrichment broth (UPB) and lactose broth (LB) as preenrichment media for cultural and rapid screening methods and to compare their abilities to recover Salmonella in SSIW samples from different sprout varieties (i.e., alfalfa, broccoli, and mung bean sprouts). The associated co-enriched microbiota with different sprout varieties using different preenrichment media were also examined using a quasi-metagenomic approach. The performance of media and detection methods was compared using the relative level of detection (RLOD) value, as recommended by ISO 16140-2:2016. The level of detect...
Source: International Journal of Food Microbiology - December 20, 2023 Category: Food Science Authors: Jie Zheng Elizabeth Reed Anna Maounounen-Laasri Xiaohong Deng Shizhen S Wang Padmini Ramachandran Christina Ferreira Rebecca Bell Eric W Brown Thomas S Hammack Hua Wang Source Type: research

Effect of chlorogenic acid grafted chitosan on microbiological compositions of sea bass (Lateolabrax japonicus) fillets: Dominant spoilage bacteria, inhibition activity and membrane damage mechanisms
This study investigated the effect of chlorogenic acid grafted chitosan (CS-g-CA) on the microbiota composition of sea bass (Lateolabrax japonicus), isolated and identified the specific spoilage organisms (SSOs) in the late stage of refrigerated fillets and evaluation of their spoilage potential. Moreover, antibacterial activity and membrane damage mechanism of CS-g-CA against spoilage bacteria was also investigated. Illumina-MiSeq high throughput sequencing results showed that CS-g-CA retarded the growth of Pseudomonas spp., which largely contributed to delaying the quality degradation of sea bass during storage. Then nin...
Source: International Journal of Food Microbiology - December 20, 2023 Category: Food Science Authors: Xin Yang Weiqing Lan Xiaohong Sun Source Type: research

Campylobacter species and genotype distribution in Finnish beef liver - Retail liver juice ideal for isolation and quantification
Int J Food Microbiol. 2023 Dec 14;411:110524. doi: 10.1016/j.ijfoodmicro.2023.110524. Online ahead of print.ABSTRACTCampylobacteriosis, primarily caused by Campylobacter jejuni and C. coli, is the main bacterial zoonosis worldwide. While poultry is recognized as the main reservoir, bovines are considered another important reservoir for Campylobacter spp. found in human infections. In contrast to chicken, retail beef is seldom contaminated by Campylobacter species. However, beef liver is recognized to be frequently contaminated and has been linked to human infections via epidemiological investigations. Our aims were to eval...
Source: International Journal of Food Microbiology - December 20, 2023 Category: Food Science Authors: Jenna Luoto Riikka Keto-Timonen Rauni Kivist ö Source Type: research

Inter-strain variability on the cardinal parameters (pH and A < sub > w < /sub > ) of clinical and food isolates of Listeria monocytogenes using turbidimetric measurements
This study aims to gain knowledge about the inter-strain variability of twenty-six clinical and food L. monocytogenes isolates with the estimation of their cardinal parameters using turbidimetric measurements. To analyse the data and to obtain the estimated cardinal values, a common statistical procedure was set up. The estimation of cardinal parameters showed a high inter-strain variability of L. monocytogenes, and no correlation was observed between Aw min and pHmin values for the studied strains. By grouping the strains in clinical, meat and fish origin, it was observed that strains from the meat group presented the low...
Source: International Journal of Food Microbiology - December 20, 2023 Category: Food Science Authors: Salud Mar ía Serrano Heredia Yvan Le Marc Javier S ánchez Martín Ar ícia Possas Elena Carrasco Jim énez Antonio Valero D íaz Source Type: research

