A user's guide to the bioinformatic analysis of shotgun metagenomic sequence data for bacterial pathogen detection
Int J Food Microbiol. 2023 Nov 17;410:110488. doi: 10.1016/j.ijfoodmicro.2023.110488. Online ahead of print.ABSTRACTMetagenomics, i.e., shotgun sequencing of the total microbial community DNA from a sample, has become a mature technique but its application to pathogen detection in clinical, environmental, and food samples is far from common or standardized. In this review, we summarize ongoing developments in metagenomic sequence analysis that facilitate its wider application to pathogen detection. We examine theoretical frameworks for estimating the limit of detection for a particular level of sequencing effort, current a...
Source: International Journal of Food Microbiology - November 30, 2023 Category: Food Science Authors: Blake G Lindner Kenji Gerhardt Dorian J Feistel Luis M Rodriguez-R Janet K Hatt Konstantinos T Konstantinidis Source Type: research

Corrigendum to "From farm to fork: Spread of a multidrug resistant Salmonella Infantis clone encoding blaCTX-M-1 on pESI-like plasmids in Central Italy" [Int. J. Food Microbiol. volume 410 (2024) 1-11/110490]
Int J Food Microbiol. 2023 Nov 29:110510. doi: 10.1016/j.ijfoodmicro.2023.110510. Online ahead of print.NO ABSTRACTPMID:38036318 | DOI:10.1016/j.ijfoodmicro.2023.110510 (Source: International Journal of Food Microbiology)
Source: International Journal of Food Microbiology - November 30, 2023 Category: Food Science Authors: Ilaria Russo Jennie Fischer Laura Uelze Maira Napoleoni Giuditta Fiorella Schiavano Francesca Andreoni Giorgio Brandi Giulia Amagliani Source Type: research

Spherical Fe < sub > 2 < /sub > O < sub > 3 < /sub > nanoparticles inhibit the production of aflatoxins (B < sub > 1 < /sub > and B < sub > 2 < /sub > ) and regulate total soluble solids and titratable acidity of peach fruit
This study was carried out to assess the levels of aflatoxin contamination in diseased peach (Prunus persica L.) fruit and their control using myco-synthesized iron oxide nanoparticles (Fe2O3 NPs). Diseased peach fruit were diagnosed to be infected with Aspergillus flavus. The isolated pathogen was cultured under UV light (365 nm) and exposed to ammonium hydroxide (31 %) vapors, which confirmed its ability to produce aflatoxin. For the control of this disease, Fe2O3 NPs were synthesized in the filtrate of a biocontrol fungus (Trichoderma harzianum) and characterized before analyzing their potential in disease control. FTIR...
Source: International Journal of Food Microbiology - November 29, 2023 Category: Food Science Authors: Mahnoor Akbar Naeem Ali Muhammad Imran Arshad Hussain Syed Waqas Hassan Urooj Haroon Asif Kamal None Farhana Hassan Javed Chaudhary Muhammad Farooq Hussain Munis Source Type: research

Genome-wide transcriptome analysis of toxigenic Fusarium verticillioides in response to variation of temperature and water activity on maize kernels
In this study, DEGs of F. verticillioides and their related regulatory pathways were analyzed in response to the stress of temperature and aw combinations using RNA-Seq. The results showed that the optimal growth conditions for F. verticillioides were 0.98 aw and 25 °C, whereas the highest per-unit yield of the fumonisin B1 (FB1) was observed at 0.98 aw and 15 °C. The RNA-seq analysis showed that 9648 DEGs were affected by temperature regardless of aw levels, whereas only 218 DEGs were affected by aw regardless of temperature variations. Gene Ontology (GO) analysis revealed that a decrease in temperature at both aw level...
Source: International Journal of Food Microbiology - November 25, 2023 Category: Food Science Authors: Yi Ding Nini Ma Hafiz Abdul Haseeb Zhaoji Dai Jun Zhang Wei Guo Source Type: research

Genome-wide transcriptome analysis of toxigenic Fusarium verticillioides in response to variation of temperature and water activity on maize kernels
In this study, DEGs of F. verticillioides and their related regulatory pathways were analyzed in response to the stress of temperature and aw combinations using RNA-Seq. The results showed that the optimal growth conditions for F. verticillioides were 0.98 aw and 25 °C, whereas the highest per-unit yield of the fumonisin B1 (FB1) was observed at 0.98 aw and 15 °C. The RNA-seq analysis showed that 9648 DEGs were affected by temperature regardless of aw levels, whereas only 218 DEGs were affected by aw regardless of temperature variations. Gene Ontology (GO) analysis revealed that a decrease in temperature at both aw level...
Source: International Journal of Food Microbiology - November 25, 2023 Category: Food Science Authors: Yi Ding Nini Ma Hafiz Abdul Haseeb Zhaoji Dai Jun Zhang Wei Guo Source Type: research