Characteristics of famoxadone-resistant mutants of Phytophthora litchii and their effect on lychee fruit quality
In this study, we determined the sensitivity of 133 P. litchii isolates to famoxadone, yielding a mean EC50 value of 0.46 ± 0.21 μg/mL. Through fungicide adaption, we derived resistant mutants with M124I and Y131C substitutions in PlCyt b (Cytochrome b in P. litchii) from wild-type isolates. In vitro assessments revealed that the fitness of the resistant mutants was significantly lower compared to the parental isolates. These laboratory findings demonstrate a moderate resistance risk of P. litchii to famoxadone. Molecular docking analyses indicated that the M124I and Y131C alterations disrupted hydrogen bonds and weakene...
Source: International Journal of Food Microbiology - December 20, 2023 Category: Food Science Authors: Jianqiang Miao Xuheng Gao Yidong Tang Tan Dai Xili Liu Source Type: research

Evaluation of universal preenrichment broth and comparison of rapid molecular methods for the detection of Salmonella from spent sprout irrigation water (SSIW)
This study aimed to compare universal preenrichment broth (UPB) and lactose broth (LB) as preenrichment media for cultural and rapid screening methods and to compare their abilities to recover Salmonella in SSIW samples from different sprout varieties (i.e., alfalfa, broccoli, and mung bean sprouts). The associated co-enriched microbiota with different sprout varieties using different preenrichment media were also examined using a quasi-metagenomic approach. The performance of media and detection methods was compared using the relative level of detection (RLOD) value, as recommended by ISO 16140-2:2016. The level of detect...
Source: International Journal of Food Microbiology - December 20, 2023 Category: Food Science Authors: Jie Zheng Elizabeth Reed Anna Maounounen-Laasri Xiaohong Deng Shizhen S Wang Padmini Ramachandran Christina Ferreira Rebecca Bell Eric W Brown Thomas S Hammack Hua Wang Source Type: research

Effect of chlorogenic acid grafted chitosan on microbiological compositions of sea bass (Lateolabrax japonicus) fillets: Dominant spoilage bacteria, inhibition activity and membrane damage mechanisms
This study investigated the effect of chlorogenic acid grafted chitosan (CS-g-CA) on the microbiota composition of sea bass (Lateolabrax japonicus), isolated and identified the specific spoilage organisms (SSOs) in the late stage of refrigerated fillets and evaluation of their spoilage potential. Moreover, antibacterial activity and membrane damage mechanism of CS-g-CA against spoilage bacteria was also investigated. Illumina-MiSeq high throughput sequencing results showed that CS-g-CA retarded the growth of Pseudomonas spp., which largely contributed to delaying the quality degradation of sea bass during storage. Then nin...
Source: International Journal of Food Microbiology - December 20, 2023 Category: Food Science Authors: Xin Yang Weiqing Lan Xiaohong Sun Source Type: research

Campylobacter species and genotype distribution in Finnish beef liver - Retail liver juice ideal for isolation and quantification
Int J Food Microbiol. 2023 Dec 14;411:110524. doi: 10.1016/j.ijfoodmicro.2023.110524. Online ahead of print.ABSTRACTCampylobacteriosis, primarily caused by Campylobacter jejuni and C. coli, is the main bacterial zoonosis worldwide. While poultry is recognized as the main reservoir, bovines are considered another important reservoir for Campylobacter spp. found in human infections. In contrast to chicken, retail beef is seldom contaminated by Campylobacter species. However, beef liver is recognized to be frequently contaminated and has been linked to human infections via epidemiological investigations. Our aims were to eval...
Source: International Journal of Food Microbiology - December 20, 2023 Category: Food Science Authors: Jenna Luoto Riikka Keto-Timonen Rauni Kivist ö Source Type: research

Biofilm formation and desiccation survival of Listeria monocytogenes with microbiota on mushroom processing surfaces and the effect of cleaning and disinfection
This study shows that L. monocytogenes is able to form single and multispecies biofilms on PVC with high strain diversity following C&D treatments. This highlights the need to apply more stringent C&D regime treatments for especially PVC and similar surfaces to efficiently remove biofilm cells from food processing surfaces.PMID:38101188 | DOI:10.1016/j.ijfoodmicro.2023.110509 (Source: International Journal of Food Microbiology)
Source: International Journal of Food Microbiology - December 15, 2023 Category: Food Science Authors: Frank B Lake Jingjie Chen Leo S van Overbeek Johan J P Baars Tjakko Abee Heidy M W den Besten Source Type: research

BolA promotes the generation of multicellular behavior in S. Typhimurium by regulating the c-di-GMP pathway genes yeaJ and yhjH
In conclusion, BolA mainly improves the content of extracellular polysaccharide by promoting the expression of yeaJ, thus enhancing the formation of biofilms. BolA also restricts flagellar synthesis by inhibiting yhjH expression, therefore reducing motility, ultimately promoting multicellular behavior arises. These findings lay a theoretical foundation for the prevention and control of S. Typhimurium.PMID:38101189 | DOI:10.1016/j.ijfoodmicro.2023.110518 (Source: International Journal of Food Microbiology)
Source: International Journal of Food Microbiology - December 15, 2023 Category: Food Science Authors: Kaifeng Chen Lili Li Zhouping Zhou Nanwei Wang Changzhi Dai Dage Sun Jiayi Li Chenggang Xu Ming Liao Jianmin Zhang Source Type: research

Application of combined treatment of peracetic acid and ultraviolet-C for inactivating pathogens in water and on surface of apples
In this study, a combined treatment of peracetic acid (PAA) and 280 nm Ultraviolet-C (UVC) - Light emitting diode (LED) was applied for inactivating foodborne pathogens in water and apples. The combined treatment of PAA (50 ppm) and UVC-LED showed synergistic inactivation effects against Escherichia coli O157:H7 and Listeria monocytogenes in water. In mechanism analysis, PAA/UVC-LED treatment induced more lipid peroxidation, intracellular ROS, membrane, and DNA damage than a single treatment. Among them, membrane damage was the main synergistic inactivation mechanism of combination treatment. Cell rupture and shrink of bot...
Source: International Journal of Food Microbiology - December 15, 2023 Category: Food Science Authors: Minjung Shin Gyumi Na Jun-Won Kang Dong-Hyun Kang Source Type: research

Biofilm formation and desiccation survival of Listeria monocytogenes with microbiota on mushroom processing surfaces and the effect of cleaning and disinfection
This study shows that L. monocytogenes is able to form single and multispecies biofilms on PVC with high strain diversity following C&D treatments. This highlights the need to apply more stringent C&D regime treatments for especially PVC and similar surfaces to efficiently remove biofilm cells from food processing surfaces.PMID:38101188 | DOI:10.1016/j.ijfoodmicro.2023.110509 (Source: International Journal of Food Microbiology)
Source: International Journal of Food Microbiology - December 15, 2023 Category: Food Science Authors: Frank B Lake Jingjie Chen Leo S van Overbeek Johan J P Baars Tjakko Abee Heidy M W den Besten Source Type: research

BolA promotes the generation of multicellular behavior in S. Typhimurium by regulating the c-di-GMP pathway genes yeaJ and yhjH
In conclusion, BolA mainly improves the content of extracellular polysaccharide by promoting the expression of yeaJ, thus enhancing the formation of biofilms. BolA also restricts flagellar synthesis by inhibiting yhjH expression, therefore reducing motility, ultimately promoting multicellular behavior arises. These findings lay a theoretical foundation for the prevention and control of S. Typhimurium.PMID:38101189 | DOI:10.1016/j.ijfoodmicro.2023.110518 (Source: International Journal of Food Microbiology)
Source: International Journal of Food Microbiology - December 15, 2023 Category: Food Science Authors: Kaifeng Chen Lili Li Zhouping Zhou Nanwei Wang Changzhi Dai Dage Sun Jiayi Li Chenggang Xu Ming Liao Jianmin Zhang Source Type: research