Genome-wide transcriptome analysis of toxigenic Fusarium verticillioides in response to variation of temperature and water activity on maize kernels
In this study, DEGs of F. verticillioides and their related regulatory pathways were analyzed in response to the stress of temperature and aw combinations using RNA-Seq. The results showed that the optimal growth conditions for F. verticillioides were 0.98 aw and 25 °C, whereas the highest per-unit yield of the fumonisin B1 (FB1) was observed at 0.98 aw and 15 °C. The RNA-seq analysis showed that 9648 DEGs were affected by temperature regardless of aw levels, whereas only 218 DEGs were affected by aw regardless of temperature variations. Gene Ontology (GO) analysis revealed that a decrease in temperature at both aw level...
Source: International Journal of Food Microbiology - November 25, 2023 Category: Food Science Authors: Yi Ding Nini Ma Hafiz Abdul Haseeb Zhaoji Dai Jun Zhang Wei Guo Source Type: research

Genome-wide transcriptome analysis of toxigenic Fusarium verticillioides in response to variation of temperature and water activity on maize kernels
In this study, DEGs of F. verticillioides and their related regulatory pathways were analyzed in response to the stress of temperature and aw combinations using RNA-Seq. The results showed that the optimal growth conditions for F. verticillioides were 0.98 aw and 25 °C, whereas the highest per-unit yield of the fumonisin B1 (FB1) was observed at 0.98 aw and 15 °C. The RNA-seq analysis showed that 9648 DEGs were affected by temperature regardless of aw levels, whereas only 218 DEGs were affected by aw regardless of temperature variations. Gene Ontology (GO) analysis revealed that a decrease in temperature at both aw level...
Source: International Journal of Food Microbiology - November 25, 2023 Category: Food Science Authors: Yi Ding Nini Ma Hafiz Abdul Haseeb Zhaoji Dai Jun Zhang Wei Guo Source Type: research

Predicting Listeria monocytogenes virulence potential using whole genome sequencing and machine learning
Int J Food Microbiol. 2023 Nov 17;410:110491. doi: 10.1016/j.ijfoodmicro.2023.110491. Online ahead of print.ABSTRACTContamination with food-borne pathogens, such as Listeria monocytogenes, remains a big concern for food safety. Hence, rigorous and continuous microbial surveillance is a standard procedure. At this point, however, the food industry and authorities only focus on detection of Listeria monocytogenes without characterization of individual strains into groups of more or less concern. As whole genome sequencing (WGS) gains increasing interest in the industry, this methodology presents an opportunity to obtain fine...
Source: International Journal of Food Microbiology - November 24, 2023 Category: Food Science Authors: Alexander Gmeiner Patrick Murigu Kamau Njage Lisbeth Truelstrup Hansen Frank M Aarestrup Pimlapas Leekitcharoenphon Source Type: research

Predicting Listeria monocytogenes virulence potential using whole genome sequencing and machine learning
Int J Food Microbiol. 2023 Nov 17;410:110491. doi: 10.1016/j.ijfoodmicro.2023.110491. Online ahead of print.ABSTRACTContamination with food-borne pathogens, such as Listeria monocytogenes, remains a big concern for food safety. Hence, rigorous and continuous microbial surveillance is a standard procedure. At this point, however, the food industry and authorities only focus on detection of Listeria monocytogenes without characterization of individual strains into groups of more or less concern. As whole genome sequencing (WGS) gains increasing interest in the industry, this methodology presents an opportunity to obtain fine...
Source: International Journal of Food Microbiology - November 24, 2023 Category: Food Science Authors: Alexander Gmeiner Patrick Murigu Kamau Njage Lisbeth Truelstrup Hansen Frank M Aarestrup Pimlapas Leekitcharoenphon Source Type: research

Effects of ohmic heating with different voltages on the quality and microbial diversity of cow milk during thermal treatment and subsequent cold storage
Int J Food Microbiol. 2023 Nov 10;410:110483. doi: 10.1016/j.ijfoodmicro.2023.110483. Online ahead of print.ABSTRACTOhmic heating (OH), an innovative heating technology, presents potential applications in the pasteurization of liquid foods. Therefore, the study was conducted to evaluate the effect of OH at various voltage gradients (10 V/cm, 12.5 V/cm, and 15 V/cm) and water bath (WB) on microbial inactivation, physicochemical and sensory properties and microbial flora of pasteurized milk. Results indicated that OH with higher voltage could effectively inactivate microorganisms in milk, requiring less heating time and ener...
Source: International Journal of Food Microbiology - November 23, 2023 Category: Food Science Authors: Yingying Sun Yana Liu Weiwei Zhou Lele Shao Han Wang Yijie Zhao Bo Zou Xingmin Li Ruitong Dai Source Type: research

Effects of ohmic heating with different voltages on the quality and microbial diversity of cow milk during thermal treatment and subsequent cold storage
Int J Food Microbiol. 2023 Nov 10;410:110483. doi: 10.1016/j.ijfoodmicro.2023.110483. Online ahead of print.ABSTRACTOhmic heating (OH), an innovative heating technology, presents potential applications in the pasteurization of liquid foods. Therefore, the study was conducted to evaluate the effect of OH at various voltage gradients (10 V/cm, 12.5 V/cm, and 15 V/cm) and water bath (WB) on microbial inactivation, physicochemical and sensory properties and microbial flora of pasteurized milk. Results indicated that OH with higher voltage could effectively inactivate microorganisms in milk, requiring less heating time and ener...
Source: International Journal of Food Microbiology - November 23, 2023 Category: Food Science Authors: Yingying Sun Yana Liu Weiwei Zhou Lele Shao Han Wang Yijie Zhao Bo Zou Xingmin Li Ruitong Dai Source Type: research

Impact of vitamin B < sub > 12 < /sub > on rhamnose metabolism, stress defense and in-vitro virulence of Listeria monocytogenes
In this study we investigated the impact of B12 and BMC activation on i) aerobic and anerobic growth of L. monocytogenes on rhamnose and ii) the level of virulence. We observed B12-induced pdu BMC activation and growth stimulation only in anaerobically grown cells. Comparative Caco-2 virulence assays showed that these pdu BMC-induced cells have significantly higher translocation efficiency compared to non-induced cells (anaerobic growth without B12; aerobic growth with or without B12), while adhesion and invasion capacity is similar for all cells. Comparative proteome analysis showed specific and overlapping responses link...
Source: International Journal of Food Microbiology - November 22, 2023 Category: Food Science Authors: Zhe Zeng Lucas M Wijnands Sjef Boeren Eddy J Smid Richard A Notebaart Tjakko Abee Source Type: research

From farm to fork: Spread of a multidrug resistant Salmonella Infantis clone encoding bla < sub > CTX-M-1 < /sub > on pESI-like plasmids in Central Italy
Int J Food Microbiol. 2023 Nov 17;410:110490. doi: 10.1016/j.ijfoodmicro.2023.110490. Online ahead of print.ABSTRACTSalmonella enterica subsp. enterica serovar Infantis (S. Infantis) is one of the "top five Salmonella serovars" of clinical significance in the European Union (EU). Antimicrobial resistant and extended spectrum β-lactamase (ESBL) AmpC-producing S. Infantis have been described in food production systems and human clinical samples in Italy. Recently, an increase of MDR S. Infantis carrying blaCTX-M genes involved in 3rd generation cephalosporin resistance was noticed in the EU, including Italy, mainly due to t...
Source: International Journal of Food Microbiology - November 22, 2023 Category: Food Science Authors: Ilaria Russo Jennie Fischer Laura Uelze Maira Napoleoni Giuditta Fiorella Schiavano Francesca Andreoni Giorgio Brandi Giulia Amagliani Source Type: research

Contamination of Fusarium spp. and mycotoxins at different ear physiological stages of maize in Argentina
Int J Food Microbiol. 2023 Nov 14;410:110493. doi: 10.1016/j.ijfoodmicro.2023.110493. Online ahead of print.ABSTRACTMaize is one of the most important crops worldwide, being affected by several fungal species under field conditions. The study of plant-pathogen interaction plays a key role because fungal diseases are responsible for reducing grain yield and quality by increasing mycotoxin production. Thus, the present work aimed to evaluate the interaction of F. graminearum and F. verticillioides and mycotoxin production under field conditions along different physiological stages. During the 2019/2020 and 2020/2021 growing ...
Source: International Journal of Food Microbiology - November 21, 2023 Category: Food Science Authors: Agust ín F Arata Mauro Mart ínez Natalia V Pesquero Diego Cristos Mar ía I Dinolfo Source Type: research

Detection of hepatitis E RNA in pork products at point of retail in Ireland - Are consumers at risk?
Int J Food Microbiol. 2023 Nov 17;410:110492. doi: 10.1016/j.ijfoodmicro.2023.110492. Online ahead of print.ABSTRACTHepatitis E (HEV), a zoonotic virus, is the leading cause of acute viral hepatitis in Europe. The presence of HEV in domestic pigs can result in infections in humans through consumption of pork products which are undercooked or where processing methods are insufficient to inactivate the virus. In Ireland, pork accounts for 34 % of all meat consumption (CSO, 2022) and the prevalence of HEV in products at point of retail has not previously been characterised. A sampling strategy was designed in which high pork ...
Source: International Journal of Food Microbiology - November 21, 2023 Category: Food Science Authors: Charlene Bennett Suzie Coughlan Kevin Hunt Francis Butler S éamus Fanning Eoin Ryan Cillian De Gascun Joanne O'Gorman Source Type: research